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Study of Genetic Variation in Different Hatchery Populations of Common Carp (cyprinus Carpio) of Mymensingh District in Bangladesh Using Microsatlllte DNA Markers

M A S Jewel
Department of Fisheries, Faculty of Agriculture, Rajshahi University, Rajshahi-6205

M M Rahman
Department of Fisheries, Faculty of Agriculture, Rajshahi University, Rajshahi-6205

M N lslam
Department of Biotechnology, Bangladesh Agricultural University, Mymensingh-2202

Abstract/ Executive Summary:

Genetic variation of two strains (Scaled and Mirror) of six hatchery populations of common carp (Cyprinus carpio) was studied using three microsatellite loci (MFW 13, MFW 17 and MFW 28). The loci were amplified by Polymerase Chain Reaction (PCR). The PCR products were electrophoresed on 6% polyacrylamide gel and the alleles were visualized by silver nitrate staining. All the three loci were found polymorphic. The average number of alleles was the highest in the population *SC- SMK (5.00) and lowest in **MC- SMK (4.00). Total number of null allele was more in mirror carp populations (6) than scaled carp populations (4). The average observed heterozygosity (H_o) ranged from 0.54 to 0.67. The average observed heterozygosity (Ho) was lower than the average expected heterozygosity (He) in case of all the populations. The population **SC- BCH had high level of genetic variation in comparison to other five populations because this population had negative 1- Ho/ He values in case of two loci out of three analyzed. The rest five populations had negative 1- Ho/ He values only in one locus. In 6 of a total of 18 tests, significant deviations from Hardy- Weinberg expectations (HWE) were detected. Pair wise Fst values were used to detect the genetic distance between the pairs of populations. The Fst value was highest (0.153) between the population MC- AFF and MC- SMK. Nei's genetic distance value was also highest (0.462) between these populations. The Fst value of 0.153 represents a high level of population differentiation. The lowest Fst value (0.001) was found between SC- SMK and MC- SMK. This value was statistically insignificant.

Keywords: Genetic variation, Common carp, Microsatellite, DNA markers

Location: Mymensingh

Start Date: 01-01-2001

End Date: 30-06-2002

Program Area: Variety and Species

Commodity: Carp fish

Objectives:

To asses the genetic variation among the different hatchery populations of scaled carp and mirror carp and to compare the genetic variability between different populations using microsatellite loci.

Methodology:

Three populations of scale common carp fingerlings and three populations of mirror carp fingerlings were collected from different hatcheries of Mymensingh district viz., as Sagor Matsho Khamar (SMK), Adorsho Matsho Khamar (AMK), Brahmaputra Char Fishery (BCH) and Anil Fish Farm (AFF). Samples of fin tissues were excised and preserved in 95% ethanol and stored at -18 ? . The bands representing particular alleles at the microsatellite loci were scored manually by two persons independently. The bands were designated as a, b, c, d, e and f from the top to the bottom of the gel. The software DNA FRAG version 3.03 was used to estimate allelic length. Allelic variation among the different populations and fit to Hardy-Weinberg proportions were estimated by the software POPGENE with 1000 simulated samples. The software G- stat was used to estimate allelic frequencies and homogeneity test among the different populations. The software FSTAT version 2.9.3 was used to calculate F- statistics (Fst) between populations. The software GDA was used for estimating genetic distance.

Source of Information: JOURNAL OF BIO-SCIENCE, THE INSTITUTE OF BIOLOGICAL SCIENCES RAJSHAHI UNIVERSITY, Vol. 14, 2006, pp. 113-120, ISSN 1023–8654.

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The results of the study showed that the average observed heterozygosity (Ho) was lower than the average expected heterozygosity (He) in case of all the populations. The population **SC- BCH had high level of genetic variation in comparison to other five populations because this population had negative 1- Ho/ He values in case of two loci out of three analyzed. The rest five populations had negative 1- Ho/ He values only in one locus. In 6 of a total of 18 tests, significant deviations from Hardy- Weinberg expectations (HWE) were detected. Pair wise Fst values were used to detect the genetic distance between the pairs of populations. The Fst value was highest (0.153) between the population MC- AFF and MC- SMK. Nei's genetic distance value was also highest (0.462) between these populations. The Fst value of 0.153 represents a high level of population differentiation. The lowest Fst value (0.001) was found between SC- SMK and MC- SMK. This value was statistically insignificant.

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