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Effects of Probiotics on Haematology and Biochemical Parameters in Mice

M Salahuddin
Department of Physiology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

H Akhter
Department of Physiology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

S Akter
Department of Physiology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

MA Miah*
Department of Physiology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

N Ahmad
Department of Physiology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

Abstract/ Executive Summary:

Probiotic organisms are live microorganisms thought to be beneficial to the host. To know the effect of probiotic supplemented food on haemato-biochemical parameters in mice, a total of 20 two-month-old Swiss Albino mice were randomly divided into four equal groups. Group A was control, fed commercial mouse pellets, and others were treated with 5 g (group B), 10 g (group C) and 20 g (group D) probiotics (curd), respectively, in mouse pellets for 45 days. Total erythrocyte count and haemoglobin concentration were significantly (P<0.01) higher in treated group than in controls. Total serum cholesterol, triglyceride, high density lipoprotein and low density lipoprotein values decreased significantly (P<0.01) in treated groups. Serum uric acid value was significantly higher (P<0.01) in treated groups. It is suggested that probiotics help improve haematology and lipid profile but not kidney function.

Keywords: Probiotics, Haematology, Biochemical parameters, Mice

Location: BAU, Mymensingh

Start Date:

End Date:

Program Area: Pest Management

Commodity: Rats

Objectives:

This study was undertaken to investigate the effect of dietary supplementation of probiotics (curd) on total erythrocyte count (TEC), haemoglobin (Hb) total cholesterol, triglyceride, high-density lipoprotein (HDL) lowdensity lipoprotein (LDL) and uric acid in the mice.

Methodology:

Animals: Twenty male Swiss Albino mice (Mus musculus) of six to eight weeks old, with an average body weight of 18 - 22g were obtained from International Centre for Diarrhoeal Diseases Research Bangladesh (ICDDR’B), Dhaka, Bangladesh. Standard mouse pellets were obtained from ICDDR’B. Before being used mice were allowed free access to a basal diet of mouse pellets and water for three days for acclimatization. Experimental protocol and diets: Curd probiotic containing live viable strains of naturally occurring beneficial microorganisms was used. The mice were randomly divided into four equal groups. Group A was considered as control, fed on standard mouse pellet (5g/mouse/day) and drinking water and others were supplemented with 5g curd (group B), 10g curd (group C), 20g curd (group D) per 25g of standard mouse pellet, respectively, for 45 days. Collection of blood for haematology: After completion of experimental period, blood samples were collected by sacrificing the mice. The mice were kept fasting overnight, then placed in an airtight container one by one containing cotton soaked in chloroform. They were checked for unconsciousness. The abdominal and thoracic cavities were opened and the blood was collected by a syringe directly from the heart. A portion of blood was then taken in a test tube containing 3.8% sodium citrate solution. The remaining blood was used for the collection of serum. Total erythrocyte count (TEC) and haemoglobin content (Hb) were determined as described by Shastry (1983). Preparation of serum for biochemical analysis: About 2 ml of blood was collected in sterile glass test tubes, which were placed in slanting position at room temperature for six hours. The tubes were kept overnight at 4°C. Serum was separated and centrifuged to remove unwanted blood cells where necessary. The samples were stored at -20°C. Serum lipid profile was assayed by conventional enzymatic methods on a Hitachi 911 automated analyzer from Roche Diagnostics (Laval, QC, Canada) according to the manufacturer’s specifications. Statistical analysis: The data were analysed statistically between normal and treated values by one way ANOVA with post-hoc Duncan’s multiple range test.

Source of Information: Bangl. vet. 2013. Vol. 30, No. 1, 20 – 24

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

It is concluded that the feeding of commercial ration with different level of probiotic improved TEC and Hb concentrations and decreased serum cholesterol, TG, HDL and LDL. Uric acid values were higher in treated groups B, C and D than in the control group A. Our current findings suggested and strengthened the previous findings that probiotics supplementation helps in improving normal hematology and lipid profile. Molecular mechanism of hypolipidemic effect of probiotics needs to be evaluated by advanced studies

Knowledge Management: Journal