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Evaluation of Two Plant Materials for the Management of Pulse Beetle, Callosobruchus Chinensis L.

S.M.H Begum
Department of Entomology, Bangladesh Agricultural University, Mymensingh-2202

M.J. Alam
Entomology Division, Bangladesh Institute of Nuclear Agriculture, Mymensingh

Masum Ahmad
Department of Entomology, Bangladesh Agricultural University, Mymensingh-2202

K.R. Das
Department of Entomology, Bangladesh Agricultural University, Mymensingh-2202

Abstract/ Executive Summary:

An investigation was carried out with acetone and water extracts of leaf and seed of castor, Ricinus communis and urmoi, Sapium indicum for their direct toxic and residual effects on the pulse beetle, Callosobruchus chinensis using different (7.5, 10, 12.5 and 15%) concentrations. Both urmoi and castor seed extract was found toxic for pulse beetle having 34.44% and 34.33% mortality respectively. The seed extract was more effective than that of leaf extract. Again the acetone extracts of leaf and seed were more effective than those of water extracts.

Keywords: Plant extract, Toxicity, Residual effect, Pulse beetle

Location: Department of Entomology

Start Date: 00-05-2006

End Date: 00-10-2006

Program Area: Pest Management

Commodity: Insects

Objectives:

1. An investigation was carried out with acetone and water extracts of leaf and seed of castor, Ricinus communis and urmoi, Sapium indicum for their direct toxic and residual effects on the pulse beetle, Callosobruchus chinensis using different (7.5, 10, 12.5 and 15%) concentrations.

Methodology:

The test insect, C. chinensis were collected from the pulse godown of Mymensingh town and were reared in the laboratory to get sufficient number of adult beetles for conducting the whole experiment. Fresh leaves and seeds of urmoi were collected from rural areas of Phatuakhali, and from Boira and Sutiakhali villages of Mymensingh district. After collection, fresh leaves and seeds were washed in running tap-water and air dried in the shade for 15 days and then oven dried at 60⁰C to make dust. Oven dried leaves and seeds were ground to powder in an electric grinder. A 25 mesh diameter sieve was used to obtain fine dust. Twenty gm of each category of dust were taken in a 600 ml beaker and separately mixed with 200 ml of selected solvents (distilled water and acetone). Then the mixture was stirred for 30 minutes by a magnetic stirrer (at 6000 rpm) and left to stand for next 24 hours. The mixture was then filtered through a filter paper (Whatman No.1). Different concentrations of each plant extracts (7.5%, 10%, 12.5% and 15%) were prepared with respective solvents. The adult insects were chilled for a period of 10 minutes. Then the immobilized insects were picked up individually by using a camel hair brush. Two μl solution of different concentrations were applied to the dorsal surface of the thorax of each insect using a fine micropipette. Ten insects per replication were treated and each treatment was replicated 3 times. In addition, the same number of insects were treated with respective solvent only for control. After treatment, the insects were transferred into 9 cm diameter petridishes (10 insects/petridish) containing food. Insect mortalities were recorded at 24, 48 and 72 hours after treatment (HAT). Observed mortalities of the insects were corrected. Corrected mortality =[(observed mortality–control mortality)/control mortality] × 100, then it was analyzed by ANOVA. The mean values were separated by DMRT. The corrected mortality data were analyzed by probit analysis.

Source of Information: J. Agrofor. Environ. 1(2): 71-75, 2007; ISSN 1995-6983

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Both urmoi and castor seed extract was found toxic for pulse beetle having 34.44% and 34.33% mortality respectively. The seed extract was more effective than that of leaf extract. Again the acetone extracts of leaf and seed were more effective than those of water extracts.

Knowledge Management: Journal