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Molecular Characterization of Poultry Salmonellae and Production of Formalin Killed Pullorum Disease Vaccine Using Local Isolate in Bangladesh.

Dr. Md. Mokbul Hossain
Associate professor
Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University (BAU), Mymensingh-2202

Dr. Md. Golam Haider
Scientific Officer
Salmonella Section, Livestock Research Institute, Mohakhali, Dhaka-1212

Abstract/ Executive Summary:

Salmonella Pullorum causes pullorum disease resulting severe systematic disease of poultry and that cause economic losses through mortality, morbidity and reducing in egg production. The mortality of pullorum disease confined mainly to the first 2-3 weeks of age chicks and mortality varies from 35 to 100%. The disease also affects layer birds and cocks. For prevention and control of the pullorum disease in chicks potent vaccine is the most important and effective tool to vaccinate layer birds. Salmonella Pullorum bacteria were isolated through different laboratory approaches and the methods were culture in different media, morphological study by Gram’s stain, carbohydrate fermentation test, biochemical test, motility test. One of the isolated bacteria was used as master seed for production of Pullorum disease vaccine. Efficacy of prepared vaccine and molecular characterization of vaccine bacteria are under progress.

Keywords: Molecular characterization, salmonella vaccine, Phytogenetic relationship

Location: BAU, Mymensingh

Start Date: 13-05-2009

End Date: 12-05-2011

Program Area: Pest Management

Commodity:

Objectives:

1) Molecular characterization of the salmonella vaccine developed in Bangladesh that can be used for the patenting of the vaccine. 2) Phylogenetic relationship of the vaccine bacteria with the field isolates will be known.

Methodology:

Sample collection for the mortality of pullorum disease confined mainly to the first 2-3 weeks of age chicks and mortality varies from 35 to 100%. The disease also affects layer birds and cocks. Laboratory experiment of Salmonella Pullorum bacteria were isolated through different laboratory approaches and the methods were culture in different media, morphological study by Gram’s stain, carbohydrate fermentation test, biochemical test, motility test. Data analysis of the isolated bacteria was used as master seed for production of Pullorum disease vaccine.

Source of Information: Bangladesh Agricultural University Research Progress, 07-08 March, 2010. Bangladesh Agricultural Bangladesh Agricultural University Research Progress. Volume 20. Proceedings of the Workshop of University Research System BAURES), BAU, Mymensingh and Annual workshop on BAU research Progress: 2010-2011; BAURES, 02-03 April 2012, BAU, Mymensingh, 02-03-2012

Article Link (Online Journal):

Funding Source:

1.	Budget: 2000000

Total Budget (Tk.) : 2000000

Achievement/Findings:

1) Vaccine from local isolate of Salmonella pullorum has been developed. 2) Molecular method and Polymerase chair reaction (PCR) for diagnosis of Salmonella has been standardized. 3) Efficacy of prepared vaccine and molecular characterization of vaccine bacteria are under progress.

Knowledge Management: Report/Proceedings