Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Rlbulose-1, 5-bisphosphate Carboxylase I Oxyginase ( Rubjsco) in Rice, as Influenced by Some Growth Regulators

K. Roy
Department of Crop Botany, Bangladesh Agricultural University, Mymensingh.

S. C. Sarker
Department of Crop Botany, Bangladesh Agricultural University, Mymensingh.

M. A. Baten
Department of Crop Botany, Bangladesh Agricultural University, Mymensingh.

Abstract/ Executive Summary:

Ribulose-1, 5-Bisphosphate Carboxylaee/O.xyginase ( RUBISCO ) is the enzyme responsible for photosynthetic CO !•fixation by Ribulose-1,5•Bisphos phate (RUBP) in both C a and Cplants. In rice leaves large subunit of RUBISCO was found to be of about 60-kDa molecular size, when total leaf proteins were fra ctionated by Sodium-Dodecyi-Sulphate•Polyacrylamide-Gei-Eiectrophoresis (50S PAGE), under reducing condition. The enzyme is important becauae its conent and activity determines the photosynthetic efficiency of specially the Ca plants. Rice is Ca in nature. We treated rice seedlings at one months age with various concentrations of Cycocel (CCC); Indole Butyric Acid ( IBA) and Gibberellic Acid (GAa) and measured the amount of.of RUBISCO Content by SDS-PAGE. It was found that all concentration of CCC increased RUBISCO content and the highest content was at 500 ppm. But GA 3 had an opposite effect. Treatment with GAa decreased RUBISCO content at all concentrations. However, application of IBA did not show any significant variation in the content of RUBISCO as com pared to control.

Keywords: Photosynthetic C2 fixation, Phytohormon, Plant growth regulators

Location: Department of Crop Botany,Bangladesh Agricultural University, Mymenslngh

Start Date:

End Date:

Program Area: Crop-Soil-Water Management

Commodity: Rice

Objectives:

To study the effectiveness of various plant growth regulators

Methodology:

In the grill house of the Department of CropLBotany,Bangladesh Agricultural University, Mymenslngh this experiment has been conducted to study the eff ectiveness of various plant growth regulators during May to November 1991. ' Seeds of Oryza fatua were cllected (rom local sources in deep water rice grow ing area of Rajshahi. Seeds of Binas haif and Nazirshail wars obtained from Bangladesh rnstltute of Nuclear Agrlcu lture(BINA). Seedlings were raised from surfact sterilized seejs • and ware grown by pot culture. Design of experiment is completely randomized design. <.rops grown by pot culture were sprayed with various concentrations of chlorocholinchloride (CCC). Indo!e Butyric Acid ( IBA ) and Gibberellic Acid (GA 3) at the age of 30 days of the seedlings. After 15days of the tra.atmant [eaf samples were collected from treated as well as control plants. Total leaf prot,.eins were extracted with protein extraction buffer(8 M urea, 20% glycerol. 4% SDS, 5%2- ME and 125 mM Tris-HCI, PH 6.8) and ware fractionated by SDS-PAGE. RUBISCO was identified by running pure enzyme in the same gel.Sodium-Dodecyi-Sulphate Polyacrylamide-Gel- Electrophoresis-( SDS PAGE): The basic procedure was.that of Laemmli ( 1970 ). Polyacrylamide mini slab gels (10.6 x 6 x 0.1 em) were composed of 2.75% stacking gel (1.5 em) and 15% separating gel (4.5 em). Samples were dissolved in laem mli'a sample buffer at 95°C for 3 min and were separated with a buffer sys tem of 25 mM tris containing 192 mM glycine and 0.1%SDS at room temperat ure. Proteina were run at a constant vol tage of 50 V for 2 hours.

Source of Information: Bangladesh J. Agril. Sci. 20 (1) : 177--181, January, 1993 ISSN 0379-4296

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

In rice leaves large subunit of RUBISCO was found to be of about 60-kDa molecular size, when total leaf proteins were fra ctionated by Sodium-Dodecyi-Sulphate•Polyacrylamide-Gei-Eiectrophoresis (50S PAGE), under reducing condition. The enzyme is important becauae its conent and activity determines the photosynthetic efficiency of specially the Ca plants. Rice is Ca in nature. We treated rice seedlings at one months age with various concentrations of Cycocel (CCC); Indole Butyric Acid ( IBA) and Gibberellic Acid (GAa) and measured the amount of.of RUBISCO Content by SDS-PAGE. It was found that all concentration of CCC increased RUBISCO content and the highest content was at 500 ppm. But GA 3 had an opposite effect. Treatment with GAa decreased RUBISCO content at all concentrations. However, application of IBA did not show any significant variation in the content of RUBISCO as com pared to control.

Knowledge Management: Journal