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Bacteriological and Pathological Investigation of Goat Lungs in Mymensingh and Determination of Antibiotic Sensitivity

M. M. Rashid
Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202.

M. J. Ferdoush
Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202..

M. Dipti
Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202.

P. Roy
Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202

M.S. Rahman
Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202.

M. I. Hossain
Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202.

M. M. Hossain
Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202.

Abstract/ Executive Summary:

The foremost important goal of the present study was to investigate the bacteriological and pathological conditions in lungs of goats slaughtered in four different slaughter houses/places of Mymensingh Sadar, Mymensingh and in addition to it, antibiotic sensitivity test of commonly used antibiotics in Veterinary practices were performed on isolated bacteria. A total of 75 lungs of slaughtered goats were examined individually and out of which 20 affected lungs were collected for histo pathology and bacterial isolation respectively from Mymensingh Sadar, Mymensingh in Bangladesh, during the period from January to May 2013.The lung lesions was grossly recorded 40% in goats (30 out of 75 lungs examined). Grossly, the lung lesions were categorized into (a) hemorrhages 35% (b) congestion 25% (c) hemorrhage and congestion 15% (d) emphysematous lung 15% and (e) hepatization in lung10%. In histo pathology, lung lesions were categorized into (a) bronchopneumonia 30%, (b) pneumonia 25% (c) hemorrhagic pneumonia 20% (d) emphysema 15%, (e) purulent pneumonia 10%. Pasteurella sp. (15%) was isolated from the lung lesions of hemorrhagic pneumonia, E. coli. (25%) from bronchopneumonia and Staphylococcus sp. (40%) from purulent pneumonia, focal pneumonia and emphysema, and mixed infection (Staphylococcus sp. and E. coli) 20%. Finally antibiotics sensitivity test was performed on isolated bacteria to which ciprofloxacin was more powerful than others (penicillin, amoxicillin, streptomycin, nalidixic acid and kanamycin) tested and the second one was oxytetracyclin.

Keywords: Bacteria, Pathology, Goat lung, Antibiotic sensitivity

Location: Mymensingh Sadar

Start Date: 00-01-2013

End Date: 00-05-2013

Program Area: Pest Management

Commodity: Goat

Objectives:

To investigate the bacteriological and pathological conditions in lungs of goats

Methodology:

Collection of samples: The samples were collected from four slaughter houses/places of Mymensingh Sadar (Masua bazar, KR market, Bolashpur and Kewatkhali). Lungs were collected from goats immediately after slaughter. A total of 20 affected lungs out of 75 examined were collected for pathological studies and 20 swabs were collected from inner core of lungs by cotton bud aseptically and immediately placed in Falcon tube containing 10ml nutrient broth for isolation of bacteria. All the samples were transferred to the Histopathology and Bacteriology Laboratory, Department of Pathology, Bangladesh Agricultural University, Mymensingh for histopathology, isolation and identification of bacteria respectively.

Gross pathology: A total of 20 samples were examined for gross abnormalities of lungs. Gross tissue changes were observed and recorded carefully, and representative tissue samples containing lesions were fixed in 10% neutral buffered formalin for histopathological studies at least for 3-7 days

.Histopathology: The formalin-fixed 20 lung tissues containing lesion were trimmed, processed, sectioned and stained as per standard procedure (Luna, 1968).

Isolation and identification of bacteria: Swabs from each of 20 lung samples were incubated for 24 hours at 37°C. From the nutrient broth, subcultures were made in nutrient agar, blood agar, and EMB agar and incubated at 37°C for overnight. The identification of the organisms was performed by the tests as described by Alam et al. (2001), Ferdausi et al. (2008) and Tonu et al. (2011). On the basis of colony and staining characters the bacteria were grouped into four types: Pasteurella sp., E. coli, Staphylococcus sp., and mixed colonies of Staphylococcus sp. and E. coli. . Two representative samples of Pasteurella sp. and three of E. coli were tested for biochemical test using sugars.

Source of Information: Bangl.J.Vet.Med. (2013).11(2): 159-166 ISSN: 1729-7893 (Print) 2308-0922 (Online)

Article Link (Online Journal):

Funding Source:

Total Budget (Tk.) :

Achievement/Findings:

A total of 75 goat lungs were examined and 30 were found to be apparently abnormal in naked eye . Out of 30 abnormal lungs, 20 were collected for pathological and bacteriological investigation. The occurrence of affected goats with lung disorders at 4 slaughter houses/places was about 40%. Grossly the lung lesions were categorized into following types: (a) Hemorrhages 35% (b) Congestions 25% (c) hepatization (red and gray) of lungs 10% , (d) hemorrhage and congestions 15% (e) Emphysema 15% . In histopathology, the lung lesions were categorized into: (a) pneumonia 25% , (b) bronchopneumonia 30%, (c) emphysema 15% , (d) purulent pneumonia 10%, and (e) hemorrhagic pneumonia 20% . The prevalence of Pasteurella sp. was 15% , E. coli 25%, Staphylococcus sp. 40% and mixed infection (Staphylococcus sp. and E. coli) 20%. Pasteurella sp. was isolated from pneumonia and hemorrhagic pneumonia. E. coli was isolated from hemorrhagic pneumonia, pneumonia (severe) and bronchopneumonia. Staphylococcus sp. isolated from emphysema, bronchopneumonia and purulent focal pneumonia.

Knowledge Management: Journal