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Effect of Nitrogen and Coumarin on in Vitro Micro Tuberisation of Potato

M J Hossain
Bangladesh Agricultural Research Institute, Joydebpur, Gazipur-1701, Bangladesh

M A Siddique
Professor
Department of Horticulture, Bangladesh Agricultural University

Abstract/ Executive Summary:

Two in vitro experiments were conducted to test the efficacy of nitrogen and coumarin (an auxin antagonist but auxin-like in activity) on production of microtuber in a popular potato cultivar, Diamant in vitro . In experiment^-1, five levels of N (30, 45, 60, 75 and 90 mM) and two levels of coumarin (0 and 50 mg/l) and in expt. 2, two levels of N (45 and 60 mM) and three levels of coumarin (30, 50 & 70 mg/l) were used. The cultures were incubated for 75, 90 and 105 days in experiment-1 and 90 and 105 days in for experiment-2. Microtuber initiation occurred most early by 5.50 days after culture (DAC) at 30 mM N with 0 mg l^-1 coumarin in experiment-1 against 7.16-8.16 days in experiment-2. N @ 45 and 60 mM produced the maximum of 8.16 microtubers per flask against 6.33 for 30 mM N with 0 mg l^-1 coumarin. The effect of 75 and 90 mM N was the poorest. In experiment-2 the maximum microtuber number was 7.33 at 60 mM N, 30 mg l^-1 coumarin and 105 days after culture, which was statistically similar to 6.83 at 45 mM N, 30 mg l^-1 coumarin and 105 DAC. The highest weight of microtuber per flask was 1217.33 mg at 45 mM N, 50 mg l^-1 coumarin and 105 DAC in experiment-1 and 1003.11 mg at 45 mM N, 30 mg l^-1 coumarin and 105 DAC in experiment-2. The maximum average weight per microtuber was 163.02 mg at 60 mM N, 50 mg l^-1 coumarin and 105 DAC in experiment-1 and 146.41 mg for 45 mM N, 30 mg l^-1 coumarin and 105 DAC in experiment-2. In experiment-1, the maximum effective size microtuber (200 mg plus >100 mg) was obtained from 60 mM N, 50 mg/l coumarin and 105 DAC (72.01%) while it was 81.95% at 45 mM N, 30 mg l^-1 coumarin and 105 DAC in experiment-2. N level of 45 mM with 30 mg l^-1 coumarin was noted as the best for producing potato microtuber within 105 days.

Keywords: Microtuber production, Nitrogen, Coumarin, Duration of culture and Potato

Location: Tuber Crops Research Centre, Bangladesh Agricultural Research Institute, Gazipur

Start Date: 00-00-2006

End Date: 00-00-2006

Program Area: Crop-Soil-Water Management

Commodity: Potato

Objectives:

To develop a medium with modified N levels and coumarin for efficient production of microtubers in relation to culture period.

Methodology:

In vitro ready stock of microplants of the potato cv. Diamant (Dutch origin) was used in this experiment. Two experiments on microtuberisation in relation to nitrogen and coumarin were conducte d at the tissue culture laboratory of Tuber Crops Research Centre, Bangladesh Agricultural Research Institute, Gazipur during 2006. In the first experiment, five levels of nitrogen viz. 30 , 45, 60, 75 and 90 mM and two levels of a growth retardant, coumar in (2H-chromen-2-one) @ 0 and 50 mg l^-1 were tested. Based on results of first experiment, two levels of nitrogen viz. 45 and 60 mM and three levels of coumarin viz. 30, 50 and 70 mg l^-1 were used in the second experiment. Three to five microplants were developed in vitro through liquid shaken culture as described by Espinosa et al. (1986). Microplants were grown on MS culture media as described by Espinosa et al. (1986). At the age of 25 days of the microplants, the liquid culture media were replaced by tuberisation media with the help of 50 ml bottle top dispenser. Tuberisation media contained basic MS (Murashige and Skoog, 1962) salts supplemented with 10 mg l^-1 BA and 8% sucrose. Nitrogen and coumarin were used as per treatments. Forty milliliter of culture media was dispensed in each 250 ml Erlenmeyer flask. The amount of nitrogen (mM) was calculated out from NH₄NO₃, which contains 40.20 mM nitrogen. The cultures were incubated under dark at 19±1⁰C. The experiment was repeated twice.The experiments were conducted following a completely randomized design with three replications. The collected data on microtuber production and grading were properly analysed and the means were separated using LSD test at 5% level of probability level.

Source of Information: SAARC JOURNAL OF AGRICULTURE, Vol. 9, Issue 2, 2011, pp. 17-27, ISSN 1682-8348, SAARC Agricultural Information Centre.

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Findings of the present study reveal that microtuber of potato can effectively be produced using 45 or 60 mM N with 30 mg l^-1 coumarin in media. The maximum percentage (72.01%) of effective size microtuber (>100 mg) can also be obtained with 45 mM N, 30 mg l^-1 coumarin and 105 days duration of culture.

Knowledge Management: Journal