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Determination of Morphological Variations Among Different Isolates of Trichoderma Spp. for Production of Effective Biopesticide

M. S. I. Sagar
Department of Biotechnology, Bangladesh Agricultural University Mymensingh-2202

A. K. Ghose
Department of Biotechnology, Bangladesh Agricultural University Mymensingh-2202

M. M. Rahman
Department of Biotechnology, Bangladesh Agricultural University Mymensingh-2202

M. A. Ullah
Department of Biotechnology, Bangladesh Agricultural University Mymensingh-2202

K. Miah
Department of Biotechnology, Bangladesh Agricultural University Mymensingh-2202

M. B. Meah
Department of Biotechnology, Bangladesh Agricultural University Mymensingh-2202

Abstract/ Executive Summary:

Thirty five isolates of Trichoderma species collected from seven different locations of Bangladesh were studied for morphological characters and molecular variation. Mycelial growth was measured on Potato Dextrose Agar media. The mycelial growth varied from 8.183 cm to 8.900 cm in diameter. Maximum mycelial growth was obtained by 18 isolates. In contrast the lowest mycelial growth was observed in the isolate T7. Collected isolates were categorised into groups depending on their cultural, morphological and molecular characterizations like colony colour, mycelial growth and pattern, sporulation capacity. Based on colony colour, isolates were grouped into five such as dark green, green, light green, yellowish green and whitish green. Maximum isolates were green and light green. On the basis of growth habit and colony consistency the isolates were categorized into three groups, in which most species had fast growth and were compact in appearance. Sporulation capacity of the isolates was significantly different in different isolates. The maximum numbers of spores were produced by the isolates of dark green and yellowish green colour, whereas lowest spore density was found in the isolates of green, light green and whitish green colour. The result indicating their morphological variations specially differences in sporulation capacity can widen up the chance to select the most virulent isolate for effective utilization as biopesticide.

Keywords: Trichoderma, Morphological variation, Sporulation capacity, Biopesticide

Location: IPM Laboratory, Department of Plant Pathology and Central Laboratory, Bangladesh Agricultural University, Mymensingh.

Start Date: 00-01-2007

End Date: 00-12-2007

Program Area: Pest Management

Commodity: Fungicide

Objectives:

1. To determine morphological variations specially sporulation capacity among some isolates of Trichoderma spp. for evaluating their efficiency as biopesticides.

Methodology:

The in vitro experiment was conducted at the IPM Laboratory, Department of Plant Pathology and Central Laboratory, Bangladesh Agricultural University, Mymensingh. Thirty five Trichoderma isolates collected from diverse origin of different hosts and locations in Bangladesh were evaluated in the present experiment. The experiment was designed in a Completely Randomized Design (CRD) with three replications in the laboratory. Culture of Trichoderma isolates - The isolates of Trichoderma spp. were grown on Acidified Potato Dextrose Agar (APDA) medium at 25±1ºC for studying cultural characteristics. The APDA was prepared as per standard procedure (Ashrafuzzaman, 1976). PDA media were melted in microwave oven and poured in each Petri-plate. During melting of media, twenty drops of lactic acid (95%) were added per litre of media to inhibit the growth of bacteria. Thereafter, 20 ml of the medium was poured in each petriplate (9 cm dia) and allowed to solidify under laminar airflow cabinet. When the media solidified, a 6 mm block of each Trichoderma isolates was placed at the middle of Petri-plate with three replications and was kept at 25±1⁰C. Morphological characteristics of Trichoderma isolates - Radial mycelial growth of the isolates were studied. The fungus started to grow at from 24 hours of inoculation and from that time their growth was measured by diameter measurement. Growth of Trichoderma was so fast that within 5-6 days their growth and sporulation was completed. Morphological parameters were observed and measured starting from 48 hours of inoculation. After 24, 48 and 72 hours of inoculation, the radial mycelial growth was measured as the mean of two perpendicular diameters and mean of three replications were taken as growth of each isolate.

Source of Information: J. Environ. Sci. & Natural Resources, 3(2): 115-120, 2010; ISSN 1999-7361

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

The maximum numbers of spores were produced by the isolates of dark green and yellowish green colour, whereas lowest spore density was found in the isolates of green, light green and whitish green colour. The result indicating their morphological variations specially differences in sporulation capacity can widen up the chance to select the most virulent isolate for effective utilization as biopesticide.

Knowledge Management: Journal