Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

In Vitro Propagation of Holarrhena Antidysenterica,wall.wedelia Chinensis(osb).merr. and Woodfordia Fruticosa

S. A. M Nurul Islam
Research Officer
Silviculture Genetics Division, Bangladesh Forest Research Institute, P.O. Box- 273,Chittagong.

Abstract/ Executive Summary:

In vitro multiple and axillary shoots were induced from apical shoot tips and nodal buds of Holarrhena antidysenterica, Wedelia chinensis, and Woodfordia fruticosa in MS supplemented with BAP (0.5-3.0 mg/l and kn (0.5-1.0 mg/l). GA₃ was suitable for elongation of shoot and healthy shoots in Woodfordia fruticosa. Huge multiple shoots with aerial tiny roots were produced in the culture medium containing BAP.Excised shoots of Wedelia chinensis ,and Woodfordia fruticosa rooted when they were cultured in half strength of MS containing IBA 1.0 mg/l but Holarrhena antidysenterica produced roots only when medium was supplemented with NAA (1.0-3.0 mg/l).The rooted shoots of these species were successfully acclimatized and established in the soil.They were vigorously growing in nature. Wedelia chinensis, and Woodfordia fruticosa produced profuse branching and flowered in nursery within 12-15 weeks.

Keywords: Medicinal plant, Holarrhena antidysenterica ,Woodfordia fruticosa, Wedelia chinensis. In Vitro cloning, Multiple shoot.

Location: Tissue culture laboratory, Genetics Division, Bangladesh Forest Research Institute, Chittagong.

Start Date: 01-07-2005

End Date: 31-06-2008

Program Area: Variety and Species

Commodity: Akash moni

Objectives:

a) To develop techniques for rapid regeneration of Holarrhena antidysenterica, Wedelia chinensis and Woodfordia fruticosa species.

b) To develop an easy methods of micro propagation of medicinal plants.

Methodology:

Young growing shoots of 3 medicinal plant Holarrhena antidysenterica, Wedelia chinensis and Woodfordia fruticosa species. were collected from adult plants.These were cut into small pieces (5-10 mm long) and used as explants.They were sterilized with 0.1% (w/v) mercuric chloride in water solution for ten minutes and then washed with autoclaved distilled water. The explants were inoculated in MS supplemented with BAP (6-Benzyl Amino Purine) or NAA (?-Naphthalene Acetic Acid). Then BAP, Kn (Kinetin) and GA₃(Gibberellic Acid) were supplanted in MS for multiple shoots induction and elongation. All the cultures media were gelled with agar (7 g/l) and pH was adjusted at 5.8 before autoclaving. Growth room was maintained at 27±2º C temperature with a photoperiod of 16 hours. Half strength of MS supplemented with IBA (Indole Butyric Acid) and NAA were used for root induction. Rooted plant lets thoroughly washed under running tap water and kept in culture tube in water for two-three days in the growth room.Then the plant lets were transferred in soil (soil filled pots and nursery beds maintaining high humidity) in the nursery . Initiation of multiple shoot and root production on excised shoots have been tried. But success has come only in 3 species viz.( Kurchii (Holarrhena antidysenterica), Dhaiphul (Woodfordia fruticosa, and Kesaraj (Wedelia chinensis) and rooted plant lets were transferred in propagation bed/bag for multiplication.

Source of Information: Bangladesh Association for Plant Tissue Culture & Biotechnology (BAPTC&B).19(2):253-255, 2009

Article Link (Online Journal):

Funding Source:

Budget:

Total Budget (Tk.) :

Achievement/Findings:

Cloning of genetically superior medicinal plants and production of unique huge plant lets through micro propagation could be mitigated the future demand. The large number of improved planting stock of 3 medicinal plants viz. Holarrhena antidysenterica, Wedelia chinensis,and Woodfordia fruticosa have been produced through tissue culture technique within short time which is being used for rapid regeneration and raising quality plantation.

Knowledge Management: Journal