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Research Detail

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Dr. Md. Amzad Hossain
Chief Scientific Officer
Biotechnology Division, Bangladesh Sugarcane Research Institute, Ishurdi-6620, Pabna

Kuasha Mahmud
Senior Scientific Officer
Biotechnology Division, Bangladesh Sugarcane Research Institute, Ishurdi-6620, Pabna

Nadira Islam
Scientific Officer
Biotechnology Division, Bangladesh Sugarcane Research Institute, Ishurdi-6620, Pabna

Asish Kumar Ghose
Scientific Officer
Biotechnology Division, Bangladesh Sugarcane Research Institute, Ishurdi-6620, Pabna

Md. Abu Sayem Jiku
Scientific Officer
Biotechnology Division, Bangladesh Sugarcane Research Institute, Ishurdi-6620, Pabna

Dr. Md. Shamsur Rahman
Senior Scientific Officer
Pathology Division, Bangladesh Sugarcane Research Institute, Ishurdi-6620, Pabna

Fifty somaclones of varieties Isd 18, Isd 20, Isd 34, Isd 35, Isd 36, Isd 37 and Isd 38 were inoculated with red rot pathogens in the field. Among the red rot inoculated somaclones, one (Isd 37SC1) showed resistant reaction against red rot disease. Seven somaclones (Isd34SC1, Isd34SC2, Isd35SC2, Isd35SC4, Isd37SC2, Isd37SC3 and Isd37SC5) were moderately resistant and four somaclones (Isd35SC1, Isd35SC3, Isd35SC5, Isd37SC4) were found to be moderately susceptible against red rot disease. DNA fingerprinting of these somaclones was performed using RAPD marker. Among the tested RAPD markers, OPA-02 was the best and showed 58.72% polymorphism. Dendrogram based on linkage distance using Unweighted Pair Group Method of Arithmetic Means (UPGMA) indicated variability among twelve somaclones

  Sugarcane, Somaclone, Red rot, RAPD marker.
  BSRI Lab and Field, Ishurdi
  01-07-2011
  30-06-2012
  Pest Management
  Diseases
  1. To study the effects of red rot toxin, concentrations on different in vitro stages for genetic enhancement.
  1. To study the effects of red rot on regenerated plants under ex vitro conditions for expression of red rot tolerance.
  1. To develop red rot tolerance in sugarcane varieties or clones.

1. BSRI released varieties prone to biotic and abiotic stress were used as experimental materials.

2. Somaclones from defective varieties were produced through callus culture.

3. For callus initiation explants were inoculated on MS + 2,4-D (3-4mg/l) + 10% coconut water.

4. Callus were sub-cultured on the medium containing red rot fungal toxin isolated using standard procedure (5.0, 7.5, 10.0, 12.0, and 15.0%). On every concentration the callus were maintained for 2-3 weeks.

5. The tolerant callus were tested for retention of tolerance by sub-culture first on media without selecting agents, followed by selecting agents containing media.

6. Shoot were regenerated on shooting media (MS+2mg/l BAP + 1mg/l Kn) with and without selecting agents.

7. For root initiation regenerated shoots were sub-cultured on the medium MS + NAA (5mg/l) with and without selecting agents.

8. After hardening, plants were tested under induced red rot under pot and field conditions.

  Annual Report 2012-13, BSRI
  
Funding Source:
1.  Government Budget:  2,00,000.00
   2,00,000.00

Red rot inoculated one somaclone (Isd 37SC1) showed resistant reaction against red rot disease. Seven somaclones (Isd34SC1, Isd34SC2, Isd35SC2, Isd35SC4, Isd37SC2, Isd37SC3 and Isd37SC5) were moderately resistant and four somaclones (Isd35SC1, Isd35SC3, Isd35SC5,   Isd37SC4) were found to be moderately susceptible against red rot disease. DNA fingerprinting of these somaclones was performed using RAPD marker.

  Report/Proceedings
  

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