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Genetic Diversity Analysis in Brassica Varieties Through Rapd Markers

K. GHOSH
Research Fellow, BAU, Mymensingh-2202.

M. E. HAQUE
Scientific officer, Biotechnology Division, BARI. Joydebpur, Gazipur-1701,

Ms. S. PARVIN
Scientific Officer, Plant Breeding Division, BARI,Joydebpur, Gazipur-1701, Babgladesh. 4

F. AKHTER
Scientific Officer, Plant Breeding Division, BARI,Joydebpur, Gazipur-1701, Babgladesh.

M. M. RAHIM
Scientific Officer, Plant Breeding Division, BARI,Joydebpur, Gazipur-1701, Babgladesh. 4

Abstract/ Executive Summary:

This investigation was aimed at exploring the genetic diversity and relationship among nine Brassica varieties, namely BART Sharisha-12, Agrani, Sampad, BINA Sharisha-4, BINA Sharisha-5, BARI Sharisha-13, Daulot, Rai-5, Alboglabra using Random Amplified Polymorphic DNA (RAPD) markers. In total, 59 reproducible DNA bands were generated by four arbitrary selected primers of which 58 (98.03%) bands were proved to be polymorphic. These bands ranged from 212 to 30686 bp in size. The highest proportion of polymorphic loci and gene diversity values were 37.29% and 0.1373. respectively, for BARI Sharisha-12 and the lowest proportion of polymorphic loci and gene diversity values were 8.47% and 0.0318, 8.47% and 0.0382 for BINA Sharisha-4 and Rai -5, respectively. A dendrogram was constructed using unweighted pair group method of arithmetic mean (UPGMA). The result of cluster analysis indicated that the 9 accessions were capable of being classified into 2 major groups. One group consists of BARI Sharisha-12, Agrani, Sampad, Daulot, Rai-5, Alboglabra. where Daulot and Rai-5 showed the lowest genetic distance of 0.049. And another group contains BINA Sharisha-4, BINA Sharisha-5, and BARI Sharisha-1 3, where BINA Sharisha-5 and BARI sharisha-13 showed genetic distance of 0.071.

Keywords: RAPD, Brassica, Genetic distance, Polymorphic band.

Location: Department of Genetics and Plant Breeding of Bangladesh Agricultural University

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Mustard

Objectives:

To provide genetic variation and relatedness of some Brassica varieties by PCR based RAPD technique, as it is important, particularly for selection of diverse parents to be used in hybridization programme.

Methodology:

Nine varieties of Brassica spp. viz., BARI Sharisha-12, Agrany , Sampad , BINA Sharisha-4, BINA Sharisha-5, BARI Sharisha-13, Daulot , Rai-5, Alboglabra were used in the study . Out of the nine varieties, three were BINA varieties (Agrany, BINA Sharisha-4, and BINA Sharisha-5), four BARI varieties (BARI Sharisha-12, BARI Sharisha-13, Daulot, and Rai-5), one was BAU variety (Sampad) and Alboglabra was exotic line from Germany. Seed germination was performed in the laboratory and the germinated seeds were sown in the pot placed in the green house of the Department of Genetics and Plant Breeding of Bangladesh Agricultural University . Fresh and young leaf samples were collected from 12-day old seedlings. Genomic DNA was extracted from young leaf following Phenol: Chloroform: Isoamyl alcohol (25:24: 1 ; v :v :v) purification and ethanol precipitation method. DNA was precipitated with 800 f.tl of absolute ethanol where DNA became visible as white strands by flicking the tube several times with fingers. DNA was pelleted by centrifugation and reprecipitation of the DNA solution was done by adding 400 f.ll of 70% ethanol. The pellets were then air dried and dissolved in 50 f.l1 of TE buffer (IOmM Tris. HC1, 1 mM EDTA , pH=8.0). DNA quality was checked by electrophoresis in 2.5% agarose gel and quantified (Table 1) using a spectrophotometer at 260 nm wave length (Spectronic® GENESIS™). The DNA samples were evaluated both quantitatively and qualitatively using spectrophotometer and agarose gel electrophoresi s, respectively.

Source of Information: Bangladesh J. Agril. Res. 34(3) : 493-503, September 2009

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The highest proportion of polymorphic loci and gene diversity values were 37.29% and 0.1373. respectively, for BARI Sharisha-12 and the lowest proportion of polymorphic loci and gene diversity values were 8.47% and 0.0318, 8.47% and 0.0382 for BINA Sharisha-4 and Rai -5, respectively. A dendrogram was constructed using unweighted pair group method of arithmetic mean (UPGMA). The result of cluster analysis indicated that the 9 accessions were capable of being classified into 2 major groups. One group consists of BARI Sharisha-12, Agrani, Sampad, Daulot, Rai-5, Alboglabra. where Daulot and Rai-5 showed the lowest genetic distance of 0.049. And another group contains BINA Sharisha-4, BINA Sharisha-5, and BARI Sharisha-1 3, where BINA Sharisha-5 and BARI sharisha-13 showed genetic distance of 0.071.

Knowledge Management: Journal