Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Tissue Distribution of B-lymphocyte Subsets (iga, Igg and Igm) in the Mucosa and Lymphoid Tissues of Broilers Immunized with Gumboro Vaccine

M. T. Hussan
Department of Anatomy and Histology, Faculty of Animal Science and Veterinary Medicine, Patuakhali Science and Technology University, Babugonj, Barisal-8210, Bangladesh.

M. Z. I. Khan
Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University,Mymensingh-2202, Bangladesh.

M. R. Jahan1
Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University,Mymensingh-2202, Bangladesh.

Abstract/ Executive Summary:

The present research was designed to study the histological and immunohistochemical changes of lymphoid (bursa of Fabricius and cecal tonsil) and mucosal (ileum) tissues of broiler chickens after immunization with Gumboro vaccine. Two groups (n=24) of Cobb-500 broiler chickens were reared in the same environment. A mild vaccine (BUR® 706) followed by an intermediate plus (Nobilis®Gumboro 228 E) vaccine was administered for immunization of chickens and samples (bursa of Fabricius, cecal tonsil and ileum) were collected at 7 days interval for up to 32 days of age. In the bursa of Fabricius, the population of lymphocytes and the size of the follicles were found to increase in vaccinated chickens than the control chickens. The Igs positive cells (IgA, IgG and IgM) were distributed principally beneath the capsule, around the follicles and in the cortex and medulla of bursa of Fabricius. The frequencies of IgG- and IgM- positive cells were intense than the IgA positive cells in the bursa of Fabricius vaccinated chickens. IgM-positive cells became peak at day 25 and started to decline at day 32 in the bursa of Fabricius of vaccinated groups of chickens. In the cecal tonsils and ileum, the Igs positive cells (IgA, IgG and IgM) were distributed around the intestinal gland and within the lymphatic nodules of the lamina propria, in the core of the villi, and within the epithelium in both the control and vaccinated chickens. Their frequency per 0.1 mm2 area of the lamina propria and in the epithelium was increased abruptly in the vaccinated group than the control broiler chickens. Like bursa, in the cecal tonsil and ileum of vaccinated chickens, the frequencies of IgG- and IgM-positive cells were also abundant than IgA-positive cells. The present study suggested that the Igs positive cells were greatly accelerated in the vaccinated chickens than the control chickens and it may be due to immunomodulatory effect of vaccine.

Keywords: B lymphocyte, Broilers, Lymphoid tissues, Gumboro vaccine

Location:

Start Date:

End Date:

Program Area: Animal Health and Management

Commodity: Poultry

Objectives:

1.To study the histology of the lymphoid (bursa of Fabricius and cecal tonsils) and mucosal organ (ileum) of broiler chickens

2.To study the frequency of the population of lymphocytes and plasma cells containing different classes of immunoglobulins (IgA, IgG and IgM) in those tissues following vaccination of chickens with infectious bursal disease virus vaccines.

Methodology:

Chickens A total 24 (twenty four) day-old “Cobb-500” broiler chickens of both sexes were purchased from “Kazi Farm Ltd.” First sample was collected from 3 chickens at day 3 (pre-vaccination), second sample was collected from chickens at day 11 (pre-vaccination) and remaining chickens (n=18) were divided equally into vaccinated group and control group. First vaccine was administered at the same day (day 11) to 9 broiler chickens using BUR®- 706 vaccine (Merial, France) through intraocular route, 1 drop in each eye. At day 18, sample (n=3) was collected from both group (3rd sample) and second dose of vaccine was administered to remaining 6 chickens of vaccinated group using Nobilis® Gumboro 228E (Intervet, The Netherlands) through intraocular route at day 18. Later on fourth and fifth samples were collected at day 25 (n=3) and day 32 (n=3), respectively from both vaccinated and control group. For histological and immunohistochemical staining purpose, the bursa of Fabricius, cecal tonsils and ileum was collected, after sacrificing the chickens through cervical subluxation method. Preparation of samples for histological studies For histological studies all the samples were cut into pieces and then fixed in the “Bouin’s fluid” (Gridley, 1960), dehydrated in a series of ascending grades of alcohol, cleared in several changes of xylene, and infiltrated with different grades of melted paraffin in the oven. The tissues were then embedded in paraffin and finally the sections were cut at 6-μm thickness using sliding microtome (MIC 509, Euromex, Japan). The sections were then stained with Hematoxylin and Eosin staining method (Gridley, 1960). Antibodies The antibodies for detecting Igs-containing plasma cells used in this experiment were normal rabbit serum (Biosource, Camarillo, California, USA), goat anti-chicken IgA (Bethyl Lab, USA), goat anti-chicken IgG (Bethyl Lab, USA), goat anti-chicken IgM (Bethyl Lab, USA), and HRP-conjugated rabbit anti-goat IgG (Bethyl Lab, USA). Immunohistochemical staining and histoplanimetry Indirect immunoperoxidasestainingmethod was done for the study of the distributional pattern and frequency of the Ig-containing plasma cells in the lymphoid (bursa of Fabricius and cecal tonsils) and mucosal (ileum) organ of vaccinated and control broilers. The tissues were fixed in ice-cold PLP (Periodate-lysineparaformaldehyde), dehydrated in a series of graded alcohol, cleared in xylene, and embedded in paraffin. Paraffin sections, 6 μm thickness, were immunostained by the indirect immunoperoxidase method as described earlier (Khan et al., 1997 and 2007). The immunopositive cells (Igs-positive cells) in the lymphoid tissues and mucosa were counted in 20 fields at a magnification of 40 according to Weibel (1969) and their relative frequency per 0.1 mm2 was calculated using ocular micrometer. Statistical analysis All values were expressed as Mean±SE. Statistical analyses were performed by independent sample t test. Significance was established when the probability level was equal to or less than 5%.

Source of Information: Bangl. J. Vet. Med. (2013). 11 (1):13-19 ISSN: 1729-7893 (Print) 2308-0922 (Online)

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

In the bursa of Fabricius, the population of lymphocytes and the size of the follicles were found to increase in vaccinated chickens than the control chickens. The Igs positive cells (IgA, IgG and IgM) were distributed principally beneath the capsule, around the follicles and in the cortex and medulla of bursa of Fabricius. The frequencies of IgG- and IgM- positive cells were intense than the IgA positive cells in the bursa of Fabricius vaccinated chickens. IgM-positive cells became peak at day 25 and started to decline at day 32 in the bursa of Fabricius of vaccinated groups of chickens. In the cecal tonsils and ileum, the Igs positive cells (IgA, IgG and IgM) were distributed around the intestinal gland and within the lymphatic nodules of the lamina propria, in the core of the villi, and within the epithelium in both the control and vaccinated chickens. Their frequency per 0.1 mm2 area of the lamina propria and in the epithelium was increased abruptly in the vaccinated group than the control broiler chickens. Like bursa, in the cecal tonsil and ileum of vaccinated chickens, the frequencies of IgG- and IgM-positive cells were also abundant than IgA-positive cells. The present study suggested that the Igs positive cells were greatly accelerated in the vaccinated chickens than the control chickens and it may be due to immunomodulatory effect of vaccine.

Knowledge Management: Journal