Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Virus Free Stock Production of Some Indigenous Potato Varieties of Bangladesh

M. S. Islam
Department of Horticulture, Institute of Postgraduate Studies in Agriculture, Salna, Gazipur-1703, Bangladesh

A. R. Chowdhury
Department of Horticulture, Institute of Postgraduate Studies in Agriculture, Salna, Gazipur-1703, Bangladesh

Abstract/ Executive Summary:

Out of three (24,30 and 35°C) heat treatments, given to in vitro plantlets followed by meristem culture, 35°C was the best for all the varieties. The percentage of virus free plantlets was higher in exotic varieties than the indigenous at all levels of incubation temperature of in vitro plantlets.

Keywords: Potato, Exotic, Incubation, Indigenous, In vitro, Plantlets

Location: Tissue Culture Laboratory of the Institute of Postgraduate Studies in Agriculture (IPSA), Salna, Gazipur, Bangladesh.

Start Date: 00-11-1992

End Date: 00-05-1993

Program Area: Variety and Species

Commodity: Potato

Objectives:

To produce virus free stock of some indigenous potato varieties of Bangladesh by the combination of heat treatment followed by meristem culture.

Methodology:

The experiment was carried out in the Tissue Culture Laboratory of the Institute of Postgraduate Studies in Agriculture (IPSA), Salna, Gazipur, Bangladesh during the period from November 1992 to May 1993. Four indigenous varieties (Ausha, Challisha, Lal Pakri and Sada Gutti) and the two exotic varieties (Cardinal and Kufri Sindhuri) were used in this study. The indigenous varieties were collected from the Department of Horticulture, Bangladesh Agricultural University, Mymensingh and the two exotic varieties were collected from the farmers field of BADC seed potato growing area of Kashimpur zone, Gazipur. The presence of PLRV, PYX, PVY, PVM and PVS were detected in these varieties. The virus indexed potato varieties were grown in a net house. One month after emergence, one hundred shoot tips of each variety were collected. The shoot tips were first sterilized in 70% ethanol for 30 seconds, stirring with magnetic stirrer followed by washing in sterile water three times. The shoot tips were then sterilized by stirring for 15 min in 0.5% sodium hypochlorite solution containing two to three drops of Tween-20. The explants were then rinsed three times with sterile water. One hundred and fifty single nodes of each variety were excised from sterilized plant samples and placed in test tubes containing 10 ml of MS medium solidified with 2.0 g/l of gelrite. Cultured nodes developed into plantlets in the test tubes and these were subcultured for one time seven days prior to heat treatment. The in vitro produced shoots as mentioned above were grouped into three sets for heat treatment. Each set contained 30 plantlets of each variety. Of the three sets of in vitro plantlets, one set was incubated at 35°C, another set at 30°C and the rest was incubated at 24°C, the latter serving as control. The incubation period varied from three to seven weeks. One hundred and fifty single nodes of each variety were excised from sterilized plant samples and placed in test tubes containing 10 ml of MS medium solidified with 2.0 g/l of gelrite. Cultured nodes developed into plantlets in the test tubes and these were subcultured for one time seven days prior to heat treatment. The in vitro produced shoots as mentioned above were grouped into three sets for heat treatment. Each set contained 30 plantlets of each variety. Of the three sets of in vitro plantlets, one set was incubated at 35°C, another set at 30°C and the rest was incubated at 24°C, the latter serving as control. The incubation period varied from three to seven weeks. Meristem were excised when the foliage showed severe chlorosis or after seven weeks, whichever came sooner as mentioned. Dome-shaped meristem with a two-leaf primodia were dissected out by a heat sterilized blade under a dissecting microscope (20 - 40x) inside the dean bench. Thirty meristem from each set of in vitro heat-treated plantlets of each variety were excised. Immediately after dissection the meristem were placed in MS medium (10 ml in each test tube) supplemented with 0.01 mg/l of NAA and 1 mg/l of kinetin. The medium was solidified with 2.0 g/l of gelrite and the pH was adjusted to 5.8 before autodaving, which was carried out at 121°C for 15 min. Cultures were incubated at 25°C constant temperature under 16 h photoperiod. The meristems grew into plantlets. The in vitro plantlets regenerated from dissected meristems were subcultured after 60 days of incubation for multiplication by single node cuttings. At that time the regenerated plantlets became 3 - 4 cm in length. For subculturing of regenerated plantlets, MS medium at full strength was supplemented with nicotinic acid (0.5 mg/l), pantothenic acid (2.5 mg/l), pyridoxin (1.0 mg/l), thiamine HCl (0.5 mg/l), myoinositol (100.0 mg/l), sucrose (30.0 g/l) and solidified with gelrite (2.0 g/l). The condition of culture was similar to meristem culture. The detection of virus free plantlets was done through ELISA test as well as by EM by taking leaves from the heat treated meristem-derived cultures.

Source of Information: Plant Tissue Culture, Vol: 8, (1), pp: 41-47, (June) 1998

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

The maximum percentage of virus free plants in all the infected potato varieties at 35° C incubation temperature of in vitro plantlets followed by meristem culture indicates the last-mentioned temperature to be the most effective for virus eradication.

Knowledge Management: Journal