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Induced Spawning of Puntius Gonionotus (bleeker)

Abdus Salam Bhuiyan
Department of Zoology, University of Rajshahi, Rajshahi-6205, Bangladesh

M Khairul lslam
Department of Fisheries, University of Rajshahi, Rajshahi-6205, Bangladesh

Tanjeena Zaman
Department of Fisheries, University of Rajshahi, Rajshahi-6205, Bangladesh

Abstract/ Executive Summary:

An experiment was conducted to study the induced spawning of Rajputi Puntius gonionotus (Bleeker) in the months of April, May, June and July 2005. Five different doses of PG were used in the experiment. In Puntius gonionotus all the different doses (doses D₁- 3.00 mg/ kg, D₂- 6.00 mg/ kg, D₃- 9.00 mg/ kg, D₄- 12.00 mg/ kg and D₅- 15.00 mg/ kg) were affected equally (100%) in egg release. In the experiment both the males and females were given the 1^stdose but the 2^nd injection was given only to the females after 6 hours. The highest fertilization rates were 70.1 0 ± 2.25% in April (dose D₄), 75.00 ± 2.10% in May (dose D₃), 80.00 ± 2.14% in June and 79.20 ± 2.65% in July (dose D₃). The highest hatching and survivability rates were 61.50 ± 3.1 0 and 55.00 ± 2.13 in April (dose D₄), 68.00 ± 3.5 and 63.00 ± 2.20 in May (dose D₃), 71.80 ± 2.00 and 68.50 ± 2.60 in June (dose D₂), and 70.25 ± 3.50 and 65.70 ± 2.16 in July (dose D₃) respectively. The month of June and the dose D₂ (2 mg in first injection and 4 mg in second injection) were found to be most effective for induced spawning of P. gonionotus.

Keywords: Induced spawning, Hatchling, PG dose

Location: Arappur, Kotwali, Jessore

Start Date: 01-04-2005

End Date: 31-07-2005

Program Area: Animal Health and Management

Commodity: Carp fish

Objectives:

To determine the most effective dose and month with regard to egg release, fertilization, hatching and survival of hatchlings .

Methodology:

The experiments were carried out in hatchery tanks (2 m x 3 m) of the fish seed multiplication farm at Arappur, under Kotwali thana of Jessore district in Bangladesh in cooperation with the farm owner. However some of the analytical works were carried out at the Fisheries Research Laboratory of Zoology Department. University of Rajshahi. The study was carried out during the period from April to July 2005. The hatchery was established in 1985 with a total area of about 10 acres. Mature and healthy Puntius gonionotus of both sexes were collected from local fish farmers as well as from the fish seed multiplication farms, a few months prior to breeding season and reared in a well fertilized (10 kg/ decimal cow dung + 100 g/decimal Urea +200 g/ decimal TSP + 50 g/decimal MP). Stocking pond for spawning. The feeds consisting of fish meal (20%), rice bran (35%), mustard oil cake (22%), wheat bran (16%), molasses (6%) vitamin and mineral premix (1 %), were supplied to the pond every day at the rate of 2% of the body weight of the total stock. The growth and sexual maturity of brood fishes was examined fortnightly. Mature, healthy and uninjured fishes were netted out of the pond and sexed on the basis of sexual dimorphic characteristics. The spawners were acclimatized to the ground water in the holding tank before putting them into spawning tank. The selected breeders were removed from the cistern by hand net and wrapped with well soft cloth. The fishes were injected intramuscularly with different doses of Pituitary gland (PG) between the dorsal fin and lateral line with a 6 ml hypodermic syringe. In this experiment, the first injection was given to both the sexes but the second injection was given only to the females after 6 hours. After hypophysation, the breeders were allowed to breed in the breeding cistern. The ready spawners were caught by net for stripping. The female fishes were stripped and the eggs were collected in an enamel- coated bowl. Milt was pressed out and spread over the eggs and mixed by means of a feather. The eggs were shaken mildly in the bowl and kept for five minutes. In this way the instantly obtained eggs were fertilized by sperms of different time interval viz., instant, 2 minutes, 4 minutes, 6 minutes, 8 minutes and 10 minutes after collection. On the other hand, instantly obtained sperms were used on the eggs of different time interval, viz, instant, 5 minutes, 10 minutes, 15 minutes, 20 minutes, 25 minutes and 30 minutes after collection. The eggs were then washed in tap water and were placed in a hatching funnel (62.8 cm x 43.5 cm). A continuous flow of water was maintained. The hatchlings came out after 24 - 29 hours. The hatchlings were kept in the funnel jar for three days without feeding. After three days the hatchlings were given egg yolk and reared up to sale.

Source of Information: JOURNAL OF BIO-SCIENCE, THE INSTITUTE OF BIOLOGICAL SCIENCES RAJSHAHI UNIVERSITY, Vol. 14, 2006, pp. 121-125, ISSN 1023–8654.

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

In the experiment both the males and females were given the 1^st dose but the 2^nd injection was given only to the females after 6 hours. The highest fertilization rates were 70.1 0 ± 2.25% in April (dose D₄), 75.00 ± 2.10% in May (dose D₃), 80.00 ± 2.14% in June and 79.20 ± 2.65% in July (dose D₃). The highest hatching and survivability rates were 61.50 ± 3.1 0 and 55.00 ± 2.13 in April (dose D₄), 68.00 ± 3.5 and 63.00 ± 2.20 in May (dose D₃), 71.80 ± 2.00 and 68.50 ± 2.60 in June (dose D₂), and 70.25 ± 3.50 and 65.70 ± 2.16 in July (dose D₃) respectively. The month of June and the dose D₂ (2 mg in first injection and 4 mg in second injection) were found to be most effective for induced spawning of P. gonionotus. From the experiment it can be concluded that P. gonionotus can easily be induced and reared, thus it can play an important role in aquaculture.

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