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In Vitro Propagation of Muskmelon (cucumis Melo L.) from Nodal Segments, Shoot Tips and Cotyledonary Nodes

S. Parvin
Professor Joarder DNA & Chromosome Research Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi 6205, Bangladesh.

M. Kausar
Professor Joarder DNA & Chromosome Research Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi 6205, Bangladesh.

M. Enamul Haque
Professor Joarder DNA & Chromosome Research Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi 6205, Bangladesh.

M. Khalekuzzaman
Professor Joarder DNA & Chromosome Research Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi 6205, Bangladesh.

B. Sikdar
Professor Joarder DNA & Chromosome Research Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi 6205, Bangladesh.

M. Asadul Islam
Professor Joarder DNA & Chromosome Research Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi 6205, Bangladesh.Corresponding author: asadgen@ru.ac.bd

Abstract/ Executive Summary:

A rapid and efficient protocol is outlined for in vitro propagation of muskmelon (Cucumis melo L.) Shoot tips, nodal segments and cotyledonary nodes from in vitro grown seedlings were used as explants. The explants were inoculated on MS medium fortified with different combinations and concentrations of growth regulators viz., BAP, NAA, GA₃ and IBA for multiple shoot regeneration. Effective result was found on MS medium supplemented with 2.0 mg/l BAP, in which 90% and 70% cultures induced multiple shoots from nodal segments and shoot tip explants, respectively. Whereas, 70% cultures of cotyledonary nodes were found to induced shoots on MS medium with 1.5 mg/l BAP + 0.1 mg/l GA₃. In vitro regenerated shoots were subcultured on half strength MS medium supplemented with different concentrations of IBA and NAA for successful root induction and the effective result (up to 70%) was found in medium with 1 mg/l IBA. Well rooted in vitro grown plantlets were acclimatized in sandy soil, where as 70% plantlets survived.

Keywords: In vitro propagation, Nodal segments, Shoots tip, Cotyledonary nodes and Cucumis melo.

Location: Prof. Joarder DNA and Chromosome Research Lab., Department of Genetic Engineering and Biotechnology, University of Rajshahi

Start Date:

End Date:

Program Area: Crop-Soil-Water Management

Commodity: Water melon

Objectives:

In the present investigation, attempt has been taken to develop a suitable in vitro direct regeneration protocol from nodal segments, shoot tips and cotyledonary nodes of muskmelon.

Methodology:

The study was carried out at Prof. Joarder DNA and Chromosome Research Lab., Department of Genetic Engineering and Biotechnology, University of Rajshahi. Different explants viz., nodal segments, shoot tips and cotyledonary nodes were collected from in vitro grown seedlings of muskmelon (local cultivar) and seeds were collected from local market. The explants were collected carefully as per explants requirement and subsequently transferred to laminar airflow cabinet. These explants were cultured in MS (Murashige & Skoog, 1962) medium supplemented with 30g/l sucrose, 7g/l solidifying agar. The pH of the medium was adjusted, in every case, to 5.6-5.8 and 10 ml of medium was taken in each test tube which was covered by non-absorbent cotton plug. After preparation, medium was autoclaved for 21 minutes at the pressure of 1.5 kg/cm² at 121°C. Plant growth regulators were used in different concentrations and combinations as for direct shoot and root induction. BAP (0.5-3 mg/l), NAA (0.1- 0.5 mg/l) and GA3 (0.1- 0.3 mg/l) were used for multiple shoot regeneration. NAA (0.1- 0.5 mg/l) and IBA (0.5- 3.0 mg/l) were used for root induction. The inoculated cultures were kept in dark condition for 24 hrs and then they were transferred to growth chamber, where the fluorescent light intensity was 2500-3000 lux and the room temperature was 25±2°C. Subculture was done 21 days intervals and every experiment was repeated twice. Data were recorded after 15 and 21 days of culture. After complete rooting micro plants were acclimatized in sandy soil and established finally in humus soil with careful maintenance.

Source of Information: Rajshahi University journal of life & earth and agricultural sciences, Vol. 41: 71-77, 2013 ISSN 2309-0960

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

In vitro root induction was conducted on half strength MS with different concentrations of IBA and NAA. Better root induction was observed in IBA. Plantlets were successfully established in soil with 70% survival rate. A suitable protocol has been established for in vitro direct regeneration. An effective growth

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