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A Study on the Relation Between Total Sperm Count. Motility Percent and Methylene Blue Reduction Time

S. Z . Ali
Dept.of Animal Breeding & Genetics, Bangladesh Agricultural University, Mymenaingh,Bangladeah.

Abstract/ Executive Summary:

100 semen samples of 4 different breeds of bulls were evaluated for total count of sperm per ejaculation, motility percentage and methylene blue reduction time. Simple correlation coefficient between methylene blue reduction time and total count and that between methylene blue reduction time and motility percentage were calculated and a significant negative correlation {r=-0.59) was obtained.

Keywords: Sperm count. Motility%, Methylene blue reduction time

Location: In the artificial insemination Laboratory of Bangladesh Agricultural University, Mymensingh

Start Date: 00-07-1986

End Date: 00-01-1987

Program Area: Variety and Species

Commodity: Cow

Objectives:

To find out relationship between total sperm count, motility percentage and methylene blue reduction time of the semen sample to be inseminated for artificial breeding of cows.

Methodology:

Semen collection was made once a week from each of the 4 bulls belonging to four different breeds namely Sindhi, Sahiwal, Jersey and Holstein Frieslan. The bulls were in regular stud in the artificial insemination Laboratory of Bangladesh Agricultural University, Mymensingh and semen was collected by artificial vagina method according to principle adopted. Immediately after collection of the semen pH, motility percentage and total sperm count were determined. Hydrogen ion concentration was estimated by Nttrazine paper soaked in semen and observing the color change on the Nitrazine paper. The total sperm count was determined by hemocytometer counting chamber method and motility percentage was worked out by actually counting the motile sperm from a small drop of fresh semen on a clean glass slide under microscope at 40X magnification.Methyleneblue reduction test was performed according to method described. Methylene blue solution was prepared first by dissolving 50 mg methylene blue powder in 100 cc of 3.6% sodium citrate buffer ( Na₈C1 H1 072Ha0 ). Then egg yolk citrate extender was prepared by mixing egg yolk, sodium citrate and antibiotics according to Mukherjee and Baner jee (1980). After that 0.2 ml fresh semen was diluted with 0.8 ml of egg yolk citrate diluter in a 10 mIvial and thoroughly mixed. Only 0.1 ml methylene blue solution was then mixed with the former well in the vial. In order to provide an anaerobic condition to the medium the vial was sealed hastily by liquid paraffin layer of 1 em thickness. The solution was than Incubated in a water bath at 4ooc and the time taken by spermatozoa to bleach the solution was noted.

Source of Information: Bangladesh J. Agril. Sci. 20 (1): 11-14: January, 1993, ISSN 0379-4296

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

A total of 100 samples was tested during the experimental period from July 1986 to January 1987 taking 26 samples from each bull of four breeds. The average volume per ejaculation. pH. motility percentage, total sperm count and methylene blue reduction time Sahiwal bull sperm with maximum motile sperm reduced methylene blue dye in shortest possible time.The apparent differences in the above average observations were each subjected to analysis of variance The above analysis of variances Indicate that the differences apparently observed between and among individual dual observation of the bulls were due to chance fluctuation excepting only In the case of motility percentages where a significant difference was noted (P <0.01). The significant negative correlation between total count and reduction time (r--0.59) saw found out and similarly between sperm motility and reduction time. These results are in agreement with Mukherjee and Banerjee (1980) and also with Van Demark and his co-workers (1953). It is therefore important that good quality semen with high motility percentage and total count reduces methylene blue in shortest possible time.

Knowledge Management: Journal