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In Vitro Regeneration of Aristolochia Tagala Champ- a Rare Medicinal Plant of Chittagong Hill Tracts

Animesh Biswas
Institute of Biological Sciences, University of Rajshahi, Rajshahi, Bangladesh

M A Baril
Institute of Biological Sciences, University of Rajshahi, Rajshahi, Bangladesh

Mohashweta Roy
Institute of Biological Sciences, University of Rajshahi, Rajshahi, Bangladesh

S K Bhadra
Department of Botany, University of Chittagong, Chittagong, Bangladesh

Abstract/ Executive Summary:

An efficient regeneration protocol through in vitro direct organogenesis was developed for a valuable medicinal plant Arist%chia taga/a Champ. using nodal segments as explants. Multiple shoot buds were induced directly from nodal explants cultured on MS (Murashige and Skoog 1962) basal medium supplemented with 2.0 mg/l BAP (N6- benzylaminopurine) and 0.5 mg/l NAA (a-naphthalenacetic acid). The average number of shoots induced per culture was found to be six. Excised shoot roots were cultured on half-strength MS medium containing 0.5 mg/l IBA. The rooted plantlets were transferred to natural environment after proper acclimatization.

Keywords: Aristolochia tagala. Medicinal plant. organogenesis.

Location: Institute of Biological Sciences, University of Rajshahi, Rajshahi, Bangladesh

Start Date:

End Date:

Program Area: Development of Host and Medicinal Plants

Commodity: Medicinal Plants

Objectives:

To vitro propagation of the medicinal plant Aristolochia tagala

Methodology:

Mature seeds of Aristolochia tagala Champ. were collected during field survey in the CHT and sown in earthen pots for raising seedlings. Juvenile twigs from 6-month-old plants were used as source of explants. Surface sterilization of young twigs was done with HgCI₂ solution (0.1 % w/v) for 6-8 min following 5 washes with sterile distilled water. Nodal segments (0.5 cm) of the twigs were cut and cultured on to 8% (w/v) agar gelled MS medium supplemented with various growth regulators (NAA, IAA, IBA, BAP and Kn) at different concentrations and combinations. Sub Culturing was done at 14 - 21-day intervals. Shoot buds were further cultured for elongation in the same medium for two-times at 7-day intervals. Elongated shoot buds were rooted on half strength MS medium fortified with different concentrations of auxins (NAA and IBA) alone. The pH of the medium was adjusted to 5.8 before autoclaving. All cultures were incubated at 25 ± 20 under 16/8h photoperiods. After 12 weeks, plantlets with roots were successfully planted in pot soil through gradual acclimatization.

Source of Information: Journal of Bio- Science. Vol. 15, pp: 63-67, 2007

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

In the present study, it was found that the number of shoot per culture was increased with the number of subculture.Rooting experiments were conducted in 1/2 MS medium supplemented with 0.1-1.0 mg/I either NAA or IBA. Medium containing 0.5 mg/I IBA was proved to be the most effective for rooting of micro shoots than that containing any other concentrations of NAA. In this medium the highest 80% percent shoots induced rooting within two weeks of culture and the mean number of root per culture was 1.17 ± 0.12. The effectiveness of IBA in rooting of in vitro induced shoots of medicinal plants. Shoots with strong and stout root system were acclimatized in outside growth chamber for two weeks for hardening and then transferred to plastic pots placed in natural environment containing mixture of soil and manure (1:1). Eighty per cent plants survived and found normal in natural conditions.

Knowledge Management: Journal