Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Artificial Seed Production in Pointed Gourd (trichosanthes Dioica Roxb.)

M. A. Malek
Plant Genetic Resources Centre, Bangladesh Agricultural Research Institute, Gazipur 1701, Bangladesh

M. O. Islam
Plant Genetic Resources Centre, Bangladesh Agricultural Research Institute, Gazipur 1701, Bangladesh

M. A. Bari Miah
Professor
Institute of Biological Sciences (IBSc.), University of Rajshahi, Rajshahi-6205

Abstract/ Executive Summary:

Encapsulation of nodal segments was successfully developed for pointed gourd towards the formation of artificial seed with sodium alginate. Encapsulated nodal segments (artificial seed) were cultured in MS basal medium containing different concentrations and combinations of BAP and NAA to induce germination and shoot proliferation. Highest (95%) shoot formation was obtained in MS + 1.0 mg/l BAP followed by MS + 0.5 mg/l BAP. The encapsulated nodal segments also regenerated in vitro on different substrates. Frequency of plantlet formation was low on these substrates compared to plantlet development on MS media. Among these substrates, the percentage of plantlet formation was better on moist cotton (42%) followed by filter paper (35%). The hardened plants were transferred successfully to soil in the earthen pots. The protocol for encapsulating the nodal segments for the production of artificial seeds and their subsequent regeneration is a new area of research to develop in vitro conservation strategies for pointed gourd.

Keywords: Pointed gourd, Plant propagation, Artificial seed

Location: Tissue Culture Laboratory, Biotechnology Division, BARI, Joydebpur, Gazipur 1701

Start Date:

End Date:

Program Area: Seed Technology

Commodity: Pointed gourd

Objectives:

The present study describes the production of artificial seeds from encapsulation of nodal segments of pointed gourd through solid cultures. This method provides a mechanism for handling tissue cultured plants in a manner similar to that of plants grown from seeds.

Methodology:

The experiments were conducted in the Tissue Culture Laboratory, Biotechnology Division, BARI, Joydebpur, Gazipur 1701. Nodal segments (3-4 mm) were aseptically excised from in vitro grown plantlets of two pointed gourd cultivars (AM-8 and AM-15) regenerated by the method described by Maruyama (1996). Data on days to shoot proliferation and percentage of shoot forming explants were recorded after culture of encapsulated nodal segments. Culture media preparation Culture media were prepared following MS (Murashige and Skoog, 1962) media and 8.0 gram agar was added to each 1000 ml solution. Preparation of Alginate and CaCl₂ solution Media preparation: To prepare 200 ml MS medium 4% sucrose was first added to 150 ml of solution and then different concentrations and combinations of BAP (0.5, 1.0, 1.5 and 2.0 mg/l) and NAA (0.5, 1.0, 1.5 and 2.0 mg/l) were added to it. After mixing the solution was filled up to 200 ml. Alginate solution 20 ml of the above mentioned solution (MS + Sucrose + hormones) was taken in a 50 ml beaker and 0.8 gm of sodium alginate was added and mixed with a glass rod. The alginate was completely dissolved during autoclaving CaCl₂ solution 50 ml of above mentioned solution (MS + Sucrose + hormones) was taken in a small beaker. An amount of 0.7 gm CaCl₂ was added to it and dissolved. Out of 200 ml, 70 ml (50+20) was used during the preparations of alginate and CaCl₂ solution and remaining 130 ml was used for washing the encapsulated explants. Culture media, alginate solution, CaCl₂ solution and the washing solution were autoclaved for 20 minutes under 121⁰C. Encapsulation Sodium alginate beads were produced by encapsulation according to the method of Kinoshita and Saito (1990). Only in vitro grown plantlets were used for this experiment. In vitro plantlets were taken in autoclaved petridishes and nodal segments were cut carefully. The excised nodal segments with active buds were placed in a beaker containing sodium alginate solution. The explants were kept in the solution for about 30 minutes. Then they were placed in the beaker of CaCl₂ solution with a pair of forceps. During picking the forceps also took some alginate solution together with the explants. After 30 minutes each explant become a hard ball encapsulated by alginate. They were then washed well with remaining solution of MS + Sucrose + growth regulators. After washing, the encapsulated nodal segments were cultured in culture tubes containing MS medium supplemented with different concentrations and combinations of BAP (0.5, 1.0, 1.5 and 2.0 mg/l) and NAA (0.5, 1.0, 1.5 and 2.0 mg/l) and also on various substrates such as sterile soil, cotton and filter paper moistened with half strength MS solution. The germination responses (viz. development of plants) of the encapsulated nodal buds were scored after three weeks of culture. For each treatment 20 nodal buds were cultured and all the experiments were conducted under controlled conditions of light (1000 lux), temperature (25 + 2⁰C) and relative humidity (50-60%).

Source of Information: Bangladesh J. Genet Pl. Breed., 21(1): 15-20, 2008

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

In the present investigation, nodal segment was successfully encapsulated by sodium alginate to perform as artificial seed beads. Artificial seeds developed from nodal segments were also subjected to hormonal treatments in order to enhance germination. It is the first report in Bangladesh to develop artificial seed production using vegetative tissue of a crop plants.

Knowledge Management: Journal