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Molecular Characterization and Documentation of Sugarcane Using Ssr Markers

Dr. Md. Amzad Hossain
Chief Scientific Officer
Biotechnology Division, Bangladesh Sugarcane Research Institute, Ishurdi-6620, Pabna

Nadira Islam
Scientific Officer
Biotechnology Division, Bangladesh Sugarcane Research Institute, Ishurdi-6620, Pabna

Kuasha Mahmud
Senior Scientific Officer
Biotechnology Division, Bangladesh Sugarcane Research Institute, Ishurdi-6620, Pabna

Asish Kumar Ghose
Scientific Officer
Biotechnology Division, Bangladesh Sugarcane Research Institute, Ishurdi-6620, Pabna

K M R Karim
Scientific Officer
Breeding Division, BSRI, Ishurdi, Pabna

Mahboob Hussain
Principal Scientific Officer
BJRI Dhaka

Dr. Mohammad Nurul Islam
Professor
Dept. of Botany University of Dhaka

Abstract/ Executive Summary:

This experiment was conducted to establish varietal identification and determine the variation among the sugarcane varieties/clones through DNA Fingerprinting at BSRI, BAU and BJRI Lab during the cropping season 2011-2012. This investigation was conducted using 13 sugarcane varieties such as Isd 16, Isd 18, Isd 20, Isd 28, Isd 33, Isd 34, Isd 35, Isd 36, Isd 37, Isd 38, Isd 39, Isd 40 and BSRI Akh 41. An efficient and easy method was adopted for isolation of high quality DNA from meristem cylinder without liquid nitrogen. The amount of recovered DNA was enough for Polymerase Chain Reaction (PCR) amplification and marker studies such as SSR markers. Electrophorograme of 13 sugarcane varieties using one primer is presented in Figure 4. The results of the present investigation revealed that SSR primer was able to identify the 13 sugarcane varieties.

Keywords: Sugarcane, Molecular markers.

Location: BSRI,BAU and BJRILab

Start Date: 01-07-2011

End Date: 30-06-2012

Program Area: Variety and Species

Commodity: Sugarcane

Objectives:

1.To determine the variation among the sugarcane varieties and active germ plasm collection of BSRI using molecular markers

2..To establish identification of sugarcane varieties and active germ plasm through DNA Fingerprinting.

3.To document sugarcane varieties based on molecular markers.

4.To develop Marker Assisted Selection (MAS) method for sugarcane.

Methodology:

The BSRI-bred varieties, as well as active germ plasm collection preferably flowering germ plasm those collected from home and abroad were used as experimental materials.DNA extraction and purification were followed by the modified mini prep CTAB method. Three individual DNA samples from a cultivar or germ plasm in equal volume and concentration were pooled to form the bulked sample. SSRs primers were used in PCR amplification. Amplified DNA were run in Agarose Gel and Polyacrylamide Gel by electrophoresis to separate allele specific bands. Electrophoresis Gel were documented for Fingerprinting using Gel Documentation System, UV-Transilluminator and Digital Camera as and where required. Documented Electrophoreses Gel were scored on the basis of the presence or absence of the allele specific bands. Data were analyzed to determine the relationships among all cultivars and/or germ plasm collection and cluster analysis were carried out using computer software.

Source of Information: Annual Report 2012-13, BSRI

Article Link (Online Journal):

Funding Source:

1. Government

Budget: 12,50,200.00

Total Budget (Tk.) : 12,50,200.00

Achievement/Findings:

DNA fingerprinting of 13 sugarcane varieties such as Isd 16, Isd 18, Isd 20, Isd 28, Isd 33, Isd 34, Isd 35, Isd 36, Isd 37, Isd 38, Isd 39, Isd 40 and BSRI Akh 41 using SSR primer was performed.

Knowledge Management: Report/Proceedings