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Bioassay of Diazinon and Metasystox-r on Jute Hairy Caterpillar. Diacrlsia Obllqua Walker (lepidoptera: Arctiidae)

M. Shahjahan
Department of Entomology, Bangladesh Agricultural University, Mymensingh

Bhuiyan N. Islam
Department of Entomology, Bangladesh Agricultural University, Mymensingh

Abstract/ Executive Summary:

Bioassay studies of the technical grades cf Diazinon and Metasystox-R (Organo-phosphorus insecticiles) were done with a view to evaluating their lethal toxicity on various developmental stages of jute hairy caterpillars, Diacrisia obliqua Walker under laboratory condition. The relative toxicity of these chemicals was also assessed both as contact and stomach poisons.

Keywords: Bioassay, Diazinon, Hairy Caterpillar

Location: Department of Entomology, Bangladesb Agricultural University, Mymensingh

Start Date:

End Date:

Program Area: Pest Management

Commodity: Insects

Objectives:

To evaluate their lethal toxicity on various developmental stages of jute hairy caterpillars, Diacrisia obliqua Walker under laboratory condition. The relative toxicity of these chemicals was also assessed both as contact and stomach poisons.

Methodology:

Fresh groundnut leaves of equal quantity were dipped into various concentrations of Diazinon 98.7% E. C. and Metasystox-R 93.9% E. C. (Technical grades) ranging from 125 ppm to 750 ppm for 2-3 minutes and the leaves were allowed to dry for 5 minutes. Twelve caterpillars of equal age from 3rd, 4th, 5th, and 6th instars were fed separately on treated leaves in jelly jars and the mouth of the jelly jar was covered with piece of cloth and rubber band, . Each treatment was replicated 5 times along with the controls. Larval mortality was recorded separately for 12,24,36, and 48 hours after release. Similarly, an equal number ot larvae from 3rd, 4th, 5th and 6th instars were allowed to feed on fresh groundnut leaves spread over the blotting papers of uniform size treated with various concentrations of Diazinon and Metasystox-R (Technical grades) in jelly jars. Blotting papers were uniformly sprayed with different concentrations (ranging from 125 ppm to 750 ppm) of the candidate compounds by a standard hand atomizer of 100 ml capacity and dried for 5 minutes. Fresh groundnut leaves of equal number were placed over the treated blotting papers and larvae were released in batches of 12 per treatment. Each treatment was replicated 5 times from different instars along with the controls and the observations were made on the larval mortality at various concentrations and hours after release. The amount of insecticides absorbed by the leaves or by blotting paper were not accounted for as the concentrations of the solution were known. The mortality date were corrected by Abbot's formula and the data obtained after 24 hours of treatment were subjected to probit analysis separately for each instar. The experiment was conducted at a room temperature of 28.64⁰±0.07.

Source of Information: Bangladesh J. Agril. Sci. 1 (2) : 135-139; 1974

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Relative toxicity of the insecticides decreased gradually with the increase of larval ages. In all cases Diazinon as stomach and contaet poisons was superior to Metasystox-R, although their effectiveness varied in different development stages of Diacrisia obliqua Walker.

Knowledge Management: Journal