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Micropropagation of Year-round Jack Fruit (artocarpus Heterophyllus Lam.) Through in Vitro Culture

K. A. Ara
Horticulture Research Centre, BARI, Gazipur

S. M. Sharifuzzaman
Horticulture Research Centre, BARI, Gazipur

M.M. Hossain
Dept. of Horticulture, Bangabandhu Sheikh Mujibur Rahman Agncultural University, Gazipur

A. S. M. H. M. Talukder
Horticulture Research Centre, BARI, Gazipur

Abstract/ Executive Summary:

Multiple shoot proliferation was obtained by culturing shoot tips and nodal explants of jackfruit plants in vitro . The best response towards shoot multiplication was achieved by adding 1.5 mg/1 benzylaminopurine (BAP) in the medium. Microcuttings taken from proliferated shoots produced roots on half woody plant (WP) medium with various concentrations and combinations of IBA and NAA. The optimum rooting was achieved at 2 mg/1 IBA. About 80% of these plantlets survived when rooted plantlets W .1 :1 v/v transferred to the soil substrate.

Keywords: Jackfruit, Micropropagation, Explants

Location:

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Jackfruit

Objectives:

For multiplication of year round jackfruit genotype using in vitro technique

Methodology:

Young leaves, shoot pieces and nodes were collected from 2 to 3 months old seedlings grown in a mist house and then washed with running tap water for about 30 minutes. The explants were then placed in autoclavable jar with 0.1% HgCI2 solution for 10 minutes and washed 4 times with sterile distilled water under clean bench. The shoot pieces and nodes were cut into 1.0 cm long, and leaves were cut into 1.0 cm squares Single explant was cultured in each culture vessel of 30 ml size containing 10 ml woody plant medium (WPM). The culture medium was incubated at 25 ± 20⁰C under fluorescent light (2000 lux) following 16 h day (light) : 8 h night (dark) cycle. The culture medium was composed of woody plant medium with full strength salts , 3% sucrose, 0.8% Difco Bacto agar and different concentrations of cytokinins (BAP, Kinetin) singly or in combination with NAA for shoot proliferation. The pH of the media was adjusted to 5.7 ± 0.1 and the media were autoclaved for 25 minutes at 1210⁰C. The control treatment received no supplements of the growth regulators. Seven to ten days after incubation multiple shoots developed at the base of the explants were subcultured at an interval of 3-4 weeks.Half strength WP salts was used for rooting of shoots . All media were supplemented with 2% sucrose and 0.7% Difco Bacto agar and the pH was adjusted to 5.7 ± 0.1. The rooted plantlets were removed from the culture vessel and agar was carefully washed away and transplanted in small plastic pots containing soil and organic manure mixture (1:1v/v). The potted plantlets were gradually hardened in a mist propagating house through periodic exposures to the low humidity of ex vitro conditions and subsequently established in the soil.

Source of Information: Ann. Bangladesh Agr ic. 10 (I): 85-91. 2000

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Among the three different explants,nodes proved to be the best for multiple shoot proliferation when different concentrations of SAP ( 1.0-5.0 mg/1) and Kn (1 0-5.0 mg/1) were used singly and NAA in combinations with SAP and Kn at 1.0 mg/1. For inducing proliferation and growth of shoots, WP medium containing 1.5 mg/1 SAP was best followed by 1.0 mg/1 SAP in both node and shoot tip. However. the shoot multiplication rate increased with the increasing number of subculture when 1.5 mg/1 SAP was used in WP medium and at about 10 weeks , 4-5 shoots per node culture were produced. For shoot proliferation, kinetm was found less effective than benzylaminopurine NAA in combination with SAP and kinetin inhibited shoot proliferation . It was also observed that shoot proliferation from node and shoot tip explants in WP medium at 5mg/l SAP or Kinetin exhibited no effect. It was also found that callus formation was found at base with higher concentration (5 mg/1) of SAP. Among the auxins tested , rooting response of microcuttings cultured on IBA-containing medium was superior than others However the best response was observed when 2.0 mg/1 IBA was used in Y2 strength WP medium. Both the number and length of roots were increased in IBA treatment. At lower concentration (0.5 mg/1) , IBA and NAA failed to induce root initiation It is interesting to note that the microcuttings produced roots in vitro in the absence of auxins But the number of roots per micro-cutting was few in auxin omitted medium NAA produced roots but all concentrations tended to accelerate callus formation at the micro-cutting base Higher concentration (2-5 mg/1) of NAA. Plant with sufficient roots when transplanted in pots containing a mixture of soil and organic manures (1 1 v/v), about 80% plants survived and established in soil mixture

Knowledge Management: Journal