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Inheritance of Fertility Restoration Involving Wild Abortive Cytoplasmic Male Sterility System in Rice (oryza Sativa L.)

M. J. Hasan
Hybrid Rice Section Bangladesh Rice Research Institute, Gazipur 1701, Bangladesh.

M. U. Kulsum
Hybrid Rice Section Bangladesh Rice Research Institute, Gazipur 1701, Bangladesh.

A. Ansari
Hybrid Rice Section Bangladesh Rice Research Institute, Gazipur 1701, Bangladesh.

A. K. Paul
Hybrid Rice Section Bangladesh Rice Research Institute, Gazipur 1701, Bangladesh.

P. L. Biswas
Hybrid Rice Section Bangladesh Rice Research Institute, Gazipur 1701, Bangladesh.

Abstract/ Executive Summary:

Inheritance of fertility restoration was studied in crosses involving ten elite restorer lines of rice viz. BR6839-41-5-1R, BR7013-62-1-1R, BR7011-37-1-2R, BR10R, BR11R, BR12R, BR13R, BR14R, BR15R and BR16R and one male sterile line Jin23A with WA sources of cytoplasmic male sterility. The segregation pattern for pollen fertility of F₂ and BC₁ populations of crosses involving Jin23A indicated the presence of two independent dominant fertility restoring genes. The mode of action of the two genes varied in different crosses revealing three types of interaction, i.e. epistasis with dominant gene action, epistasis with recessive gene action, and epistasis with incomplete dominance.

Keywords: Rice, CMS, Fertility restoration, Inheritance

Location: BRRI.Gazipur

Start Date: 00-00-2008

End Date: 00-00-2010

Program Area: Variety and Species

Commodity: Rice

Objectives:

To determine the genetic control of fertility restoration of WA-CMS system.

Methodology:

The experiment was conducted at the experimental field of Bangladesh Rice Research Institute (BRRI), Gazipur. Three consecutive seasons such as Boro 2008-09, T. Aman 2009 and Boro 2009-10 has been deployed for this experiment. The material consisted of one male sterile line Jin23A belonging to WA type source of cytoplasmic male sterility and ten prospective restorer lines. The restorer lines were BR6839-41-5- 1R, BR7013-62-1-1R, BR7011-37-1-2R, BR10R, BR11R, BR12R, BR13R, BR14R, BR15R and BR16R. First season F₁ was raised. The resulting F₁’s were selfed as well as backcrossed with their respective female parents to generate F₂ and BC₁ populations in the second season and final season evaluation was made with pollen and spikelet fertility of each plant in the F₂ and BC₁ populations. From each plant, five anthers from different spikelets were collected and their pollen grains were stained in 1% Iodine Potassium Iodide (IKI) solution. Plants were classified on the basis of pollen fertility analysis as fertile (61-100% pollen stained), partially fertile (31-60%), partially sterile (1-30%) and sterile (<1%) based on their shape and extend of staining under an optical microscope. One panicle from each plant was bagged before flowering for spikelet fertility analysis and spikelet fertility of bagged panicle was count at maturity stage. At maturity, the bagged panicle was examined for seed set and classified as fertile (81-100% seed set), partially fertile (31-80%), partially sterile (1-30%) and sterile (<1%). The parental lines, F₁ progenies, 250 F₂ plants for each segregating population were grown and evaluated in the same conditions for phenotypic and pollen fertility rate was used as the main criteria for the evaluation of fertile and sterile plants. F₁’s contained two rows, F₂ seven rows, BC₁ five rows with 37 plants/row and non replicated. For inheritance analysis plants with less than 1% stained pollen were categorized into the sterile class, and all others were regarded as fertile. Chi-square analysis was used to estimate the distribution pattern of Rf alleles with WA type source of CMS lines. Measurement of pollen fertility was made on all plants in each of the entries. Twenty to thirty spikelets were collected from each primary panicle and fixed in 70% alcohol. From these spikelets 10-12 anthers were collected at random and smear in IKI (1%) and examined under optical microscope. Plants were classified on the basis of pollen fertility analysis as fertile (61-100% pollen stained), partially fertile (31-60%), partially sterile (1-30%) and sterile (<1%) based on their shape and extend of staining under an optical microscope.. The pollen fertility were computed and expressed in percentage for each F₁, F₂ and BC₁ population as follows:-Pollen fertility (%)=(Number of fertile pollen grains/Number of steril pollen grains+Number of fertilepollen grainx100) .

Source of Information: Bangladesh J. Pl. Breed. Genet, 24(1): 33-40

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The segregation pattern for pollen fertility of F₂ and BC₁ populations of crosses involving Jin23A indicated the presence of two independent dominant fertility restoring genes. The mode of action of the two genes varied in different crosses revealing three types of interaction, i.e. epistasis with dominant gene action, epistasis with recessive gene action, and epistasis with incomplete dominance.

Knowledge Management: Journal