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Culture of Brachionus Plicatilis Feeding with Powdered Dried Chlorella

S. Mostary
Department of Fisheries, Ministry of Fisheries and Livestock, Dhaka, Bangladesh

M. S. Rahman
Departments of Fisheries Management, Faculty of Fisheries, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

A. S. M. S. Mandal
Departments of Fisheries Management, Faculty of Fisheries, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

K. M. M. Hasan
Department of Zoology, Faculty of Biological Sciences, Rajshahi University, Rajshahi-6205, Bangladesh

Z. Rehena
Department of Zoology, Faculty of Biological Sciences, Rajshahi University, Rajshahi-6205,Bangladesh

S. M. A. Basar
Department of Fisheries, Ministry of Fisheries and Livestock, Dhaka, Bangladesh

Abstract/ Executive Summary:

The rotifer Brachionus plicatilis was cultured with powdered dried Chlorella in treatment 1, live or fresh cultured Chlorella in treatment 2, and baker’s yeast in treatment 3. All the jars under three treatments were stocked with B. plicatilis at the initial density of 10 individuals per ml. The water temperature, air temperature, pH and dissolved oxygen were within the suitable range for B. plicatilis culture. The highest population densities of B. plicatilis in treatments 1, 2 and 3 were 60000, 50000 and 30000 (individual/L), respectively. The powered dried Chlorella was comparable with live Chlorella and may be used successfully as a feed for B. plicatilis.

Keywords: Brachionus plicatilis, Feeding , Powdered dried Chlorella

Location: Faculty of Fisheries, Bangladesh Agricultural University, Mymensingh

Start Date:

End Date:

Program Area: Animal Health and Management

Commodity: Feed

Objectives:

The present study was undertaken to prepare an inexpensive culture medium using pulse bran to culture Chlorella for making powder to use as diet for rotifer and to compare the culture of rotifers with fresh Chlorella, powdered Chlorella and with baker’s yeast.

Methodology:

Maskalai (Vigna mungo) bran 700g was mixed with 20 litre tap water in three plastic 30 litre buckets. After one week 11g urea was added to each bucket. After four weeks partially decomposed bran mixture was filtered through thin clean cloth to remove solid particles. After a week the supernatant was siphoned to another bucket and 2g lime (Calcium oxide) per litre of medium was mixed to make it clear and pH was adjusted to seven using sulphuric acid. After a week the clear supernatant was again siphoned to another bucket as algae culture medium. The prepared medium was sterilized in an autoclave (121⁰C) under 15 1b/cm2 pressures for 15-20 minutes. Chlorella was cultured in this inexpensive organic medium for 18 days in 18 conical flasks (500 ml) arbitrarily marked 1-18, and each containing 200 ml of medium and Chlorella seed (Chlorella ellipsoidea, 20% or Chlorella ellipsoidea is 40 ml). Nine plastic jars were stocked with B. plicatilis at the initial density of 10 individuals/ml. Three treatments were used: in jars 1, 2, 3 (T-1) powdered dried Chlorella was used as feed for rotifer; in jars 4, 5, 6 (T-2) fresh cultured Chlorella was used; in jars 7, 8, 9 (T-3) baker’s yeast was used as feed. Pond water was collected in plastic bottles and centrifuged (2000 rpm) for 5 minutes to collect dense sample of phytoplankton. This was examined under a compound microscope to check for micro-alga. One or two drops of algae sample were taken using micropipette as inoculums in sterilized medium (5 or 10 ml) in test tubes. After about five days prospective cultures were examined and dominant culturable micro-algae were isolated using micropipette to culture in sterilized medium in glass tubes. After repeated cultures, seeds of Chlorella sp were collected C. ellipsoidea was cultured in the inexpensive organic medium. Twenty percent seeds of C. ellipsoidea were used as inoculum in 200 ml culture medium in 500 ml conical flask. The culture experiment was done in natural light and temperature on a glass shelf at 2nd floor facing the north where there was sufficient sunlight. Cell density of C. ellipsoidea collected from different conical flasks was estimated daily using haemocytometer as described by Rahman (1992). During the period of Chlorella culture the environmental factors were recorded daily. Cultured Chlorella was centrifuged (2000 rpm) for 5 minutes around 13th day of culture to collect the dense sample of Chlorella, which was preserved in the refrigerator. Supernatant was mixed with medium to use as Chlorella seed. The frozen Chlorella after thawing was dried in a microwave oven (SHARP, Japan), powdered with a mortar and pestle and kept in a glass tube with stopper for use as feed for rotifer. The seeds of rotifer were collected from different ponds of the Bangladesh Agricultural University campus through zooplankton nets. They were cultured in four plastic jars (5L) for two months, with continuous aeration by air pumps. Fresh cultured Chlorella was used as food. Samples were taken every day from each plastic jar for preservation (5% formalin) and daily analysed under a compound microscope using a special zooplankton counting cell until the concentration of rotifer was high without other contaminants. In T-1 (Jars 1, 2, 3) powdered dried Chlorella was given daily at the rate of 0.1g/L of water. In T-2 (Jars 4, 5, 6) fresh cultured Chlorella was given at the rate of 1.2 × 106 cells/ml and in T-3 (Jars 7, 8, 9) baker’s yeast was given daily at the rate of 0.2g/L. The Chlorella powder and the baker’s yeast were suspended in small amounts of water and homogenized. Continuous aeration was provided by aerators (SIGMA 4000 SW, Japan) connected by a narrow plastic pipe. Continuous lighting was provided. Physico-chemical parameters were recorded daily. B. plicatilis density was determined daily using a special zooplankton counting cell under a compound microscope. Analysis of variance (ANOVA) and Duncan’s Multiple Range Test (DMRT) of the experimental data were done by a computer.

Source of Information: The Bangladesh Veterinarian (2010) 27(2) : 91 – 98

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

It may be concluded that when live or fresh cultured Chlorella are not available then powdered dried Chlorella may be successfully used as a feed for B. plicatilis culture.

Knowledge Management: Journal