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In Vitro Propagation of Pointed Gourd (trichosanthes Dioeca Roxb.) Through Encapsulated Shoot Tips

M.A. MALEK
Plant Genetic Resources Centre, BARI, Joydebpur, Gazipur 1701, Bangladesh.

Abstract/ Executive Summary:

Plants were regenerated from encapsulated shoot tips of pointed gourd. Shoot tips isolated from multiple shoot cultures of AM-8 and AM-15 cultivars of pointed gourd were encapsulated in sodium alginate beads. For germination and shoot proliferation, encapsulated shoot tips (artificial seed) were cultured in MS basal medium containing different concentrations and combinations of BAP and NAA. Use of MS medium resulted in 90% conversion of encapsulated shoot tips into plantlets. The results exhibited that BAP and combinations of BAP and NAA play an important role in germination of artificial seed being encapsulated by sodium alginate beads. The plantlets were successfully established in earthen pot. Under the present study, limited experimental efforts have been made to establish the protocol for encapsulating the shoot tips for the production of artificial seed and their subsequent regeneration. It is the first report in Bangladesh in developing artificial seed production technique using vegetative tissue of pointed gourd.

Keywords: Invitor propagation, Pointed gourd, Shoot tips

Location: Bangladesh Agricultural Research Institute , Joydebpur, Gazipur

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Pointed gourd

Objectives:

To develop the avenue for the production of artificial seeds by encapsulating highly regenerative shoot tips of pointed gourd genotypes.

Methodology:

The experiments were conducted in the Tissue Culture Laboratory, Biotechnology Division, Bangladesh Agricultural Research Institute (BARI), Joydebpur, Gazipur 1701. Shoot tips of 3-4 mm long were aseptically excised from in vitro cultured plants of pointed gourd genotypes regenerated by the method described by Maruyama (1996). Sodium alginate beads were produced by encapsulation according to the method of Kinoshita and Saito (1990). The methods involved in this investigation were described under the following separate heads: Methodology comprised of :Culture media preparation,Preparation of Alginate and CaCI₂solution,Media preparation,Alginate solution,CaCI₂solution,Autoclaving and Encapsulation.

Source of Information: Bangladesh J. Agril. Res. 34(4) : 555-563, December 2009

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Use of MS medium resulted in 90% conversion of encapsulated shoot tips into plantlets. The results exhibited that BAP and combinations of BAP and NAA play an important role in germination of artificial seed being encapsulated by sodium alginate beads. The plantlets were successfully established in earthen pot. Under the present study, limited experimental efforts have been made to establish the protocol for encapsulating the shoot tips for the production of artificial seed and their subsequent regeneration. It is the first report in Bangladesh in developing artificial seed production technique using vegetative tissue of pointed gourd.

Knowledge Management: Journal