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Effect of Lemon Grass (cymbopogen Citratus) Extract on Seed Health of Rice

M. Z. Rahman
Senior Scientific Officer
Plant Pathology Division, Bangladesh Agricultural Research Institute, Gazipur 1701

M. Ayub Ali
Professor
Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh 5022

M. S. Hossain
Principal Scientific Officer
Training Division, Bangladesh Rice Research Institute, Gazipur 1701

M. A. Monsur
Senior Scientific Officer,
Plant Pathology Division, Bangladesh Rice Research Institute, Gazipur 1701

M. Z. Zaman
Senior Scientific Officer,
Plant Pathology Division, Bangladesh Agricultural Research Institute, Gazipur 1701

Abstract/ Executive Summary:

Lemon grass extract was evaluated in different dilutions on rice seed health in laboratory and net house experiments. The objectives were to investigate the effect of lemon grass extract on seed-borne pathogen of rice and seedling vigour. Four treatments as 1:1, 1:2, 1:3 dilution of lemon grass extract including a control were studied. Lemon grass extract at 1:1 dilution showed highest germination and vigour index of rice seed and seedlings. It was also found most effective in controlling seed borne fungi of rice. The fungi Bipolaris oryzae, Alternaria padwickii, Curvularia lunata, Fusarium moniliforme, Aspergillus and Penicillium spp. were controlled effectively by soaking seeds in the extract of lemon grass at 1:1 dilution for 6 hours.

Keywords: Lemon grass, Seed health, Rice

Location: Seed Pathology Center (SPC), Bangladesh Agricultural University, Mymensingh

Start Date:

End Date:

Program Area: Pest Management

Commodity: Rice

Objectives:

To determine the effect of lemon grass extract on seed-borne pathogen of rice.

Methodology:

The experiment was conducted in the mycology laboratory and greenhouse of Seed Pathology Center (SPC), Bangladesh Agricultural University, Mymensingh. Leaves of lemon grass were collected, washed with running tap water, dried and then chopped into small pieces. Then the small pieces were ground in a mortar and pastel. The treatments of the experiments were as follows: T_o= control, T₁= 100% , T₂= 50% and T₃= 33% lemon grass extract. To get 100%, 50% and 33% of lemon grass extract, 100, 50 and 33 g lemon grass leaves were crushed in 100 ml water. The crushed materials were filtered through cheesecloth. The extract was kept in conical flask and preserved in a refrigerator at 4±1^oC. Seed samples of BR11 (Mukta) were soaked for 6 hours in grass extracts of respective treatment. Then the plant extract was drained out and the treated seeds were dried on blotter paper. Seed samples (400 seeds for each treatment) were taken to detect the seed- borne pathogen following blotter methods. The petridesh containing seeds were incubated at 25±1^oC for 7 days. After incubation, seeds were examined under stereobinocular microscope for detecting pathogens that grow out of the seeds. Vigour test was carried out using sand in plastic tray (18 x 19). The trays were filled with sterilized moist sand. One hundred seeds were taken randomly from each treatment. Seeds were sown in sterilized sand in each plastic tray in four lines at 25 seeds/ line. A total of 400 seeds were tested for each treatment following ISTA rules (ISTA, 2001) The experiment was laid out in CRD with four replications. Germination was recorded at 7 and 14 days after sowing. Seedlings vigour was calculated as reported by Baki and Anderson (1972). Vigour Index = (mean of root length + mean of shoot length) x percentage of seed germination.

Source of Information: Bangladesh Rice J. 17(1&2): 33-36, 2013

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Lemon grass extract at 1:1 dilution showed highest germination and vigour index of rice seed and seedlings. It was also found most effective in controlling seed borne fungi of rice. The fungi Bipolaris oryzae, Alternaria padwickii, Curvularia lunata, Fusarium moniliforme, Aspergillus and Penicillium spp. were controlled effectively by soaking seeds in the extract of lemon grass at 1:1 dilution for 6 hours.

Knowledge Management: Journal