Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Plant Regeneration from Seedling Derived Explants Through Callus of Eggplant (solanum Melongena L)

B. P. Ray
Biotechnology Laboratory, Department of Biotechnology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

L. Hassan
Biotechnology Laboratory, Department of Biotechnology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

S. K. Sarker
Biotechnology Laboratory, Department of Biotechnology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Abstract/ Executive Summary:

Different concentrations and combinations of hormones were used in MS medium to observe callus induction and then plant regeneration using stem, leaf and root explants. The rate of callus formation varied in different treatments used. The highest amount of callus (48.66%) was produced on MS medium containing 2.0 mg/l BAP and 0.5 mg/l NAA from stem after 8.2 days. The highest fresh weight of callus was 1.12, 0.5 and 0.48 g from stem, leaf and root, respectively. The highest percentage of regeneration (23.28%) was recorded in MS media containing 2.0 mg/l BAP + 0.5 mg/l NAA from stem after 38.8 days. However, 11.94% leaf the regeneration was obtained by 2.0 mg/l BAP + 0.5 mg/l NAA after 46.2 days. It was also found that 2.0 mg/l BAP + 0.5 mg/l NAA was the best stem regeneration. The protocol in the study might be useful for the production of disease free and healthy plant materials and also it would be useful for genetic transformation of eggplant using biotechnological approach.

Keywords: 6-Benzyl amino purine (BAP), Naphthalene acetic acid (NAA), Solanum melongena, Regeneration.

Location: Biotechnology Laboratory, Department of Biotechnology, Bangladesh Agricultural University Mymensingh-2202, Bangladesh

Start Date:

End Date:

Program Area: Variety and Species

Commodity: Brinjal

Objectives:

To select somaclonal variants of eggplant genotypes tolerance to brinjal shoot and fruit borer insect based on biochemical markers.

Methodology:

Healthy seeds of eggplant cv. Jhumki were collected from Bangladesh Agricultural Research Institute (BARI). The seeds were then washed thoroughly in running tap water. The surface sterilization of these seeds was carried out under a Laminar Air Flow Cabinet. The floated seeds were discarded and others were rinsed in 70% ethyl alcohol for one minute, and then thoroughly washed with sterilized distilled water. The alcohol treated seeds were immersed into 0.1% HgCl₂ solution for 8-10 minutes, few drops Tween-20 per 100 ml was also added at that time. Sterilized six seeds were placed into seed germination medium in six Petri dishes and then incubated in dark till the germination. The experiment was conducted in a randomized complete block design with 16 treatments replicated. Six pieces of each segment were arranged horizontally on each petridish and gently pressed into the surface of the sterilized culture medium with various concentrations and combinations of hormones. The number of callus initiated over a number of days was recorded. Percent callus induction was calculated on the basis of the number of explant placed and the number of callus induced. Weight of callus, Days of shoot initiation, Number of callus with shoot, Number of shoot per callus, Number of shoot with root, Number of regenerated plantlets were recorded. The plantlets with well-developed root system were removed from culture vessels with care and without damaging the roots. The agar was removed from the roots by washing with running tap water. After washing, the plantlets were transferred to small earthen pots filled with 1:2:1 of sand, soil and cow dung mixture. The plantlets were kept in diffused sunlight covered with polythene bag to prevent desiccation. Adequate moisture was supplied for 10 days and gradual exposure to air and light was allowed. After 30 days, the plantlets became 30 cm long and the survival rate was 75%. The analysis of variance for different characters was performed and means were compared by the Duncan’s Multiple Range Test (DMRT).

Source of Information: The Agriculturists 8(2): 98-107 (2010) ISSN-1729-5211, A Scientific Journal of Krishi Foundation

Article Link (Online Journal): http://www.banglajol.info/index.php/AGRIC/article/view/7583/5722

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Explants were cultured on MS media supplemented with different combinations and concentrations of BAP (0, 2.0, 3.0 and 4.0 mg/l) and NAA (0, 0.1, 0.5, and 1.0 mg/l). The highest amount of callus (48.66%) was produced on MS medium containing 2.0 mg/l BAP and 0.5 mg/l NAA from stem after 8.2 days. The growth of callus was faster on MS media supplemented with 2.0 mg/l BAP and 0.5 mg/l NAA from the stem. The highest fresh weight of callus was 1.120 g from the stem explants and 0.5g from the leaf. The maximum number of plant regeneration through callus from stem containing 2.0 mg/l BAP and 0.5 mg/l NAA were 3.4 (23.287%) and from leaf containing 2.0 mg/l BAP and 0.5 mg/l NAA were 1.6 (11.94%). The best number of shoot regenerated through callus from stem containing 2.0 mg/l BAP and 0.5 mg/l NAA was 3.4 (23.287%) and days required for 38.8 days.

Knowledge Management: Journal