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Effect of Different Levels of Naa on in Vitro Growth and Development of Shoots of Dendrobium Orchid

M.S. PARVIN
Scientific Officer, Plant Breeding Division, BARI,Joydebpur, Gazipur-1701.

M. E. HAQUE
Scientific Officer, TCRC, BARI, Joydebpur, Gazipur-1701.

F. AKHTER
Research Fellow, BAU, Mymensingh-2202, Bangladesh.

F. AKHTER
1& Scientific • Officer, Plant Breeding Division, BARI, Joydebpur, Gazipur-1701, 2Scientific Officer, TCRC, BAR!, Joydebpur., Gazipur-1701, 4Scientific Officer, Biotechnology Division, BAR!, Joydebpur,Gazipur-1701, 3Research Fellow, BAU, Mymensingh-2202, Bangladesh.

MONIRUZZAMAN
Scientific Officer, Biotechnology Division, BARI, Joydebpur,Gazipur-1701.

Abstract/ Executive Summary:

The present study was conducted to investigate the effect of growth regulator NAA on in vitro shoot proliferation, rooting, and plantlet establishment. Among the different concentrations of NAA, the best increase in shoot weight (0.25 g) and shoot number (8.83) were observed from 0.1 mg/I NAA. The highest shoot length (2.60 em), number of leaves (4.83), number of roots (5.15), and root length (2.67 em) were obtained with 0.2 mg!I NAA at 60 DAT.

Keywords: Dendrobium orchid, NAA, MS media.

Location: In the Biotechnology Laboratory, Department of Biotechnology, Bangladesh Agricultural University, Mymensingh

Start Date: 00-07-2006

End Date: 00-11-2006

Program Area: Variety and Species

Commodity: Flower

Objectives:

To study the rooting and acclimatization of Dendrobium plantlet grown in vitro with supplementation of a- Naphthalene acetic acid (NAA) into MS media.

Methodology:

To study the development of shoot, rooting and plantlet establishment, in vitro regenerated 1cm shoot with two leaves of Dendrobium orchid were collected and studied during the period from July to November 2006 in the Biotechnology Laboratory, Department of Biotechnology, Bangladesh Agricultural University, Mymensingh. The experiment was conducted with different concentrations (0, 0.1, and 0.2 mg/1) of hormone supplementation as treatments in MS (Murashige and Skoog, 1962) media to obtain proper growth of shoots and development of roots. Prior to medium preparation, five stock solutions like stock solution of macronutrients, micronutrients, iron source/Fe-EDTA, vitamins/organics and plant growth regulator were prepared.

Source of Information: Bangladesh J. Agril. Res. 34(3): 411-416, September 2009

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Significant variation was found in weight of shoots at different concentrations of NAA at different days after inoculation (OAT). The weight of shoots was found to increase gradually at different OAT, where the highest weight of shoot was obtained from 0.1 mg/L NAA and at all OAI followed by 0.2mg/L NAA and the lowest from control. The present findings on shoot weight is in agreement with Lee et al.(1999) who reported that well developed shoots were found at 0.1 mg/L NAA, and the orchid seedlings died in presence of higher concentration of NAA (1 mg/L).

Knowledge Management: Journal