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Evaluation of Spad and Lcc Based Nitrogen Management in Rice (oryza Sativa L.)

M.Sh. lSLAM
Seinor Scientific Officer (Agronomy), BRRI R/S Barisal

M.S. U. BHUIYA
Professor, Dept. of Agronomy, BAU, Mymensingh

S. RAHMAN
Scientific Officer, Plant Physiology Division, BRRI, Joydebpur, Gazipur 1701 and

M. M. HUSSAIN
Chief Scientific Officer & Head , ARD, BRRI, Joydebpur, Gazipur 1701

Abstract/ Executive Summary:

A study was conducted during Boro and T. Aman seasons of 2002 at Bangladesh Rice Research Institute (BRRI), Gazipur to see the relationship of SPAD (Soil plant analysis development) reading with chlorophyll and N contents of leaves and to determine the critical LCC value for rice crops. Hybrid varieties Sonarbangla-1 and BRRI hybrid dhan1 were used for both rice crops and BRRI dhan29 and BRRI dhan31 were used as checks for Boro and T. Aman crops, respectively. Sonarbangla-1, BRRI hybrid dhan 1 and BRRI dhan29 had similar leaf chlorophyll contents in Boro season. The maximum chlorophyll content (1.6-1.8 mg/g leaf) was observed at 39-42 soil plant analysis development (SPAD) value. In T. Aman season, the inbred BRRI dhan31 showed lower amount of chlorophyll (1.2-1.4 mg/g leaf) at 39-42 SPAD value compared to the hybrids Sonarbangla-1 and BRRI hybrid dhan 1. Seasonal variation in chlorophyll content between Sonarbangla-1 and BRRI hybrid dhan1 was not large. Relationship between SPAD value and chlorophyll content was very close (R² =>0.8) at panicle initiation and flower initiation stages for all the varieties. Similar relationship was also observed in case of SPAD value and nitrogen content in leaves. The results indicated that the rice leaves showing higher SPAD readings (>35) had higher chlorophyll and nitrogen contents. The adjusted critical LCC values were 3.0 for Boro and 3.5 for T. Aman seasons for all rice varieties.

Keywords: Chlorophyll, Nitrogen, SPAD, LCC

Location: Bangladesh Rice Research Institute (BRRI), Gazipur

Start Date:

End Date:

Program Area: Crop-Soil-Water Management

Commodity: Rice

Objectives:

To determine relationship between SPAD reading and N and chlorophyll content in rice leaves.
To determine critical threshold LCC value of hybrid and inbred rice varieties.

Methodology:

Field experiments were conducted at the experimental farm of the Bangladesh Rice Research Institute (BRRI), Gazipur during Boro and T. Aman seasons 2002. The experiment was conducted in a randomized complete block design (RCBD) with 3 replications. It was a 2 factor experiments, one factor was N management and the other factor was rice variety. There were 10 N management treatments: N₀= Control (No) N ₁= 40 kg N/ha in 2 equal splits at early tillering (ET) and panicle initiation (PI) stage ,N₂= 80 kg N/ha in 2 equal splits at ET and PI stage, N₃= 120 kg N/ha in 3 equal splits at basal, mid-tillering (MT) and Pl stage ,N₄= 120 kg N/ha in 3 equal splits at ET, MT and PI stage, N₅= 160 kg N/ha in 4 equal splits at basal, MT, PI and heading stage ,N₆= 160 kg N/ha in 4 equal splits at ET, MT, P1 and heading stage, N₇= 30 kg N/ha at 35 Soil Plant Analysis Development (SPAD) value with basal 30 kg N/ha ,N₈= 30 kg N/ha at -^<35 SPAD value (from 20 DAT to heading) without basal N ,N₉= 80 kg N/ha as USG deep placement [3 granules (0.9 g size) in-between alternate 4 hills] Concerning varieties, hybrid Sonarbangla-1 and BRRI hybrid dhan1 were common and inbred varieties BRRI dhan29 and BRRI dhan31 were used in Bora and T. Aman seasons, respectively. The experiment was laid out in a randomized complete block design (RCBD) with three replications. Transplanting was done with 40-day and 25-day old seedlings in Bora and T. Aman season, respectively. One healthy seedling (for both hybrid and inbred varieties) was transplanted in a spacing of 20 x 20 cm. Fertilizers were applied @ 26-41-10-5 kg/ha P, K, S and Zn (blanket dose) as basal before transplanting. Cultural practices for weed and pest management were done as and when necessary. The SPAD and LCC readings were taken from the middle portion of the fully expanded youngest leaves from 5 randomly selected hills. When the SPAD reading came down to critical value 35, corresponding LCC value was considered as critical LCC value of that variety.Chlorophyll was measured from the leaf extract by a spectrophotometer following Yoshida et al. (1976) as: Total chlorophyll (mg/g)= D652 nm x 1000- mg/liter/34.5.

Source of Information: Bangladesh J. Agril. Res. 34(4) : 661-672, December 2009

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

. Sonarbangla-1, BRRI hybrid dhan1 and BRRI dhan29 had similar leaf chlorophyll contents in Boro season. The maximum chlorophyll content (1.6-1.8 mg/g leaf) was observed at 39-42 soil plant analysis development (SPAD) value. In T. Aman season, the inbred BRRI dhan31 showed lower amount of chlorophyll (1.2-1.4 mg/g leaf) at 39-42 SPAD value compared to the hybrids Sonarbangla-1 and BRRI hybrid dhan1. Seasonal variation in chlorophyll content between Sonarbangla-1 and BRRI hybrid dhan1 was not large. Relationship between SPAD value and chlorophyll content was very close (R² =>0.8) at panicle initiation and flower initiation stages for all the varieties. Similar relationship was also observed in case of SPAD value and nitrogen content in leaves. The results indicated that the rice leaves showing higher SPAD readings (>35) had higher chlorophyll and nitrogen contents. The adjusted critical LCC values were 3.0 for Boro and 3.5 for T. Aman seasons for all rice varieties.

Knowledge Management: Journal