Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Immune Response of Goats to Thermostable Ppr Vaccine in Bangladesh

M. M. Rahman
Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh

A. R. Bhuiyan
Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh

R. Parvin
Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh

M. Giasuddin
Animal Health Research Division, Bangladesh Livestock Research Institute, Savar, Dhaka

M. E. Haque
Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh

S. M. Sayem
Department of Agricultural Statistics, Faculty of Agricultural Economics and Rural Sociology, Bangladesh Agricultural University, Mymensingh

M.R. Islam
Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh

E.H. Chowdhury
Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh

Abstract/ Executive Summary:

To control peste despetits ruminants (PPR) in Bangladesh a live attenuated conventional PPR vaccine was developed by Bangladesh Livestock Research Institute (BLRI) and currently being used in the country. Farmers and field veterinarians often raise the question about the efficacy of that vaccine. The poor efficacy of PPR vaccine in rural situation was thought to be due to break in cold chain system during transportation. To address to this problem, a thermostable preparation of the former PPR vaccine has been made by BLRI. The present study was carried out to assess the efficacy of both the vaccines on the basis of the humoral immune response. A total of 190 goats more than 3 months of age of both sexes were selected for this experiment. Sera collected from experimental goats were analyzed with a competitive enzyme linked immunosorbent assay (cELISA). Percent inhibition (PI) value greater than 50% was considered as positive. In case of conventional PPR vaccine, it was found that 62% of the goats were sero-positive at 21 days post vaccination (DPV), which declined to 34.72 % at 180 DPV. On the other hand, in case of thermostable PPR vaccine, 70% serum samples were found positive at 21 DPV and that decreased slightly to 60% at 180 DPV. Kruskal-Wallis test and paired t- test revealed no significant difference in the antibody response of the thermostable vaccine kept at 25, 30, 35 and 40^oC temperatures for 7 -14 days. peste despetits

Keywords: cELISA, Immune response, Thermostable PPR vaccine, Storage time, Temperature

Location: Livestock Research Institute (LRI), Mohakhali, Dhaka

Start Date:

End Date:

Program Area: Animal Health and Management

Commodity: Goat

Objectives:

To determine the potency in terms of antibody response of newly developed thermostable PPR vaccine kept at gradient temperature of 25⁰, 30⁰, 35⁰ and 40⁰ C for 7 and 14 days as well as immuno-efficacy of conventional PPR vaccine currently being used in Bangladesh.
To assess the efficacy of both the vaccines on the basis of the humoral immune response.

Methodology:

Two types of PPR vaccine, conventional PPR vaccine developed by BLRI and produced by Livestock Research Institute (LRI), Mohakhali, Dhaka and thermostable vaccine experimentally prepared by BLRI, Savar, Dhaka were used in this study. Conventional PPR vaccine was collected from LRI from a freshly prepared batch. Proper cold chain was maintained during transportation of the vaccine. To determine the immunoefficacy of thermostable PPR vaccine, a total of 90 goats over 3 months of ages of both sexes were selected randomly. All the animals were kept under same housing and management conditions at BLRI animal shed. The animals were treated against gastrointestinal helminths with Albendazole at 15 days pre-vaccination. The animals were divided into nine groups comprising of 10 goats each. To maintain storage time and temperature gradients the vaccines were kept in 4 different incubators in Department of Pathology, Bangladesh Agricultural University, Mymensingh and BLRI, Savar, Dhaka. Mean Percent Inhibition (PI) values and standard deviation were determined from the obtained PI values of the serum samples in each vaccinated and control groups. Kruskal-Wallis test we re carried out to compare the immuno-efficiency of vaccine kept at various temperatures and for different time period. Paired t- test was carried out to determine the significance of variation of PI values among the different groups (including control group) at pre and post vaccination. Statistical test were carried out by using the software SPSS Version 11.5 and Minitab 13.

Source of Information: SAARC JOURNAL OF AGRICULTURE, Vol. 9, Issue 2, 2011, pp. 73-81, ISSN 1682-8348, SAARC Agricultural Information Centre.

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

PPR vaccine produced in Bangladesh provided about 70% sero-conversion that declined with time. The reasons of low immunity by PPR vaccine remained unidentified. Immune response of thermostable PPR vaccine kept at different storage temperature gradients and for time being revealed no significant differences indicating that thermosatble preparation of PPR vaccine may be used without maintaining a cold chain.

Knowledge Management: Journal