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Residual Effect of Two Insecticides and Neem Oil Against Epilachna Beetle, Epilachna Vigintioctopunctata (fab.) on Bitter Gourd

Ms. Mukta Mala
M S Fellow
Department of Entomology, Patuakhali University of Science and Technology,

Abstract/ Executive Summary:

Experiments were conducted in the Laboratory and Entomology Field Laboratory to determine the residual effect of two insecticides (viz. Siperin 10EC, Malathion 57EC) and a botanical (Neem oil) against Epilachna beetle, Epilachna vigintioctopunctata (Fab.) during the period from February to May 2009. To evaluate the residual effect of one synthetic and one organophosphors pesticides and one botanical pesticide on the mortality of Epilachna beetle, different concentrations of the insecticides (1.0, 2.0 and 3.0% of Neem oil, 1.0, 1.2 and 1.5% of Malathion and 0.5, 1.0 and 1.5% of Siperin.) was used. The mortality of the beetles was recorded at 4, 5, 6 and 7 days after treatments. After 7 days the highest mortality was found on first instar larvae (70.0 in laboratory condition and 65.0 in field condition) treated by Siperin 10EC (1.5% conc.) and the lowest mortality was found on adults Epilachna beetle (17.5 in laboratory condition and 15.0 in field condition) treated by Neem oil (1.0% conc.) The results indicated that Siperin 10EC has longer residual effect than Malathion 57EC and Neem oil. The effectivity ranked: Siperin > Malathion > Neem oil.

Keywords: Residual effect, Epilachna vigintioctopunctata, Siperin, Malathion, Neem oil

Location: Mymensingh

Start Date: 00-02-2009

End Date: 00-05-2009

Program Area: Pest Management

Commodity: Insecticide

Objectives:

To investigate the residual effect of Neem oil, Malathion and Siperin for controlling epilachna beetle under laboratory and field condition.

Methodology:

The experiment was carried out in the laboratory of the Department of Entomology and Entomology Field Laboratory, Bangladesh Agricultural University (BAU), Mymensingh, during February to May, 2009. In order to meet the supply of the test insect, a stock culture of large number of grubs and adult beetles of Epilachna vigintioctopunctata was maintained in the laboratory and in the field on bitter gourd plant. Neem oil, Malathion57EC and Siperin10EC were collected from Department of Entomology, Bangladesh Agricultural University, Mymensingh. From these insecticides different concentrations were prepared with water. The mortality of E. vigintioctopunctata was evaluated by exposing first, second, third instars larvae and adult separately in Petri dish containing bitter gourd leaves treated with 1.0, 2.0 and 3.0% of Neem oil, 1.0, 1.20 and 1.5% of Malathion and 0.5, 1.0 and 1.5% of Siperin. Ten larvae and adult of E. vigintioctopunctata were supplied in each petri dish for each concentration. In control treatment, leaves were treated with distilled water only. The leaf petiole was covered with water soaked cotton pad to prevent water loss. And it was also performed in the field and in case of control plot there was no any insecticide application performed. Residual toxicity effect was evaluated by observing mortality of insects released in the treated plants and petri dishes at 4 days after treatment (DAT). Data were recorded at 4, 5, 6 and 7 days after treatment (DAT). The experiment was repeated for 3 times. The experiments laid out in a Randomized Complete Block Design (RCBD). The significance of difference between pair of means was performed by least significance difference (LSD) test taking the probability level 1 % as the maximum unit of significance. Dose response relationships were determined using Duncan's New Multiple Range Test.

Source of Information: J. Bangladesh Agril. Univ. 10(1): 21–25, 2012, ISSN 1810-3030

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The present finding indicates that all the three insecticide are effective against larval instar and adult epilachna beetle. These three insecticides caused different residual effect after 4, 5, 6 and 7 days. The rank order were Siperin > Malathion > Neem oil. It showed that Neem oil has less residual toxicity than other two insecticides. Though Neem oil had low mortality rate, its use could be considered safe for man, animals, fish and non target fauna

Knowledge Management: Journal