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Environmental Health Hazard of Arsenic on Epididymis and Ductus Deferens in Male Black Bengal Goats

Ms.Morsheda Nasrin
Associate Professor
Department of Anatomy and Histology, Faculty of Veterinary Science,Bangladesh Agricultural University, Mymensingh-2202,Bangladesh

MA Wares
Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202 Bangladesh

MA Awal
Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202 Bangladesh

MNH Siddiqi
Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202 Bangladesh

Abstract/ Executive Summary:

Histomorphological changes of epididymis and ductus deferens in male Black Bengal goat due to arsenic were studied. A total of 12 male Black Bengal goats, in which 6 were collected from arsenic affected areas of Mymensingh district and another 6 were collected from hill tracts of Chittagong which were arsenic free.The goats were sacrificed by piercing carotid artery and the samples (Epididymis and Ductus deferens) were collected immediately. Samples were prepared and stained with haematoxylin and eosin stain technique to study the histology under light microscope. In the morphological study, measurement of length, width, breadth and weight of epididymis and ductus deferens were observed. The gross study revealed that there were slight variations in the gross morphology of epididymis and ductus deferens of arsenic affected Black Bengal goat, but this variation was statistically insignificant. In the histological study, arsenic affected goat showing increased thickness of epididymal covering (P<0.05) and trabeculae compared to control group of epididymis. The diameter of ductule efferentes of arsenic affected goat was narrower (P<0.01), width between intertubular space of ductuli efferentes was wider (P<0.01), lumen of ductules contain smaller in amount of spermatozoa compared to control group. The wall of the ductus deferens was thicker in arsenic affected goats than the control group (P<0.01). It may be concluded that environmental health hazard of arsenic might have adverse effects on the male reproductive organs.

Keywords: Arsenic, Morphology, Histology, Epididymis, Ductus deferens, Black Bengal goat

Location: Mymensingh

Start Date: 00-00-2012

End Date:

Program Area: Animal Health and Management

Commodity: Goat

Objectives:

To observe the environmental health hazard of arsenic on epididymis and ductus deferens in Black Bengal goats.

Methodology:

A total of 12 mature male Black Bengal goats were used in this study. Six goats were collected from arsenic affected areas of Mymensingh district in the year 2012 and another six from arsenic free areas of hill tracts of Chittagong. The goats were sacrificed by piercing carotid artery and the samples (Epididymis and Ductus deferens) were collected immediately. Soon after collection of samples, gross parameters (Colour, length, width, breadth and weight) of epididymis and ductus deferens were observed and recorded. For histological studies, the samples were cut into small pieces and then fixed in the “Bouins fluid” (Gridley, 1960) for 18-24 hours and were dehydrated in the series of ascending grade of alcohol followed by clearing in three changes in xylene, and the tissues then infiltrated with different grades of melted paraffin in the oven (M. T. Islam et al., 2011). The tissues were then embedded in paraffin and finally the sections were cut at 6 μm thickness using sliding microtome (MIC 509, Euromex, Japan). After cutting, the sections were floated on luke-warm water in a floatation bath at 37⁰C for stretching and then the sections were mounted on clean slides using an adhesive (Egg albumins) and dried on a slide warmer. The sections were stained using Mayer’s Hematoxylin and Eosin (H & E) stain. The stuctures were examined by using light microscope under low 10x, 20x and high 100x magnification. Measurements of structures were taken by the calibrated stage micrometer and photograph was taken for better illustration. The recorded data were compiled and tabulated for statistical analysis by the help of Computer package SPSS 11. The mean was compared in one-way ANOVA Method.

Source of Information: J. Bangladesh Agril. Univ. 11(1): 103–110, 2013

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

It is suggested that environmental health hazard of arsenic adversely affects the male reproductive organs (epididymis and ductus deferens) of Black Bengal goats, causing infertility.

Knowledge Management: Journal