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Studies on the Genetic Diversity of Pointed Gourd Using Biochemical Methods (isozyme Analysis)

A.S.M.M.R. KHAN
Senior Scientific Officer
On-Farm Research Division, BARI, Joydebpur, Gazipur

M.G. RABBANI
Professor
Department of Horticulture, BAU, Mymensingh, Bangladesh

M.A. SIDDIQUE
Professor
Department of Horticulture, BAU, Mymensingh, Bangladesh

M.A. lSI AM
Professor
Department of Plant Breeding and Genetics, BAU, Mymensingh, Bangladesh

Abstract/ Executive Summary:

Biochemical characterizations of 64 pointed gourds were done using three isozyme viz., acid phosphatase, peroxidase and glutamate oxaloacetate transaminase. A wide range of diversity among the germ plasm based on their acid phosphatase, peroxidase and glutamate oxaloacetate transaminase isoenzyme banding patterns were observed. In respect of isoenzyme activity; 8 acid phosphatase, 7 peroxidase and 9 glutamate oxaloacetate transminase electrophoretic zymotypes were formed by 19, l I, and 19 bands at different Rf values varying from 0.19 to 0.82, 0.38 to 0.69 and 0.15 to 0.95, respectively. The wide range of similarity co-efficient of 0.0-80.0, 0.0-66.0, and 0.0-80.0 as found among the electrophoretic patterns in acid phosphatase, peroxidase, and glutamate oxaloacetate transminase, respectively, indicating wide genetic diversity among the accessions. Based on the polymorphic activity of these three enzymes, 27 combinations of electrophoretic zymotypes were identified, each of which can he equated to genotypes. Each of the groups consisted of one to eight genotypes. Sixty four accessions of pointed gourd were grouped into 12 clusters. The genotypes collected from the same location were grouped into different clusters

Keywords: Genetic diversity, Pointed gourd, Biochemical methods

Location: Horticulture Research Centre, BARI, Joydeppur, Gazipur

Start Date: 00-01-2004

End Date: 00-12-2004

Program Area: Variety and Species

Commodity: Pointed gourd

Objectives:

To know the amount of isozyme variation in pointed gourd and their usefulness to crop improvement programme.

Methodology:

The experiment was conducted with pointed gourd genotypes in polyacrylamide gel system using acid phosphatase, peroxidase, and glutamate oxaloacetate transaminase enzymes. Isozyme analysis was done in the Isozyme laboratory of Horticulture Research Centre, BARI, Joydeppur during the period from January to December 2004. For isoenzyme analysis, young leaf sample of the 64 pointed gourd germplasm were collected and kept under ice before use. One gram of pointed gourd leaf for each genotype was weighted and grounded with small amount of sea s and as a crushing agent with one ml extraction buffer in mortar with pstle. In respect of isoenzyme activity; 8 acid phosphatase, 7 peroxidase and 9 glutamate oxaloacetate transminase electrophoretic zymotypes were formed by 19, l I, and 19 bands at different Rf values varying from 0.19 to 0.82, 0.38 to 0.69 and 0.15 to 0.95, respectively. In zymogram analysis, Rf values were used. Rf values were calculated for each band based upon the migration of the band relative to the front. Rf values=Distance traveled by the band from the tip of the running gel /Distance traveled by the tracking dye.The original data was transformed to Z scores prior to cluster analysis (Anderburg. 1973: Romesburg, 1984). Differences in mobility of enzyme bands were used for zymogram analyses and to find out genetic variability, genetic distance and genetic differentiation of genotype.

Source of Information: Bangladesh J. Agril. Res. 34( I) : 123-141 , March 2009

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The wide range of similarity co-efficient of 0.0-80.0, 0.0-66.0, and 0.0-80.0 were found among the electrophoretic patterns in acid phosphatase, peroxidase, and glutamate oxaloacetate transminase, respectively, indicating wide genetic diversity among the accessions. Based on the polymorphic activity of these three enzymes, 27 combinations of electrophoretic zymotypes were identified, each of which can he equated to genotypes. Each of the groups consisted of one to eight genotypes. Sixty four accessions of pointed gourd were grouped into 12 clusters. The genotypes collected from the same location were grouped into different clusters.

Knowledge Management: Journal