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Effects of Probiotics and Enzymes on Growth Performance and Haematobiochemical Parameters in Broilers

Mr. Md. S. Rahman
MS Fellow
Department of Physiology, Bangladesh Agricultural University, Mymensingh-2202Bangladesh

Abstract/ Executive Summary:

An study was conducted on "Cobb-500" broiler chicks to evaluate the effects of probiotics and enzymes supplementation on growth performance, haematological and biochemical parameters. A total of 20, fourteen days old broiler chicks were divided randomly into four groups using five broiler chicks in each group. Group A was designated as control group was given only normal broiler ration and rest of the groups were fed probiotics and enzymes, [Group B (probiotic Microguard @ 1 gm/L), Group C (enzymes preparation Acmezyme* 1 gm/L), Group D (Microguard* g 1 gm/L and Acmezyme @ 1 gm/L)] with drinking water respectively from 1^Stto 21^st day of study. It was observed that probiotics and enzymes supplementation enhanced the body growth rate. At the final day of experiment the body weight was significantly increased (P<0.01) in the treated groups in comparison with that of control group. Total Erythrocyte Count (TEC), Hemoglobin (Hb) concentration, Packed Cell Volume (PCV) were significantly increased (p<0.01) in treated groups than the control group. Erythrocyte Sedimentation Rate (ESR) and Serum alanine aminotransaminase (ALT) levels were decreased significantly (p<0.01) than the control group. The serum Serum aspertate aminotransaminase (AST) level was decreased significantly (p<0.01) in all treated groups except group D than the control group. It is suggests that the poultry farming may be benefited using probiotics and enzymes.

Keywords: Broiler, Growth, Hematological parameters, Biochemical

Location: Mymensingh

Start Date: 09-04-2012

End Date: 06-05-2012

Program Area: Animal Health and Management

Commodity: Chicken

Objectives:

i) To evaluate the effects of probiotics and enzymes supplementation on growth performance, haematological and biochemical parameter

ii) To study the effects of probiotics and enzymes supplementation on growth performance in broiler chicks

iii) To study the effect of probiotics and enzymes supplementation on haematological parameters.

Methodology:

The study was conducted in Hamidur Poultry Farm, Mahigani, Rangpur during the period from 9th April to 6th May in 2012.Experimental birds: Day old chicks (strain Cobb-500) marketed by Nourish Poultry and Hachery Ltd., Bangladesh, were purchased from local market for this experiment. The chickens were allowed to take rest for 6 days for the adaptation. The chicken was supplied with normal diet and water. Experimental diets: The commercial broiler ration, commercial enzymes (Achmezyme®) and commercial probiotics (Microgulard®) were purchase from the local market. Group–A : fed with commercial broiler ration and fresh drinking water, Group–B : commercial probiotics (Microguard®) @ 1gm/L drinking water plus commercial broiler ration and fresh drinking water for 21 days, Croup–C : commercial enzymes (Achmezyme®) @1gm/L drinking water plus commercial broiler ration and fresh drinking water for 21 days and Group–D : commercial probiotics (Microguard) @ 1gm/L and enzymes (Achmezyme) @1gm/L drinking water plus commercial broiler ration and fresh drinking water for 21 days. Biosecurity and sanitation Proper hygienic and sanitation programs were followed. To prevent the outbreak of disease strict biosecurity was maintained. The following measures were taken to maintain the biosecurity:• Visitors were not allowed to enter in the house. • All equipments in the experimental house were kept clean. • Dead birds were removed promptly. Blood collection For studying haematological and biochemical parameters blood was collected at the end of study. A number of sterile test tubes containing anticoagulant (4% trisodium citrate solution) at a ratio of 1:10 were taken. Sequential killing was done and blood was collected from each group through slaughtering. The haematological studies were performed within two hours after collection. For biochemical studies blood 2- 3 ml was collected in the sterile glass test tube. The blood containing tubes were placed in slanting position at room temperature for 6 hours. The tubes were then incubated overnight in the refrigerator (4OC). The serum samples were separated and centrifuged to get rid of unwanted blood cells where necessary. Serum samples were stored at -20OC for biochemical enzyme analysis. Analyses were done in a quickest possible time. Statistical analysis The recorded data were analyzed statistically between control and treated groups of broiler chicken by the student’s test (‘t’ test).

Source of Information: J. Bangladesh Agril. Univ. 11(1): 111–118, 2013, ISSN 1810-3030

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The experiment found that Erythrocyte Sedimentation Rate (ESR) and Serum alanine aminotransaminase (ALT) levels were decreased significantly (p<0.01) than the control group. The serum Serum aspertate aminotransaminase (AST) level was decreased significantly (p<0.01) in all treated groups except group D than the control group. It is suggests that the poultry farming may be benefited using probiotics and enzymes.

Knowledge Management: Journal