M. Ershaduzzaman
SSO
Goat & Sheep Production Research Division, Bangladesh Livestock Research Institute, Savar, Dhaka-1341.
M.J.F.A. Taimur
Bangladesh Livestock Research Institute, Savar, Dhaka-1341
A. K. Saha
Bangladesh Livestock Research Institute, Savar, Dhaka-1341
Dirrhoea, Kid, Sensitivity, Antibiotics
Bacterilogy Lab, Animal Health Research Division of Bangladesh Livestock Research Institute (BLRI), Savar, Dhaka
Animal Health and Management
Performance
A total of 80 diarrhoeic kids from BLRI Goat farm were randomly selected for this study. Faecal samples were collected directly from the rectum using sterile plastic gloves. The samples were send to Bacterilogy Lab, Animal Health Research Division of Bangladesh Livestock Research Institute, Savar, Dhaka and stored in the refrigerator until use. Blood agar (Difco), Nutrient agar (Oxide), MacConkey agar (Difco), Staphylococcus Media No. 110 (Difco), Eosin Methylene Blue agar (Hi- media), Triple Sugar Iron (TSI) agar slant etc. were used for isolation of bacteria from the faecal samples. Nutrient broth (Difco), MacConkey broth (Difco), Tetrathionate broth (Difco) and different sugar media were also used in this study. The different antibiotic discs that were used to determine the sensitivity pattern of isolated bacteria include penicillin, gentamicin, ampicillin, amoxycillin, tetracycline and streptomycin. The concentration of penicillin, ampicillin and amoxycilin was 6 µg, l0µg, 25µg, respectively, whereas for gentamicin, streptomycin and tetracycline the concentration was 10iu, 10iu and 30iu, respectively. Each of the faecal samples were divided and inoculated separately in Nutrient agar and Blood agar to promote the growth of bacteria. Each group of the media were incubated at 37°C for overnight. The colonies on primary cultures were repeatedly subculture by streak plate method until the pure culture with homogenous colonies were obtained. Media, such as Blood agar, Nutrient agar, MacConkey agar, Staphylococcus Media No. 110. Eosine Methylene Blue agar, Triple Sugar Iron agar, Salmonella shigella agar etc. were used for sub-cultures and were incubated at 37°C for 24 hours for growth. A total of 45 day- day old mice of both sexes were used for experimental production of diarrhoea. These mice were divided into six groups (A to F), consisting of 8 mice in each of five (A to E) experimental groups. Remaining one group consisting of 5 mice, was served as control. Four experimental groups of mice were inoculated with individual species of bacteria e.g. Staphylococcus spp. (Gr. A), Bacillus spp. (Gr.B), E. Coli (Gr. C) and Salmonella spp.( Gr.D) and each mouse of group E was inoculated with mixed species of Staphylococcus spp., Bacillus spp., E. Coli and Salmonella spp . Inoculum of each isolate was prepared by culturing the organisms in Nutritrient broth and harvesting 24 hours old culture. The viable counts of the bacterium in these preparations was 5x106 CPU per ml. One drop bacterial suspension (singly and combinedly) was administered orally in each of the mouse of different experimental groups. The mice were observed for every 6 hours interval for 7 days for the manifestation of clinical signs of diarrhoea. Antibiotic in- vitro sensitivity test of faecal bacteria was performed with the standardized commercial sensitivity discs (Sanoft Diagnostics pasture, 9243 Mames-Ia-Coquettes, France) as per method described. Sensitivity to antibiotic was studied mostly on blood agar plates with penicillin 6 µg, streptomycin 10iu, ampicillin 10µg, amoxycilin 25µg, tetracycline 30 iu and gentamicin, 10iu. An amount of 0.5ml freshly grown pure culture of the bacteria was poured on blood agar plates and allowed to spread gently over the entire surface with a glass rod spreader. After 1 to 2 minutes the antibacterial discs were placed on the inoculated plates keeping a distance of about 1 cm apart and incubated at 37°C for overnight. The inhibitory effect of the antibacterial to the growth of the culture was recorded.
Bangladesh Journal of Livestock Research, Vol: 13 (1 & 2), PP: 56-64, 2006, ISSN: 1022-3851
Journal