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In Vitro Propagation of Banana

M REZAUL KARIM
Biotechnology Divn., BARI, Joydebpur, Gazipur

M . A. MALEK
Plant Genetic Resources Centre, BARl, Joydebpur, Gazipur, 1701

SAJIA RAHMAN
Plant Genetic Resources Centre, BARl, Joydebpur, Gazipur, 1701

M. AL-AMIN
Biotechnology Divn., BARI, Joydebpur, Gazipur

M. RUHUL AMJN
Shere-Bangla Agricultural University, Dhaka-1207, Bangladesh.

Abstract/ Executive Summary:

An in vitro technique for plant regeneration using meristem-derived plantlets of banana cv . BARI-l (Musa sp.) has been developed. Highest number of shoot regeneration was noticed on basal media supplemented with 7.5 mgL-1 BAP + 0.5 mgL-1 NAA at 30 days after inoculation (DAI). The mean number of shoots significantly reduced when the concentrations of BAP and NAA in the medium was high. Regenerated shoots were rooted on half strength MS medillll1 containing 0.5 mgL-1 IAA + 0.5 mgL-1 IBA at 30 DAI. In vitro raised plantlets were transferred to poly bags containing ground soil and cowdung mixture (1:1) for acclimatization and hardening in room temperature (28-30°C) and the established plantlets are ready for planting in the field.

Keywords: In vitro Propagation, Banana (Musa sp.).

Location: Biotechnology Division, Bangladesh Agricultural Research Institute (BARI)

Start Date: 15-08-2008

End Date: 20-07-2009

Program Area: Crop-Soil-Water Management

Commodity: Banana

Objectives:

To study in vitro propagation of banana

Methodology:

The plant materials of banana cv. BARI-l were collected from Biotechnology Division, Bangladesh Agricultural Research Institute (BARI), Joydebpur. Gazipur. The meristem used for establishment of culture was prepared under the microscope from the collected suckers through dissection and removal of leaf sheath. The meristem was obtained from developing suckers (about four months of age) of banana cv. BARI- 1 grown under field conditions and was brought to the preparation room. The suckers were washed thoroughly under running tap water. The roots and outer tissues of the suckers were removed with the help of a sharp knife. A number of outer leaves were removed until the shoot measured about 1.0-2.0 cm in length and 1.0 cm width at the base. The initial explant was prepared under stereomicroscope by removal of outer tissue of meristem with the help of sterile scalpel, which was about 5x5 mm in size and the excised explants were surface sterilized with 0.1% HgC1₂ for 15 minutes and then placed on MS media supplemented with different concentrations of BAP (0.0, 2.5, 5.0, 7.5, and 10.0 mgL-1) and NAA (0.0, 0.5, 1.0, 1.5, and 2.0 mgL-1 ) for in vitro shoot regeneration. The culture tubes with media were autoclaved at 1 .06 kg/cm2 pressure at 121^oc for 25 minutes. The medium was then cooled at room temperature before use. Sterilization of media was done after adding hormone.

Source of Information: Bangladesh J . Agril. Res. 34(2) : 269-278, June 2009 ISSN 0258-7122

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

In vitro raised plantlets were transferred to poly bags containing ground soil and cowdung mixture (I: I) for acclimatization and hardening in room temperature (28-30°C) and the established plantlets are ready for planting in the field.

Knowledge Management: Journal