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In Vitro Regeneration Performance of Corchorus Olitorius

M. Hoque
Dept. of Agronomy and Seed Science, Sylhet Agricultural University, Sylhet; Bangladesh

K. M Nasiruddin
Dept. of Biotechnology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

G. K. M. N. Haque
Department of Agriculture Extension, Dhaka, Bangladesh.

G. C. Biswas
Dept. of Biotechnology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Abstract/ Executive Summary:

The experiment was conducted during May to December 2008 in the Biotechnology Laboratory of Bangladesh Agricultural University, Mymensingh to observe the callus induction, regeneration potentiality and to establish a suitable in vitro plantlet regeneration protocol of Corchorus olitorius. MS medium supplemented with different phytohormone concentrations and combinations were used to observe the callus induction, shoot regeneration and root formation ability of the cotyledon with attached petiole derived explant of three genotypes viz. O-9897, O-72 and OM-1. The highest callus induction (92.85%) was observed in O-9897 followed by O-72 (82.14%) in the MS media supplemented with 2.5 mg/L BAP + 0.5 mg/L IAA. Genotype O-9897 in MS media supplemented with 2.5 mg/L BAP + 0.5 mg/L IAA produced the highest percentage of shoot regenerants (83.33%) followed by O-72 (75.00%) in the media supplemented with 2.5 mg/L BAP + 0.5 mg/L IAA. The root formation from regenerants was the best on half strength of MS media supplemented with 0.6 mg/L IBA in genotype O-9897 (45.00%). The in vitro regenerated plantlets from the genotypes O-9897 could be established in the field. Therefore, the genotypes O-9897 of C. olitorius in MS media supplemented with 2.5 mg/L BAP + 0.5 mg/L IAA could be used for callus induction and shoot regeneration.

Keywords: Regeneration, Phytohormone, Corchorus olitorius

Location: Biotechnology Laboratory of Department of Biotechnology, Bangladesh Agricultural University, Mymensingh

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Jute

Objectives:

To optimize the hormonal concentration for in vitro regeneration performance of different genotypes of C. olitorius

Methodology:

The experiment was conducted during May to December, 2008 in the Biotechnology Laboratory of Department of Biotechnology, Bangladesh Agricultural University, Mymensingh to observe the regeneration potentiality of three genotypes of C.olitorius viz.O-9897, O-72 and OM-1. Culture Media: Half strength MS (Murashige and Skoog, 1962) medium supplemented with clinical cotton or agar was used for seed germination. For callus induction and shoot regeneration, MS media supplemented with a single concentration of IAA (0.5 mg/L) and four concentrations of BAP (1.5, 2.5, 3.5 and 4.5 mg/L) were used in four combinations. Half strength MS media supplemented with four concentrations of IBA (0.2, 0.4, 0.6 and 0.8 mg/L) were used for root initiation. Soil containing 25% garden soil, 50% sand and 25% cowdung was used for transplanting of plantlets from culture vessel to pot. Culture Techniques:Twenty five sterilized seeds were placed into sterilized seed germination medium in each vial. The culture was then incubated in dark till germination of the seeds and then transferred to 16 hours light for normal seedling growth. Cotyledons with attached petiole of seven days old seedlings were used as explant. Seven explants from each genotype were incubated in culture vial for callus formation. Six calli of 20-25 mm in diameter from each genotype were again cultured in MS media containing different concentrations and combination, of IAA and BAP for shoot regeneration. After shoot regeneration, five calli of each genotype were cultured in vials with freshly prepared root induction medium to form root. The culture vials were incubated at 22 ± 2°C with 16 hours photoperiod for callus formation, shoot regeneration and root development. When the plantlets become 5-7 cm in length having enough root system, they were taken out from the vials and then transplanted to pots containing of garden soil, sand and cowdung. To resist sudden stress, the pots were kept in a growth room for 10-15 days under controlled environment covered with moist polythene. After two to three days the polythene bags were partially removed and completely removed when the complete plantlets were seem to be self-sustainable. Recording DataRecording Data: Data on callus initiation,shoot regeneration and root formation were recorded.

Source of Information: J. Bangladesh Agril. Univ. 8(1): 1–6, 2010 ISSN 1810-3030

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The highest callus induction (92.85%) was observed in O-9897 followed by O-72 (82.14%) in the MS media supplemented with 2.5 mg/L BAP + 0.5 mg/L IAA. Genotype O-9897 in MS media supplemented with 2.5 mg/L BAP + 0.5 mg/L IAA produced the highest percentage of shoot regenerants (83.33%) followed by O-72 (75.00%) in the media supplemented with 2.5 mg/L BAP + 0.5 mg/L IAA. The root formation from regenerants was the best on halfstrength of MS media supplemented with 0.6 mg/L IBA in genotype O-9897 (45.00%). The in vitro regenerated plantlets from the genotypes O-9897 could be established in the field. Therefore, the genotypes O-9897 of C. olitorius in MS media supplemented with 2.5 mg/L BAP + 0.5 mg/L IAA could be used for callus induction and shoot regeneration

Knowledge Management: Journal