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Bio Availability and Toxicological Potential of Wheat Bound Residues of Malthioon in Rats

J.A. Khatoon
Departrpent of Biochemistry Bangladesh Agricultural University, Mymensingh-2202 Banqladesh

M H-H.Khan
Departrpent of Biochemistry Bangladesh Agricultural University, Mymensingh-2202 Banqladesh

M.A. Hqssain
D.epartrpent of Biochemistry Bangladesh Agricultural University, Mymensingh-2202 Banqladesh

Abstract/ Executive Summary:

The present study wheat grains were treated with 14C-malathion stored under laboratory condition in glass jar for a maximum period of 180 days and sampled at 1, 15, 30, 60, 90 120 and 180 days for the determination of the insecticide residue. The surface, extractable and bound residues were determined using radio-tracer technique. The amount of bound residues in grains at 10 and 50 ppm gradually increased to 3.8 and 4.2% respectively of the initially applied dose after 180 days. The amount of surface residues has been declined gradually and the values were 32.2% and 33.0% at 10 and 50 ppm, respectively. The amount of extractable residues increased up to 50.9% and 52.0% for applied dose of 10 and 50 ppm, respectively. When rats were allowed to feed these 14C-bound residues, radioactivity excreted in urine counted for 34.5% and in faces 42.5% of the administered doses. In faces, 45.1% was methanol extractable and 54.0% was non-extractable. Maximum bodnd14C-residues as found in fat bodies (3.6%) followed by liver (2.5%), kidney (1.3%), muscle (0.75%), spleen (0.48%), heart (0.30%) and blood (0.10%).

Keywords: Bioavailability,Toxicology, Mal, Wheat

Location: BAU, Mymensingh

Start Date:

End Date:

Program Area: Pest Management

Commodity: Wheat

Objectives:

1.To study the fate and magnitude of malathion residues in 14C-malathion treated wheat in storage

2.To investigate the reduction and distribution of the same.

Methodology:

Chemicals: Malathion (0, 0-dimethyi-S-(1-2-Dithoxy-carbonyl) [1, 2-C] phosphorodithioate), specific gravity 24.8 mci/m mole, radiochemical purity 99% was obtained from International Atomic Energy Agency (IAEA). Non-labeled malathion was also obtained from IAEA. Formulated malathion was obtained from plant protection department of Bangladesh. Grain treatment: Pesficide free wheat grains were collected from Wheat Research Center, Dinajpur. The unwanted materials, broken as well as deformed grains were removed from it. Then the sample was divided into three groups. Sample A was treated with 100 IJCi-malathion and 433.5 mg of 2% non-labeled malathion in order to prepare a final concentration of 10 ppm and sample B was treated with 100 11ci 14C-malathion and 2433.5 mg of 2% non-labeledmalathion to produce 50 PPM whereas sample C was not treated with insecticide and used as control. All the samples were tumbled in separate glass jars for four hours continuously and stored at 22-25°C at relative humidity of 65-75% and moisture content of 10-12%. Sampling and residues determination: Malathion treated and untreated wheat samples were taken at intervals of 15, 30, 60, 90, 120 and 180 days from the storage. 25 g of sample from each group was washed three times with distilled water. Then the washings were collected and analyzed for radioactivity in a Hewlett Packard Liquid Scintillation Counter, Tricarb 460 C (Surface residues). The washed grains were dried, ground thoroughly and extracted with 95% methanol for 24 hours in asoxhlet apparatus. The methanol extract was then counted for radioactivity (methanol extractable residues).

Source of Information: Bangladesh J of Agril. Sci. 30(1): 63-68, January, 2003

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

. The amount of bound residues in grains at 10 and 50 ppm gradually increased to 3.8 and 4.2% respectively of the initially applied dose after 180 days. The amount of surface residues has been declined gradually and the values Vl!ere 32.2% and 33.0% at 10 and 50 ppm, respectively. The amo4nt of extractable resjdues increased up to 50.9% and 52.0% for applied dose of 10 and 50 ppm, respectively. When rats were allowed to feed these 14C-bound residues, radioactivity excreted in urine counted for 34.5% and in faces 42.5% of the administered doses. In faces, 45.1% was methanol extractable and 54.0% was non-extractable. Maximum bpuncj 14C-residues as found in fat bodies (3.6%) followed by liver (2.5%), kidney (1.3%), muscle (0.75%), spleen (0.48%), heart (0.30%) Cjnd blood (0.10%).

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