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The Effect of Nitrogen Sources on the Growth of Macrophomina Phaseoli, Botryodiplodla Theobromae and Colletotrichum Corchori

H. U. Ahmed
Principal Sugarcane Pathologist. Sugarcane Research Institute, Iswardi, Pabna, Bangladesh.

M. Huq
Plant Pathology Division, Institute of Nuclear Agriculture, Atomic Energy Centre, Dacca.

Abstract/ Executive Summary:

Eight inorganic sources of nitrogen and seventeen amino acids were taken to study the growth of three jute-pathogens namely, Macrophomina phaseali (Maubl.) Ashby, Botryodiplodia theobromae Pat. and Colletotrichum corchori Ikata and Yoshida. All the three fungi were able to use nitrite, nitrate, ammonium and organic nitrogen. A large number of nitrogen sources supported good growth. M. Phaseoli grew best on proline and glutamic acid, B. theobromae on proline, while C. corchori on calcium nitrate. All of them grew very poorly on the medium without nitrogen, but none of them were able to grow on ammonium carbonate.

Keywords: Effect, Nitrogeh,Growth,Macrophomina

Location: BSRI,Ishurdi, Pubna,

Start Date:

End Date:

Program Area: Pest Management

Commodity: Mite

Objectives:

To study the effect of different nitrogen sources on the growth of three fungi.

Methodology:

M. phaseoli, B. theobromae and C. corchcori were isolated from the diseased jute plants 'The present address is Principal sugarcane Pathologist. Sugarcane Research institute, Iswardi, Pabna, Bangladesh. and they were purified through hyphal tip isolation Eight inorganic sources of nitrogen such as potassium nitrite, potassium nitrate. sodium nitrate, calcium nitrate, ammonium molybdate. ammonium carbonate. ammonium sulphate and urea, and seventeen amino acids namely. glycine. DL- alanine, L-arginine, Dl.-aspartic acid. Lvglu- tamic acid, L-asparagine L tyrosine, L-cys- tine. Lvlysine, LproIine, DL-serine, D'L-thre- onine, L-tryptophane, DL-phenylalanine, DL-methionine. L-leucine and Lshisridin were used in this study. The basal medium constituted Glucose -10_g, K₂HPO₄_,-1.5_g, M_g- SO,. 7H₂0-^O. 5_g, -CaCI₂ 6H₂0. O.l_g, FeSO₄. 7H₂O - 0.007g, MnSO₄, - 0.006g, CuSO₄,. 5H₂0- 0.06g, ZnCl₂ 0.0 75_g, Thiamine-Lmg, Biotin 5 mg and distilled water I liter. The nitrogenous compounds both inorganic and organic were added singly to the basal medium at the rate of 0.425g nitrogen per liter. A treatment without nitrogen was also employed. Cultures were made by taking 40 ml aliquots in 100 ml Erlenmeyer flask and sterilizing in an autoclave at I5 lb. pressure for 15 minutes. The p^H of the medium was adjusted to 6.8 before autoclaving. Seeding of the culture flasks was done by adding one 4 mm diameter mycelial block. The blocks were prepared by means of a sterile cork-borer from the 3 day-old plate cultures of the fungus, grown on 2 per cent water agar medium. The inoculated flasks then kept under room conditions (29 ± 1°C) for growth under stationary condition. After ten days of incubation, fungal mats from the flasks were thoroughly washed with water and filtered using previously weighed filter paper. They were dried to a constant weight at 80^oC in a incubator, kept in a desiccator and then weighed.

Source of Information: Bangladesh J. Agril. Sci. 2 (1) : 25-28 : 1975

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

All the three fungi were able to use nitrite, nitrate, ammonium and organic nitrogen. A large number of nitrogen sources supported good growth. M. Phaseoli grew best on proline and glutamic acid, B. theobromae on proline, while C. corchori on calcium nitrate. All of them grew very poorly on the medium without nitrogen, but none of them were able to grow on ammonium carbonate.

Knowledge Management: Journal