Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Screening of Wheat Germplasm for Their Susceptibility Against Different Seedling Diseases

M. U. AHMED
Ph.D. Fellow
Department of Botany, Jahangirnagar University, Savar, Dhaka andPrincipal Scientific Officer, Plant Pathology Division, BARI, Joydebpur, Gazipur 1701

ABUL KHAIR
Professor
Department of Botany, Jahangirnagar University, Savar, Dhaka

I. H. MIAN
Professor
Department of Plant Pathology, BSMRAU, Gazipur 1706

Abstract/ Executive Summary:

Fifteen wheat germplasm, namely BAW-1033, BAW-1045, BAW-1056, BAW1061, BAW-1064, BAW-1004, BAW-1008, BAW-1027, BAW-1035, Sonalika, Gourob, Protiva, Shatabdi, Sourov and Kanchan (check) were screened against different seedling diseases in two consecutive years under inoculated condition. Before sowing, the soils of the experimental field were inoculated with five fungal pathogens, namely Sclerotium rolfsii, Biplaris sorokiniana, Fusarium oxysporum, Pythium aphanid ermatum, and Rhizoctonia solani, Considering the lower percent of diseased seedling and higher vigour index, four wheat germplasrn, namely Shatabdi, BAW-1045, BAW-1004 and Protiva were selected as tolerant materials against seedling diseases.

Keywords: Screening, Wheat germplasm, Seedling diseases

Location: Plant Pathology Division, BARI, Gazipur

Start Date: 00-00-2003

End Date: 00-00-2005

Program Area: Variety and Species

Commodity: Wheat

Objectives:

To find out any resistant/tolerant variety against major seedling diseases of wheat.

Methodology:

A total of 15 germplasm of wheat, namely BAW-1033, BAW-1045, BAW1056, BAW-1061, BAW-1064, BAW-1004, BAW-1008, BAW-1027, BAW1035, Sonalika, Gourob, Protiva, Shatabdi, Sourov and Kanchan (check) were evaluated under pot culture conditions for their susceptibility to different seedling diseases recorded during the survey in wheat fields. The experiment was started with 15 germplasrn of wheat during 2003-2004 growing season and was repeated during 2004-2005 crop seasons using nine germplasm. The earthen pots of 30 cm diameter were filled with sandy loam soil. Before potting, the soil was steam sterilized and mixed with well decomposed cowdung @ 10 t/ha and Urea, TSP. MP, and Gypsum @ 220, 132, 68 and 125 kg/ ha, respectively.Inocula of S. rolfsii, B. sorokiniana, and F. oxysporum were prepared on barley grains. Sterilized barley grains were inoculated with 10 mycelial blocks (0.5 mm diameter) cut from 5 days old PDA culture in petridishes. Inocula of R. solani and P. aphanidermatum were prepared in a mixture of sand : maize meal: water. For multiplication of R. solani, the mixture composed of 20g maize meal: 980g sand:250 ml sterilized water and for P. aphanidermatum maize meal: sand: water were mixed at 3:1:1 ratio. The mixtures were sterilized in autoclave at 121 ⁰C for 15 minuites at 0.7kg/cm² , poured into conical flasks and inoculated with 5 days old mycelial blocks of the fungi. The inoculated flasks were kept under room temperature for 3 weeks for growth of the fungus. The pot soil was inoculated with S. rolfsii, B. sorokiniana, F. oxysporum, P. aphanidermatum and R. solani before 7 days of sowing. The inoculum was mixed at the rate of 100 g/kg of soil.The pots were arranged on the pot yard of Plant Pathology Division, BARI, Gazipur following completely randomized design. After emergence, data on total number of healthy and diseased seedlings in each pot were recorded at every 3 days and continued upto 35 days. Finally percent of diseased seedling was calculated based on total number of seedlings per pot.

Source of Information: Bangladesh J. Agril. Res. 34(4): 673-681, December 2009

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

During 2003-2004 crop season, out of 9 germplasm, BAW-1061, BAW- 1035, BAW-1008, Gourob, Sourob and Kanchan were graded as moderately susceptible (MS), 2 germplasm, BAW -1004 and Protiva were moderately resistant (MR) and remaining 2 germplasm namely BAW-1045 and Shatabdi gave resistant (R) reaction to seedling diseases of wheat. Considering the lower percent of diseased seedling and higher vigour index, four wheat germplasrn, namely Shatabdi, BAW-1045, BAW-1004 and Protiva were selected as tolerant materials against seedling diseases.

Knowledge Management: Journal