Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

In Vitro Micropropagation of Banana (musa Spp.)

MD. AL-AMIN
Principal Scientific Officer and Head
Biotechnology Division, BARI, Joydebpur, Gazipur 1701

M. R. KARIM
M S Student,
Department of Horticulture and Postharvest Technology, SAU, Dhaka 1207

M. R. AMIN
Professor
Department of Horticulture and Postharvest Technology, SAU, Dhaka 1207

S. RAHMAN
Scientific Officer
Plant Genetic Resources Centre, BARI, Joydebpur, Gazipur 1701

A. N. M. MAMUN
Scientific Officer
Regional Spices Research Centre, BARI, Joydebpur, Gazipur 1701

Abstract/ Executive Summary:

The present study was conducted at the Biotechnology Laboratory, Biotechnology Division, Bangladesh Agricultural Research Institute (BARI), Gazipur during the period from September 2004 to June 2005 to investigate the effect of different concentrations of BAP and NAA on virus free plant regeneration, shoot multiplication and different concentrations of IBA and IAA on in vitro root formation of banana cv. BARI Banana-I. The culture meristem first turned brown in colour in 4-5 days which grew into a green globular hard coat mass after 30-35 days. From this ball like structure, adventitious plantlets were developed . Among the different concentrations, 7.5 mg/1 BAP + 0.5 mg/1 NAA showed highest shoot proliferation of 0.75, 2.75 and 6.25 shoots per explant at 10, 20 and 30 DAI, respectively. The longest shoot (1.03, 2.45 and 3.38 cm) at 10, 20 and 30 DAI, respectively , was produced by the treatment combination of 7.5 mg/1 BAP + 0.5 mg/1 NAA. The maximum number of leaves (2.50, 3.25 and 7.00 leaves/explant at 10, 20 and 30 DAI) were produced on the medium supplemented with the same treatment and it also produced the longest leaves, 0.85, 2.70 and 4.23 em at 10, 20 and 30 DAI, respectively. For root initiation half strength MS medium supplemented with different levels of IBA (0, 0.5, 1 .0 and 1.50 mg/1) and IAA (0, 0.5 and 1.0 mg/1) was used. Root numbers varied with different concentrations of IBA and IAA. The highest number of roots were produced by 0.5 mg/1 IAA + 0.5 mg/1 IBA. The highest length (2.93, 4.63 and 5.88 m) was recorded at 10, 20 and 30 DAI in the same treatment which was statistically significant. Meristem derived plantlets were transferred to poly bags containing 1:1 (ground soil : cowdung) mixture after 7 days hardening in room temperature (28-30⁰C) and established plantlet was ready for planting.

Keywords: Banana, Regeneration, Micropropagation, Plantlet

Location: Biotechnology Division, Bangladesh Agricultural Research Institute (BARI), Gazipur

Start Date: 00-11-2004

End Date: 00-06-2005

Program Area: Variety and Species

Commodity: Banana

Objectives:

i) To study the effect of BAP and NAA growth regulators on in vitro meristem culture (virus free) and shoot proliferation of banana cv. BARI Banana.

ii) To determine the effect of IDA and IAA growth regulators and their concentration required for in vitro root development of banana.

iii) To develop protocol for in vitro rapid propagation of banana.

Methodology:

The present study was carried out in the Biotechnology Laboratory of the Bangladesh Agricultural Research Institute (BARI), Joydebpur, Gazipur-1701, Bangladesh during the period from September 2004 to June 2005. The planting materials of BARI Banana-1 were collected from Biotechnology Division, BARI, Joydebpur, Gazipur. The meristem was obtained from developing suckers of about four months of age grown under field conditions and was brought to the preparation room. The suckers were washed thoroughly under running tap water. The roots and outer tissues of the suckers were removed with the help of a sharp knife. A number of outer leaves were removed until the shoot measured about 1.0-2.0 cm in length and 1.0 cm width at the base. The meristem used for establishment of culture was prepared through dissection and removal of leaf sheath under the microscope. Then the initial explant was prepared under stereomicroscope by removal of outer tissue of meristem with the help of sterile scalpel, which was about 5x5 mm in size. Two experiments were conducted to assess the effect of different concentrations of BAP, NAA, IAA and IDA on shoot proliferation and subsequent rooting of the multiplied shoot. In the frist experiment, in vitro 648 AL-AMIN et al. derived banana cv. BARI Banana-1 plantlets were used as sources of meristem to investigate the effect of BAP, NAA each at different concentrations alone or in combinations on shoot proliferation. Five levels of BAP (0.0, 2.5, 5.0, 7.5, and 10.0 mg/1) and 5 levels of NAA (0.0, 0.5, 1.0, 1.5 and 2.0 mg/1 were used. All the combinations of both BAP and NAA were used as treatments. In the second experiment, there were 3 levels of IAA (0.0, 0.5 and 1.0 mg/1) and 4 levels of IBA (0.0, 0.5, 1.0, and 1.5 mg/1) were used as treatments. The experiments were arranged in completely randomized design (CRD) with 4 replications. Each treatment consisted of 10 culture tubes per replication. Data were collected on the effect of different treatments on shoot proliferation and rooting.

Source of Information: Bangladesh J. Agril. Res. 34(4): 645-659, December 2009

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The culture meristem first turned brown in colour in 4-5 days which grew into a green globular hard coat mass after 30-35 days. From this ball like structure, adventitious plantlets were developed . Among the different concentrations, 7.5 mg/1 BAP + 0.5 mg/1 NAA showed highest shoot proliferation of 0.75, 2.75 and 6.25 shoots per explant at 10, 20 and 30 DAI, respectively. The longest shoot (1.03, 2.45 and 3.38 cm) at 10, 20 and 30 DAI, respectively , was produced by the treatment combination of 7.5 mg/1 BAP + 0.5 mg/1 NAA. The maximum number of leaves (2.50, 3.25 and 7.00 leaves/explant at 10, 20 and 30 DAI) were produced on the medium supplemented with the same treatment and it also produced the longest leaves, 0.85, 2.70 and 4.23 cm at 10, 20 and 30 DAI, respectively. For root initiation half strength MS medium supplemented with different levels of IBA (0, 0.5, 1 .0 and 1.50 mg/1) and IAA (0, 0.5 and 1.0 mg/1) was used. Root numbers varied with different concentrations of IBA and IAA. The highest number of roots were produced by 0.5 mg/1 IAA + 0.5 mg/1 IBA. The highest length (2.93, 4.63 and 5.88 cm) was recorded at 10, 20 and 30 DAI in the same treatment which was statistically significant. Meristem derived plantlets were transferred to poly bags containing 1:1 (ground soil : cowdung) mixture after 7 days hardening in room temperature (28-30⁰C) and established plantlet was ready for planting.

Knowledge Management: Journal