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Microbial Occurrence at Different Freezing Duration in Speckled Shrimp, Metapenaeus Monoceros (fabricius, 1798) from Local Markets of Dhaka City

MD. ANISUR RAHMAN KHAN
Department of Fisheries, University of Dhaka, Dhaka-1000, Bangladesh.

TAMANNA YASMIN
Department of Fisheries, University of Dhaka, Dhaka-1000, Bangladesh.

SA!MA SHARIF NILLA
Department of Fisheries, University of Dhaka, Dhaka-1000, Bangladesh.

Mo. MAHMUDUR RAHMAN KHAN
Department of Fisheries, University of Dhaka, Dhaka-1000, Bangladesh.

MD. MAHMUDUR RAHMAN KHAN
Department of Fisheries, University of Dhaka, Dhaka-1000, Bangladesh

Abstract/ Executive Summary:

A study was carried out at four different (0, 1, 7 and 30 days) freezing durations in regard to the microbiological incidence in speckled shrimp (M etapenaeus monoceros) from three different local markets - Ananda bazaar, New Market and Polashi bazaar of Dhaka city. The study unveiled that Ananda bazaar samples were more or less contaminated than those of others. The microbial load varied with market sources as total bacteria count (TBC), total coliform count (TCC) and faecal coliform count (FCC) were found in all the storage periods crossing the acceptable limit. Except for E. coli (EC), all the bacterial counts were significantly different (p < 0.05) in 7 and 30 days freezing durations. A significant difference (p < 0.05) was found in all freezing durations only for EC counts. After 30 days storage duration for all counts showed 101 cfu/g reduction of microbial load from every initial count. The Salmonella-Shigella (SS) and Vibrio spp. were identified in all the samples of the three markets. In case of biochemical composition, an inverse relation was found in moisture - protein and moisture - fat where the moisture content increased after 30 days freezing duration but protein and lipid contents decreased with time. This study revealed that shrimp available in local markets of Dhaka city was highly ingested with pathogenic bacteria which indicate the unhygienic condition of the market premises.

Keywords: Microbial load, Freezing duration, Metapenaeus monoceros, Food safety

Location: Ananda bazaar, New Market and Polashi bazaar of Dhaka city.

Start Date: 00-01-2011

End Date: 00-07-2011

Program Area: Postharvest and Agro-processing

Commodity: Shrimp

Objectives:

To estimate the harmful microbial load as well as nutritional quality in locally available speckled shrimp .

Methodology:

Sample collection and identification: Fresh speckled shrimp (Metapenaeus monoceros, locally known as horina chingri) samples were collected from three different local retail markets - Ananda bazaar, New Market and Polashi bazaar of Dhaka city in between January to July, 2011. The fish samples were collected by special sterile ziplock bags to avoid further contamination and transported in an insulated box(¹⁰) to the laboratory.. Sample preparation: The samples were processed and used within 2 hours of collection. The whole fish body was considered and thawed at room temperature to assess the microbial load following the method of APHA(¹²) The samples (2.5 g) were separately homogenized with normal saline solution and prepared using 1 - 0.1 ml of the treated samples.Microbial analysis: Spread plate method was used to enumerate total bacterial count (TBC) using nutrient agar (NA) medium and serially diluted samples were pour-plated on MacConkey and membrane faecal coliform (mFC) agar for total coliform count (TC) and faecal coliform count (FC), respectively(¹³) The plates were incubated for 24 hrs at 35 - 37°C for TBC and at 44.5°C for 24 hrs for TC and FC. Further confirmation was done by growing in eosin methylene blue (EMB) agar and calculated as cfu/g. A loopful of growth from the broth was streaked on Salmonella-Shigella agar (SSA), incubated for 48 hrs at 35 - 37°C. Xylose lysine deoxycholate (XLD) agar also used for the selective isolation of Shigella and Salmonella spp. Diluted homogenates were pour-plated on thiosulphate citrate bile sucrose (TCBS) agar to enumerate Vibrio spp. All cultured pathogens were identified by biochemical tests. Biochemical tests: Biochemical tests were done according to the manual for general bacteriology of the American Society of Microbiology(¹⁴⁾. Oxidase, catalase, carbohydrate fermentation/utilization, Kligler's iron agar (KIA), indole production, methyl red (MR), Voges-Proskauer (VP), citrate utilization, nitrate reduction, motility indole urea (MIU), and salt tolerance (3, 5, 10 and 15% NaCl) tests were done to identify the bacteria(¹⁵). Proximate composition analysis: Proximate analysis such as moisture, protein, lipid and ash were carried out following the methods of AOAC(¹⁶) Statistical analysis: Statistical analysis was performed with the SPSS software package (version 11.5) following Tukey' s HSD post hoc for the multiple comparisons.

Source of Information: Dhaka Univ. J. Biol. Sci. 22(2): 91-101, 2013 (July)

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The study unveiledor disclosed that Ananda bazaar samples were more or less contaminated than those of others. The microbial load varied with market sources as total bacteria count (TBC), total coliform count (TCC) and faecal coliform count (FCC) were found in all the storage periods crossing the acceptable limit. Except for E. coli (EC), all the bacterial counts were significantly different (p < 0.05) in 7 and 30 days freezing durations. A significant difference (p < 0.05) was found in all freezing durations only for EC counts. After 30 days storage duration for all counts showed 10¹ cfu/g reduction of microbial load from every initial count. The Salmonella-Shigella (SS) and Vibrio spp. were identified in all the samples of the three markets. In case of biochemical composition, an inverse relation was found in moisture - protein and moisture - fat where the moisture content increased after 30 days freezing duration but protein and lipid contents decreased with time. This study revealed that shrimp available in local markets of Dhaka city was highly ingested with pathogenic bacteria which indicate the unhygienic condition of the market premises.

Knowledge Management: Journal