Agricultural Research Management Information System

Home
Research Summary
Search
About Us
- ARMIS
- Brochure
Contact Us
Report
User Request
Data Input
Help
- Operation Manual
  - PDF
  - Video
- Program Area & Commodity
We have reached 37600 number of research entries at this moment.

- Logout

Research Detail

Home
Research
Detail

Determination of Immune Response of Imported Newcastle Disease Virus Vaccines in Broiler Chickens

M. S. Islam
Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh

A. K. M. Khasruzzaman
CP Bangladesh Co. Ltd.

M. T. Hossain
Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh

M. T. Islam
Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh

M. H. Chowdhury
Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh

M. M. Rahman
Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh

M. A. Zinnah
Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh

N. A. Banu
Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh

M. A. Islam
Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh

Abstract/ Executive Summary:

A study was undertaken to determine the immune response of eight different imported live NDV vaccines in broiler chickens in the Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh during the period from July to December 2008. A total of 55 broiler chickens (Ross breed) were divided into eleven groups such as 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 and 11 of which group 1, 3, 5, 7 and 9 were vaccinated primarily with Nobilis® MA5+Clone30, Avipro ND-IB HB1, Cevac® BIL, Newcastle-Bronchitis Vaccine Fortdodge and Avipro ND LaSota vaccine respectively at day 5 of age and secondarily with Nobilis ND Clone 30, Avipro ND LaSota, Cevac New L, Newcastle Disease vaccine Fortdodge and Avipro ND LaSota vaccine respectively at day 21 of age by single eye instillation and 2, 4, 6, 8 and 10 were vaccinated with the same vaccines respectively by double eye instillation following the same schedule. Group 11 was kept as unvaccinated control. Sera samples were collected after 10 days of each vaccination and at day 5, 15, 20, 31 of age from nonvaccinated control and subjected to HI test for the determination of antibody titres. It was observed that after primary vaccination the geometric mean (GM) of HI titres of double eye vaccinated groups differed significantly (P<0.01). Overall analysis of GM of HI titres of double eye vaccinated groups revealed that there were significant increases (P<0.01) in HI titres in groups 8 (301.07±70.10) and 10 (284.24±85.86) compared to other groups. It was observed that secondary vaccination produced higher immune response compared to primary vaccination in case of all the vaccinated groups and double eye vaccination produced higher immune response compared to single eye vaccination in case of all the vaccinated groups. Maternal antibody was high (256.00±140.21) at day 5 of age and persisted to a minimal level (5.27±2.19) until the age of day 20 and almost disappears (≤4±0) at day 31 of age. From the present research it may be concluded that LaSota strain produced higher immune response than Clone 30 and B₁ strain and Fortdodge and Avipro vaccine produced higher immune response than all other vaccines used in this study.

Keywords: Immune response, Newcastle disease, ND vaccines, Broiler chickens

Location: Mymensingh

Start Date: 01-07-2008

End Date: 31-12-2008

Program Area: Pest Management

Commodity: Poultry

Objectives:

To determine the immune response of imported NDV vaccines (live) commonly used in broiler chickens in Bangladesh, to observe the persistence of maternally derived antibody against NDV in broiler chickens and also to compare the immune response of broiler chickens against each of the imported live NDV vaccines used in this study.

Methodology:

The whole experiment was conducted during the period from July to December 2008 in the Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh. A total number of 55 day-old-chicks of Ross breed broiler with the history of parent stock vaccinated against Newcastle disease (ND) were collected from the CP Bangladesh Co. Ltd., Dhaka and reared in the experimental shed of the Department of Microbiology and Hygiene, BAU, Mymensingh. The birds were reared in litter supplying feed (Nourish® poultry feed) and water maintaining strict bio-security. Before vaccination the birds were divided into 11 groups each group consists of 5 birds. Eight lyophilized (freeze-dried) Newcastle disease virus vaccines were procured from CP Bangladesh Ltd. and used in this study. These were (i) Nobilis® MA5+Clone30 vaccine (Clone30 and Massachusetts strain) of Intervet pharmaceuticals (ii) Avipro® ND-IB HB1 vaccine (B1 and Massachusetts strain) of Lohmann Animal Health (iii) Newcastle-Bronchitis Vaccine Fortdodge (B1 and Massachusetts strain) of Fortdodge Animal Health (iv) Cevac BIL vaccine (B1 and Massachusetts strain) of Ceva pharmaceuticals (v) Avipro ND LaSota (LaSota strain) of Lohmann Animal Health (vi) Nobilis ND Clone30 (Clone30 strain) of Intervet pharmaceuticals vii) Newcastle Disease Vaccine Fortdodge (LaSota strain) of Fortdodge Animal Health (viii) Cevac New L (LaSota strain) of Ceva pharmaceuticals. These vaccines were stored and diluted during use according to the instruction of the manufacturers

Source of Information: Bangl. J. Vet. Med. (2008). 6 (2): 139–144

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

it was observed that double eye vaccinated groups of broiler chickens produced significantly higher immune response (p<0.01) compared to those of single eye vaccinated groups of chickens. This increase immune response might be due to the higher number of virus present in each dose. The vaccine virus concentration was higher in double eye dose than the single eye dose which might be played a vital role in the production of higher immune response. For better immune response the dose of vaccine is important factor. which helps to increase the level of immunity also reported by Bankowski and Hill. Overall analysis of the results of the present study consideration among the vaccinated groups it was noticed that the HI titres of birds of group 8 vaccinated primarily with Newcastle- Bronchitis Vaccine Fortdodge and secondarily with Newcastle Disease Vaccine Fortdodge by double eye dose and group 10 vaccinated primarily and secondarily with Avipro ND LaSota by double eye dose were significantly (p<0.01) higher than all other vaccinated groups. Thus, considering the above findings it may be mentioned that Fortdodge vaccine and Avipro vaccine (Lohmann Animal Health) produced higher immune response than the other six vaccines used in this study.

Knowledge Management: Journal