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In Vitro Free Radical Scavenging Activity of Methanol Extract of the Leaves of Mimusops Elengi Linn.

M. R. Saha
Department of Pharmacy, Stamford University Bangladesh, Dhaka,

S. M. R. Hasan
Department of Pharmacy, Stamford University Bangladesh, Dhaka,

R. Akter
Department of Pharmacy, Stamford University Bangladesh, Dhaka,

M. M. Hossain
aDepartment of Pharmacy, Stamford University Bangladesh, Dhaka,

M. S. Alam
Department of Agricultural Chemistry, Bangladesh Agricultural University, Mymensingh

M. A. Alam
Department of Agricultural Chemistry, Bangladesh Agricultural University, Mymensingh

M. E. H. Mazumderc
Department of Pharmacy, Jahangirnagar University, Dhaka, Bangladesh

Abstract/ Executive Summary:

In the present study, antioxidant potential of the methanol extract of the leaves of Mimusops elengi Linn. was evaluated by using 1, 1-diphenyl-2-picrylhydrazyl (DPPH) scavenging assay, reducing power and total antioxidant capacity. The extract showed significant activities in all antioxidant assays compared to the reference antioxidant ascorbic acid in a dose dependent manner. In DPPH scavenging assay the IC50 value of the extract was found to be 43.26μg/ml while the IC50 value of the reference standard ascorbic acid was 58.92 μg/ml. Total antioxidant activity was also found to increase in a dose dependent manner. Moreover, M. elengi extract showed strong reducing power. These results suggest that Mimusops elengi may act as a chemopreventative agent, providing antioxidant properties and offering effective protection from free radicals.

Keywords: Mimusops elengi, Antioxidant, Reactive oxygen

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Program Area: Resource Development and Management

Commodity:

Objectives:

A study of the antioxidant activity of the leaves of M. elengi. The evaluation of antioxidant power was performed in vitro by the DPPH, reducing power and total antioxidant capacity assays.

Methodology:

Leaves of Mimusops elengi were collected from Siddeswari campus, Stamford University, Bangladesh in June 2007, and identified by Professor Dr. Abdul Ghani (Stamford University, Dhaka, Bangladesh); a voucher specimen (SU-MAA-2007-3) for this collection has been retained in the Pharmacognosy Laboratory, Stamford University, Dhaka, Bangladesh. Extraction The shade-dried leaves were coarsely powdered and extracted with 95% methanol by a Soxhlet apparatus at 45⁰C. The solvent was completely removed by rotary evaporator and obtained greenish gummy exudates. This crude extract was used for further investigation for potential antioxidant properties. The methanol extract was applied on a TLC plate as a spot (100 μg/ml) for chromatographic separation of the extract using the mobile phase methanol:chloroform (95:5, v/v). It was allowed to develop the chromatogram for 30 minutes. After completion of the chromatogram the whole plate was sprayed with DPPH (0.15 % w/v) solution using an atomizer. The color changes (yellowish color development on pinkish background on the TLC plate) were noted as an indicator of the presence of antioxidant substances. Quantitative analysis The free radical scavenging capacity of the extracts was determined using DPPH (Braca et al., 2001). DPPH solution (0.004% w/v) was prepared in 95% methanol. Metahnol extract of Mimusops elengi was mixed with 95 % methanol to prepare the stock solution (5 mg/mL). Freshly prepared DPPH solution (0.004% w/v) was taken in test tubes and Mimusops elengi extracts was added followed by serial dilutions (1 μg to 500 μg) to every test tube so that the final volume was 3 mL and after 10 min, the absorbance was read at 515 nm using a spectrophotometer (HACH 4000 DU UV – visible spectrophotometer). Ascorbic acid was used as a reference standard and dissolve in distilled water to make the stock solution with the same concentration (5 mg/mL). Control sample was prepared containing the same volume without any extract and reference ascorbic acid. 95 % methanol was served as blank. % scavenging of the DPPH free radical was measured

Source of Information: Bangl. J. Vet. Med. (2008). 6 (2): 197–202

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Achievement/Findings:

From the above results and discussion it can be concluded that the methanol extract of Mimusops elengi possesses the potent antioxidant substances which may be responsible for its anti-inflammatory and chemoprotective mechanism as well as justify the basis of using this plant’s extract as folkloric remedies.

Knowledge Management: Journal