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Comparison Between Single and Double Injection of Pituitary Gland (pg) on the Breeding Performance of Climbing Perch, Anabas Testudineus (bloch)

Shahid Uddin Mahmood
Department of Zoology, University of Dhaka, Dhaka-1000, Bangladesh

Abstract/ Executive Summary:

An investigation was carried out to study the effects of single and double Injection of given dose of pituitary gland (PG) on the breeding performance viz., ovulation response, fertilization, and hatching of climbing perch, Anabas testudineus was tested. Pituitary gland dose of 1.2 mg/ 100 g was used in this experiment. Twenty female fish was arranged into two treatments: T₁and T₂ each with 10 replications. In T₁, the entire dose was administered in a single injection and in T₂ the same dose was given in two equal injections three hr apart. The experiment was laid in completely randomized design (CRD). It was found that administration of PG in split doses (double injection) had no additional advantages (ovulation 100%, fertilization 81.56% and hatching 72.68%) over the single injection (ovulation 100%, fertilization 83.03% and hatching 75.48%) treatment. Similarly, the ovulation time of the fish did not vary significantly in either single or double injection of PG (single injection 7.12 hr; double injection 7.20 hr). The results demonstrated that splitting of pituitary gland dose (double injection) has no additional advantages on breeding performance of A. testudineus. Therefore, single injection may be preferred over the second injection for breeding of this fish.

Keywords: Breeding, Pituitary gland (PG), Fertilization, Hatching

Location: Madina Fisheries Ltd., Dohar, Dhaka

Start Date: 01-05-2000

End Date: 30-06-2001

Program Area: Variety and Species

Commodity: Carp fish

Objectives:

To investigate the effect of administration of a given dose of PG either in single or in double injection on the breeding performance of A. testudineus.

Methodology:

The experiment was conducted in a commercial fish farm, Madina Fisheries Ltd., Dohar, Dhaka in the month of May during the year 2000 and 2001. The brood fishes for this experiment were collected form the hatchery's own brood ponds. Twenty females were divided into two groups and marked as treatment T₁and treatment T₂, having 10 females in each treatment. A constant dose of 1.2mg/ 100 g body weight was used for the experimentation. In T₁the dose was administered at a time (single injection) while in T₂ the dose was divided into two equals (two injections) and administered 3 hour apart. The experiment was laid out following the principle of completely randomized design (CRD). The hormone, PG of carp (Labeo rohita), was obtained from the market of Dhaka City. The fishes were injected at the base of pectoral fin. The injected fishes were allowed to breed naturally in 300 litre metallic trays. To determine the fertilization and hatching rates of individual female fish, a small sample (about 200 eggs) from the spawned eggs of each female was put in plastic bowls (20- litre). After two hours of incubation, percent fertilization was calculated as: percent fertilization = Number of fertilized eggs x 100 total number of eggs (fertilized + unfertilized). The hatching percentage was calculated as: percent hatching = Number of eggs in sample - Dead eggs/ Number of eggs in sample x 100. The ovulation percentage was calculated by the formula: percent ovulation = Number of fish ovulated x 100 total number of fish injected. Water quality parameter during the experiment was measured by a portable test kit. Temperature was measured by a Celsius alcohol thermometer. Water pH was measured by digital pH meter. To analyze the results, the numerical data were saved in the spread sheet module of SPSS 10.0 software. The student's t- test was carried out for the means measurements of fertilization, hatching, and ovulation time using the SPSS statistical package. The null hypothesis that the two treatment means are equal was tested. The t- static was calculated as follows: (M1 - M₂) /S (1/ N₁ + 1/ N₂). Degrees of freedom = N₁ + N₂ - 2. Where M₁ and M₂ are the means of the two treatments, with sample size N₁ and N₂, respectively, S is the pooled estimate of treatment standard deviation.

Source of Information: JOURNAL OF BIO-SCIENCE, THE INSTITUTE OF BIOLOGICAL SCIENCES RAJSHAHI UNIVERSITY, Vol. 14, 2006, pp. 57-60, ISSN 1023–8654.

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The physical and chemical qualities of water in different treatments are presented in this experiment. Water temperature did not differ significantly. Free Breeding Performance of Anabas the results show that splitting of the PG dose (double dose) did not affect the parameters studied (ovulation response, fertilization and hatching rates of eggs, and ovulation time). Both the modes of injection, either single or double, yielded 100% ovulation of the fish. There was no marked difference between fertilization rates obtained from single injection (83.03 ± 3.15%) and double injection (81.56 ± 2.80%) treatments. The difference in means for ovulation time of T₁ and T₂ was not significant. The results suggest that splitting of given PG dose into two injections has no additional advantage on the breeding performances of climbing perch. The differences in fertilization and hatching rates between single and double injection of PG in respect of fertilization and hatching were statistically insignificant. Similarly, no significant effect of single and double injection was found on ovulation time. Therefore, the present observation suggests that any mode (either single or double injection) of PG administration could be applied on climbing perch breeding. However, in case of administration of PG dose in two injections, the brood fishes may get stressed and injured due to excessive handling. Further, it is a labor intensive practice. It would, therefore, be logical to administer PG dose in a single injection. Thus, single injection may be suggested for breeding the fish.

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