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Effect of Foliar Application of Chitosan on Growth and Yield in Indian Spinach

M. Monjurul Alam Mondal, PhD
Principal Scientific Officer
Crop Physiology Division, Bangladesh Institute of Nuclear Agriculture, BAU Campus, Mymensingh-2202, Bangladesh

Abstract/ Executive Summary:

A pot experiment was conducted at the pot yard of Bangladesh Institute of Nuclear Agriculture, Mymensingh, during the period from April to June 2011 to investigate the effect of Chitosan application on morphological characters, growth and economic yield in Indian spinach. The experiment comprised five levels of Chitosan concentrations viz., 0 (control), 25, 50, 75 and 100 ppm. The Chitosan was sprayed two times of 15 and 25 days after sowing. Plant height and leaf number plant^-1 increased with increasing concentration of Chitosan whereas branch number, LA, leaf and stem fresh weight as well as total fresh weight increased till 75 ppm followed by a decline. The highest number of branches plant^-1, LA plant^-1, SLW, leaf and stem fresh weight as well as total fresh weight was observed in 75 ppm followed by 100 ppm Chitosan. In contrast, control plants showed the lowest of the above studied parameters. However, the economic parts of Indian spinach, leaf and stem fresh weight as well as total fresh weight was the highest when Chitosan was sprayed @ 75 ppm. These results indicate that application of Chitosan @ 75 ppm is optimum for maximizing plant growth and development of Indian spinach.

Keywords: Indian spinach, Chitosan growth promoter, Vegetable yield

Location: Pot yard, Bangladesh Institute of Nuclear Agriculture, Mymensingh

Start Date: 10-04-2011

End Date: 25-06-2011

Program Area: Crop-Soil-Water Management

Commodity: Indian spinach

Objectives:

Objective of research (Max. 100 words): The research work was undertaken to study the effect of Chitosan on growth, yield attributes and economic yield in Indian spinach under Bangladesh conditions.

Methodology:

A pot experiments was carried out at the pot-yard of Bangladesh Institute of Nuclear Agriculture, Mymensingh, Bangladesh during 10 April to 25 June 2011. The experiment comprised of five concentrations of Chitosan viz., 0, 25, 50, 75 and 100 ppm were applied at 15 and 25 days after sowing. A most commonly local cultivar (green stem and leaves) was undertaken for planting material. The soil was thoroughly mixed with given amounts of urea, triple super phosphate and muriate of potash, gypsum and cowdung at the rate of 10.18, 4.36, 4.27, 2.18 and 150 g pot ^-1corresponding to 200, 110, 100, 40 and 10000 kg ha^-1, respectively. The pots of the experiment were filled with 12 kg of soils. The experiment was laid out with completely randomized design with four replications. Three seeds were sown in each pot on 10 April 2011. Finally, they were thinned to one seedling after 15 days of sowing. The formulation of Chitosan was water-soluble solution. The supplied stock was 25000 ppm. Then it was prepared 25, 50, 75 and 100 ppm for 1L water and spraying was done on Indian spinach plants at afternoon by using a hand sprayer. The first harvest was done at 50 days after sowing (DAS) and second harvest was done at 80 DAS. At each harvest stem was cut at 5 cm above the ground. At each harvest, the morphological, biochemical and yield were recorded. Leaf chlorophyll was determined following the method of Yoshida et al. (1976). Leaf area was measured by automatic leaf area meter (Model: LI 3000 USA). The specific leaf weight was determined as follows: Leaf area ÷ leaf fresh weight. The collected data were analyzed statistically using the computer package programme, MSTAT-C.

Source of Information: Research on Crops 14 (1) : 253-256 (2013)

Article Link (Online Journal):

Funding Source:

1. Government

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Foliar application of Chitosan at early growth stage enhances plant growth and development which resulted increased economic yield in Indian spinach. Among the concentrations, 75 ppm had superiority for plant growth thereby economic yield over 25, 50 and 100 ppm. Therefore, foliar application of Chitosan at 75 ppm may be recommended for Indian spinach cultivation after few more field trials.

Knowledge Management: Journal