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Embryo Sac Development in Momordica

Sekandar H. Cbowdhury
Assistant Seed Cane Agronomist, Setabgonj Sugar Mill.

Department of Botany Bangladesh Agricultural Univers!ty, Mymensingh.

Abstract/ Executive Summary:

The three species of Momordlca L., namely M. charantia L., M dioica Roxb- and M. convincingness Spreng possess anatropous, bitegmic and crassinucellate ovules. Embryo sac development in all of them is monosporic and 8_nucleate Polygonum type. Of tho four linear megaspores, the chalazal one is always functional. The mature embryo sac of M. cochinchinensls shows a sharp attenuation at its two ends, and it is also much larger than those of tho other two species

Keywords: Embryosac,Development,Momordica

Location:

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Vegetables

Objectives:

To investigate the nature of embryo sac development in the three species.

Methodology:

The flower buds of M. charantia, M. dioica and M. cochinchinensis, at their different stage of development, were collected and only their ovaries were taken. These were then fixed in Formalin .Acetic- Ethyl Alcohol for a minimum period of 24 hours. In M. cochinch;nensis, where the ovaries were large and massive, most part of the ovarian wall was trimmed off in order to facilitate quick fixation, dehydra- tion and easy infiltration of paraffin. The material was subsequently dehydrated in ethyl alcohol -xylene series, infiltrated with paraffin and finally paraffin blocks in the usual way. In order to obtain serial longitudinal sections of the ovules, the ovaries embedded in paraffin blocks were sectioned transversely at a thickness of 7-15 microns with the help of a rotary microtome. Sections of M. charantia were stained with Safrunin-Fast green combination (Sass, 1951). Sbarman's ( 1943 ) schedule (modified), in which he recommended the use of Safranin ° and Orange G in combination with tan- nic acid, gave better results in staining the sections of the other two species. Permanent slides were made in the usual manner.

Source of Information: Bangladesh J. Agril, Sci. 4 (2) : 121-125 : 1977

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Embryo sac development in all of them is monosporic and 8_nucleate Polygonum type. Of tho four linear megaspores, the chalazal one is always functional. The mature embryo sac of M. cochinchinensls shows a sharp attenuation at its two ends, and it is also much larger than those of tho other two species

Knowledge Management: Journal