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Seroprevalence of Brucella Abortus Antibodies in the Cattle Population in the Selected Upazilas of Sirajgonj District

S. M. S. H. Belal
Surgeon, District Veterinary Hospital, Sirajgonj.

A. R. M. I. H. Ansari
Veterinary Surgeon, District Veterinary Hospital, Nilphamary

Abstract/ Executive Summary:

A study was carried out on 135 commercial dairy farms of five upazillas of Sirajgonj district to know the prevalence of Brucella abortus antibody in herds during the period from January 2012 to December 2013. A total of 270 blood samples were tested by Anigen® Rapid Brucella Ab test kit. The overall prevalence of bovine brucellosis was recorded as 8.51%. Distribution on the basis of breed, age, sex and pregnancy revealed the significant relationship among the infection, breed, sex, age and pregnancy in the population. The prevalence was relatively high in older cattle (9.09%) and the highest prevalence was 9.34% in the pregnant female. The prevalence of Brucella abortus antibody was considerably high in most of the large herds. Further study is needed to know the species and biovar of Brucella circulating in the study area.

Keywords: Brucellosis, Cattle, Seroprevalence, Anigen® Test Kit

Location: Sirajgonj

Start Date: 00-01-2012

End Date: 00-12-2013

Program Area: Animal Health and Management

Commodity: Cattle

Objectives:

To know the seroprevalence of brucellosis in cattle as measured by the Antigen rapid Brucella Ab test kit.

Methodology:

Location and duration of study: Five Upazillas of Sirajgonj district namely Sirajgonj Sadar, Shahjadpur, Ullapara, Kamarkhand and Belkuchi were selected on the basis of number and size the herds. Three administrative union from each Upazilla and three villages from each union were taken for this study. The study was conducted over a period of 24 months during January 2012 to December 2013.

Animal selection and sample collection: Three herds from each village and two cattle of over 6 months old from each herd were selected for the study. About 10 ml of whole blood sample was collected from the jugular vein, using plain vaccutainer tubes and needles from each cattle aged above six and with no history of vaccination for brucellosis. Each sample tube was labeled using codes specific to the individual sample. The tubes were tilted and serum was collected either passively by decanting or after centrifugation of the blood samples at 2,500 rpm for 5 min .The serum was stored at −20°C until tested serologically

Rapid kit test (Chromatographic Immunoassay): The samples were subjected to Anigen® Rapid B. Brucella Antibody Test Kit for the detection of Brucella abortus antibodies.

Source of Information: Bangl. J. Vet. Med. (2013).11(2): 127-130 ISSN: 1729-7893 (Print) 2308-0922 (Online)

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

Distribution on the basis of breed, age, sex and pregnancy revealed the significant relationship among the infection, breed, sex, age and pregnancy in the population. The prevalence was relatively high in older cattle (9.09%) and the highest prevalence was 9.34% in the pregnant female. The prevalence of Brucella abortus antibody was considerably high in most of the large herds. Further study is needed to know the species and biovar of Brucella circulating in the study area.

Knowledge Management: Journal