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Tissue Culture and Utilization of Allied Crops- Date Palm, Palm, Mushroom and Stevia

Nadira Islam
Scientific Officer
Biotechnology Division, Bangladesh Sugarcane Research Institute, Ishurdi-6620, Pabna

Asish Kumar Ghose
Scientific Officer
Biotechnology Division, Bangladesh Sugarcane Research Institute, Ishurdi-6620, Pabna

Kuasha Mahmud
Senior Scientific Officer
Biotechnology Division, Bangladesh Sugarcane Research Institute, Ishurdi-6620, Pabna

Dr. Md. Amzad Hossain
Chief Scientific Officer
Biotechnology Division, Bangladesh Sugarcane Research Institute, Ishurdi-6620, Pabna

Md. Shamsul Arefin
Scientific Officer
Physiology and Sugar Chemistry Division, Bangladesh Sugarcane Research Institute, Ishurdi-6620, Pabna

Md Abul Kalam Azad
Assistant Professor
Dept. of GEB, IU,Kustia

Abstract/ Executive Summary:

This experiment was conducted to find out suitable protocol and sources of explants for micro propagation at BSRI Lab and Field during the cropping season 2011-2012. Two types of explant like shoot tip and meristematic bud of Arabian date palm were used to induce callus. Callus was successfully produced on MS media supplemented with 2, 4-D (5mgl^-1) from both explant. Developed callus was placed on 2, 4-D (44.00 µM) containing MS for shoot regeneration. Callus and shoot were produced successfully. However, root was not produced yet

Keywords: Tissue culture, Stevia, Palm, Date palm

Location: BSRI Lab and Field

Start Date: 01-07-2011

End Date: 30-06-2012

Program Area: Variety and Species

Commodity: Date palm

Objectives:

To identify the suitable sources of explants for micro propagation.
To find out suitable media for micro propagation.
To harden plantlets for transplanting.
To develop tissue culture protocols for micro propagation of Date palm, Palmyra palm and Stevia
To develop low cost method for mushroom seed production through tissue culture.

Methodology:

Shoot tip of Date palm and embryo of Palmyra palm were cultured on MS medium supplemented with different concentrations of cytokinins and auxins for shoot and root production.
Attempts were taken for Mushroom tissue culture by passing laminar-flow-hood and autoclave to produce Mushroom seeds using tissue culture technique.
The explants, shoot tips, leaf base with petiole and nodal segments of Stevia were collected from 4-6 month old yard grown plants.
The explants were cut into small pieces (about 1cm long) and then were treated with 1% savlon for 5-6 minutes with constant shaking and washed thoroughly with distilled water.
Then the explants were taken under laminar airflow cabinet. Surface sterilization were done with a 0.1% mercuric chloride solution containing two-three drops of tween-80 for five minutes under aseptic condition. Surface sterilized explants were washed four times with sterilized distilled water.
Then explants were inoculated aseptically for shooting on MS medium with different concentrations and combinations of growth regulators. Shoots were cultured on rooting media for root production.
Rooted plantlets will be hardened and transplanted in the soil.

Leaves will be collected from the mature Stevia plant. After weighing, the leaves will be washed with distilled water and oven dried at 100 ⁰C for 48 hours. The dry weight of the leaves will be recorded. Dry leaves will be powdered and used in tea for testing sweetness.

Source of Information: Annual Report 2012-13 BSRI

Article Link (Online Journal):

Funding Source:

1. Government

Budget: 2,50,000.00

Total Budget (Tk.) : 2,50,000.00

Achievement/Findings:

Two types of explant like shoot tip and meristematic bud of Arabian date palm were used to induce callus. Callus was successfully produced on MS media supplemented with 2, 4-D (5mgl^-1) from both explant. Developed callus was placed on 2, 4-D (44.00 µM) containing MS for shoot regeneration. Callus and shoot were produced successfully. However, root was not produced yet.

Knowledge Management: Report/Proceedings