Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

In Vitro Flowering of Two Epiphytic Dendrobium Species

S. K. BHADRA
Department of Botany, University of Chittagong, Chittagong

T. K. BHOWMIK
Department of Botany, University of Chittagong, Chittagong

Abstract/ Executive Summary:

Four to six months old in vitro grown seedlings of Dendrobium lindleyi and D. tortile having four to six leaves were aseptically grown on 0.8% (w/v) agar solidified MS and MVW media supplemented with different combinations of BAP, picloram, NAA, Kn and IAA at different concentrations. D. lindleyi flowered on MS and MVW fortified with 2.0 mg/I BAP + 0.5 mg/I picloram and 3.0 mg/I BAP + 1.0 mg/I NAA + 1.5 mg/I Kn, respectively . D. tortile flowered on MS medium supplemented with 3.0 mg/I BAP + 1.0 mg/I NAA + 1.5 mg/I Kn or with 2.0 mg/I BAP and 0.1 mg/I IAA.

Keywords: Dendrobium lindley, D. tortile, in vitro flowering

Location:

Start Date:

End Date:

Program Area: Resource Development and Management

Commodity:

Objectives:

To know the potentiality of in vitro technique for the production of Dendrobium lindleyi and D. tortile

Methodology:

Four to six months old in vitro grown seedlings (2 - 3 cm in height) of Dendrobium lindleyi and D. tortile were aseptically grown on 0.8% (w/v) agar solidified MS and MVW media supplemented with different combinations of plant growth regulators (PGRs) and activated charcoal (AC): (i) 2.0 mg/I BAP + 0.1 mg/I picloram, (ii ) 2.0 mg/I BAP + 2.0 mg/I NAA, (iii ) 2.5 mg/I BAP + 0.1% (w/v) AC, (iv) 2.0 mg/I BAP + l.0 mg/I IAA, (v) 0.5 mg/I BAP + 1.0 mg/I NAA, (vi) 2.0 mg/I BAP + 0.1 mg/I IAA, (vi i) 3.0 mg/I BAP + 1.0 mg/I NAA + 1.5 mg/I Kn. Activated charcoal was used in some of these media combinations as an adsorbent. pH of the media was adjusted to 5.8 in MS and 5.4 in MVW, respectively. Subculturing was done regularly at an interval of 25 - 30 days.

Source of Information: J. Asiat. Soc. Bangladesh, Sci. 31(1): 15-20, June 2005

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Inducing flowering under controlled conditions is quite a tedious and frustrating experience. The extent of manipulations for example, media, additives and environment (photoperiod, temperature) could be numerous. It is difficult to generalize the requirements to achieve in vitro flowering. Although in the recent years, many attempts on in vitro flowering have been successful, the direct role of involved factors till now is obscure, specially about the hypothetical 'florigenic hormone'. On the whole, this success of flower induction in D. lindleyi and D. tortile has a certain appeal in physiological study and commercial market.

Knowledge Management: Journal