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Research Detail

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Md Tamzid Hossain Molla
Pharmaceutical Research Laboratory, Department of Applied Chemistry and Chemical Technology, University of Rajshahi, Bangladesh

M T Alam
Pharmaceutical Research Laboratory, Department of Applied Chemistry and Chemical Technology, University of Rajshahi, Bangladesh

M A-U-lslam
Department of Pharmacy, University of Rajshahi, Bangladesh

Oil was extracted from Terminalia belerica Roxb. seed kernel by solvent extraction process. The whole seed contained 12.28 % oil on dry basis. The physico-chemical properties of the oil were determined. Moisture, ash and crude fibre contents of the seed kernel were found to be 8. 43, 2.54, and 8.78% respectively. The refractive index, co-efficient of viscosity, specific gravity, and energy of activation of the oil were found to be 1.28, 403.6 millipoise at 30?, 0.93 and 6.97 k.cal/mole respectively. The oil was found to be non-drying. Iodine value, acid value, peroxide value, saponification value, saponification equivalent, ester value, unsaponifiable matter, acetyl value, Reichert-Meissel value, Polenske value, free fatty acids as oleic acid and cholesterol content of the oil were recorded as 107, 3.69, 3.14, 189.24, 296.44, 185.55, 1.24%,3.78,0.719,0.945,0.87% and 26.59 mg per 100 g oil, respectively. The oil was qualitative and quantitative analyzed for fatty acid composition by TLC and GLC. The results showed that the fatty acids of the oil had chain length between C14 to C22. The oil contained 17.70% myristic acid, 21.6% palmitic acid, 45.67% oleic acid and 14.93% stearic acid. The whole kernel was analyzed for some nutrients and minerals. The kernel contained 22.57 and 8.38% total lipid and protein respectively. It also contained 0.19mg, 0,45 mg, 0.79g and 1.1 mg of vitamin 81, 82, C and A respectively per 100 g of kernel. Ca, Mg. K, Na, P, Fe, Mn, Zn and Cu were found to be 0.3, 0.02, 0.2, 0.2, 0.01 %; 23, 1, 12 and 12 ppm respectively in kernel and 0.12, 0.05, 1.15, 0.18, 0,45%; 204, 4, 54 and 50 ppm respectively in oil.

  seed oil, kernel, Physicc-chemical behaviour, Picronutrients, Fatty acid profile, GLC.
  University of Rajshahi
  
  
  Quality and Nutrition
  Medicinal Plants

To formulated cereal on albino rats and microbiological investigation of seed oil and seed kernel will form a separate study.

Mature and dried fruits of T. belerica were collected from local market in freshly packed condition and stored in normal room temperature. The seeds were separated from the fruits by scraping the fruit pulp (pericarp) manually. The seeds were dried in the sun for about three days. The sun-dried whole seeds were powdered with a grinding machine (Hammer mill) and the powder was then dried in the oven at 400C for 6 hours and stored in an airtight container for experimental purpose. The powdered materials (seeds) were placed in a thimble of the soxhlet extraction apparatus attached to the mouth of a round bottomed flask containing petroleum ether (B P 40-60?) as an extractive solvent. Some boiling chips were added into the flask to avoid bumping during heating. The extraction of oil was completed in twelve cycles. The petroleum ether extract so obtained was evaporated in a rotary film evaporator (Rotavator) under reduced pressure to obtain oil. The oil obtained was purified with diethyl ether.

  Journal of Bio- Science. Vol. 15, pp: 117-126, 2007
  http://www.banglajol.info/index.php/JBS/article/view/2211/1973
Funding Source:
1.   Budget:  
  

The micronutrients and other related substances in seed kernel along with seed oil were estimated. The results indicated that kernel contained 0.79% vitamin C; 0.19 mg vitamin 81 per 100 g; 0.45 mg vitamin B2 per 100 g, 1104 μg vitamin A (β-carotene) on wet weight basis per 100 g; 22.57% total lipid; and 8.38% protein. The quantity of Ca, Mg, K, Na, P, Fe, Mn, Zn and Cu contents in seed oil and seed kernel were recorded as 0.30% and 0.12%; 0.02% and 0.05%; 0.20% and 1.15%; 0.20% and 0.18%; 0.01 % and 0.45%; 23 ppm and 204 ppm; 1 ppm and 4 ppm; 12.00 ppm and 54.00 ppm and 12 ppm and 50 ppm, respectively. The results cited above revealed that the seed kernel along with seed oil contained in it expresses its high nutrition values. These indicate the important roles these substances must play in the living organism. The foregoing evidences clearly demonstrate that T. belerica seed oil and seed kernel are highly suitable for edible purpose. It is more suitable than other edible seed oils.

  Journal
  


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